1.一种用于对A型流感病毒流行毒株检测和分型的基因芯片，其特征是它 包括对H1N1、H3N2、H5N1、H9N2四种亚型进行检测和分型的79条特异性寡核 苷酸探针、两条质控探针和载体，两条质控探针为质控探针I和质控探针II， 其中特异性寡核苷酸探针和质控探针I分布在载体上，
对H1N1进行检测和分型的特异性寡核苷酸探针的序列
编号 名称 序列
1 H1N1-HA-1 ATGCCAACAACTCAACCGAC
2 H1N1-HA-2 ACTTGAGAAGAATGTGACAGTGAC
3 H1N1-HA-3 ACACAGAATGCCATTGACGG
4 H1N1-HA-4 GAAATTGGAAATGGCTGCTTTGA
5 H1N1-HA-5 CTATATGAGAAAGTAAGAAGCCAGC
6 H1N1-NA-1 CTAGTGGGAGCAGCATTTCTTT
7 H1N1-NA-2 TTGTGGCGTTGATAGTGATACTG
8 H1N1-NA-3 ATTCATCTCTTTGTTCTATCAGTGGA
9 H1N1-NA-4 ATACACAAAAGACAACAGCATAAGAAT
10 H1N1-NA-5 CTAATGGATGGACAGATACCGATAG
11 H1N1-NA-6 GATTGGTCAGGATACAGTGGAAG
对H3N2进行检测和分型的特异性寡核苷酸探针的序列
12 H3N2-HA-1 CTGTTACCCTTATGATGTGCCG
13 H3N2-HA-2 GAACGCAGCAAAGCCTACAG
14 H3N2-HA-3 ATTAACAGCACAGGGAATCTAATTG
15 H3N2-HA-4 CAATAATGAGATCAGATGCACCCA
16 H3N2-HA-5 GAACAAAGAAGCAACTGAGGGA
17 H3N2-HA-6 GCTGAGGATATGGGCAATGG
18 H3N2-HA-7 ATGACAACAGCACGGCAAC
19 H3N2-HA-8 CAACAGGTAGAATATGCGACAGT
20 H3N2-HA-9 CTGTTACCCTTATGATGTGCCG
21 H3N2-HA-10 TTATGCCTCCCTTAGGTCACTAG
22 H3N2-HA-11 GGATGCGGAATGTACCAGAGA
23 H3N2-HA-12 AACTAGAGGCATATTCGGCG
24 H3N2-HA-13 GGGTCAATCAGAAATGGAACTTATG
25 H3N2-NA-1 TGACCAACACCACCATAGAGAA
26 H3N2-NA-2 AGCAGAATACAGAAATTGGTCAAAG
27 H3N2-NA-3 ATGCGATCCTGACAAGTGTTATC
28 H3N2-NA-4 GGACAGGGAACAACACTAAACAA
29 H3N2-NA-5 ATGGAGTGAAAGGCTGGGC
30 H3N2-NA-6 TCCTGGTAACTACTGTAACATTGC
31 H3N2-NA-7 GCCACAATATGCTTCCTTATGC
32 H3N2-NA-8 GTATCTGACCAACACCACCATAG
33 H3N2-NA-9 CTGTGTGAACCAACAATAATAGAAA
34 H3N2-NA-10 GCTCAAGTTGTCACGATGGAA
35 H3N2-NA-11 CCTATTGATGAATGAGTTGGGTGT
36 H3N2-NA-12 GAGGCTTGTAGATAGTATTGGTTCA
37 H3N2-NA-13 CATAGTTGACAGAGGTAATAGGTCC
对H5N1进行检测和分型的特异性寡核苷酸探针的序列
38 H5N1-HA-1 CGACAGAGCAGGTTGACACA
39 H5N1-HA-2 TCAAGAAAGGGGACTCAGCAA
40 H5N1-HA-3 AAGGCAATAGATGGAGTCACC
41 H5N1-HA-4 GCCGTTGGAAGGGAATTTAATAAC
42 H5N1-HA-5 AACAAGAAGATGGAAGACGGATT
43 H5N1-HA-6 CGGAATGGTCTTACATAGTGGAG
44 H5N1-HA-7 CCAGCCAATGACCTCTGTTAC
45 H5N1-HA-8 GCTACAATAATACCAACCAAGAAGA
46 H5N1-HA-9 CCTAATGATGCGGCAGAGC
47 H5N1-HA-10 TGCCATTCCACAACATACACC
48 H5N1-HA-11 AAGTGAATTGGAATATGGTAACTGC
49 H5N1-HA-12 CCGCAGTATTCAGAAGAAGCAA
50 H5N1-HA-13 TTATTCAACAGTGGCGAGTTCC
51 H5N1-HA-14 AAGAACTGAAACACCTATTGAGCA
52 H5N1-NA-1 TGGAGGAACAACATACTGAGAAC
53 H5N1-NA-2 GGCATAATAACAGACACTATCAAGA
54 H5N1-NA-3 AGCACTAATTCCAGGAGCGG
55 H5N1-NA-4 CCAGAACTGACAGGATTAGATTGC
56 H5N1-NA-5 ATTGGTCAGGATATAGCGGGAG
57 H5N1-NA-6 GCCGTTCATCTCATGCTCC
58 H5N1-NA-7 TTGACTCAGGGAGCCTTGC
59 H5N1-NA-8 TGGAGGAACAACATACTGAGAAC
60 H5N1-NA-9 TTACTGTAATGACTGATGGACCAAG
61 H5N1-NA-10 TTCGGAGACAATCCACGCC
62 H5N1-NA-11 TGGCATGGCTCAAATCGGC
63 H5N1-NA-12 AGCACTAATTCCAGGAGCGG
64 H5N1-NA-13 GGTGGACTGGAACGGACAG
对H9N2进行检测和分型的特异性寡核苷酸探针的序列
65 H9N2-HA-1 GCATCGGCTACCAATCAACAA
66 H9N2-HA-2 TGTTCCTGTGACACATGCCA
67 H9N2-HA-3 ATGCCAAAGAATTACTCCACACA
68 H9N2-HA-4 AGAATTGCTAGTGCTGCTTGAA
69 H9N2-HA-5 CAGGTCAGACATTGCGAGTG
70 H9N2-HA-6 ATGGTATGGACACATTCTTTCAGG
71 H9N2-HA-7 ACAAGGACTGACACAACAAC AA
72 H9N2-HA-8 AGGTCAGACATTGCGAGTAAGA
73 H9N2-HA-9 TCTATGGCAATCCTTCCTGTGA
74 H9N2-HA-10 TCGGATTCATTCTACAGGAGCA
75 H9N2-HA-11 AATACCACATTGCCATTCCACA
76 H9N2-NA-1 GGTATTCTGGTATCTTCTCTGTTGA
77 H9N2-NA-2 CCACGGCTAATTCCAAGTCAC
78 H9N2-NA-3 AAGTGGCAGAATACAAGAATTGGT
79 H9N2-NA-4 GTCAAATTACAGGGTTCGCTCC
两条质控探针的序列
80 I CGTATATAAAACGGAACGTCGAAGG
81 II CCTTCGACGTTCCGTTTTATATACG-cy3
。
