[发明专利]一种类人胶原蛋白及其生产方法

摘要

本发明公开一种类胶原蛋白及通过基因工程菌生产类胶原蛋白的方法。其类人胶原蛋白的生产方法包括类人胶原蛋白的工程菌的构建;将工程菌的发酵培养;再经类人胶原蛋白的诱导及表达,提纯,获得目的蛋白。该方法获得的重组蛋白在单细胞内的表达高,其类胶原蛋白为螺旋结构,异于动物体原蛋白的交叉结构,因此它具有很好的可加工性而不改变其分子量,因此类人胶原蛋白可加工成手术缝合线、人工皮肤、胶片余层,人工器官涂层等,也可与卤化银、染料等结合形成具有优良表面粘性的涂料。

主权项

1、一种类人胶原蛋白，其特征在于：其序列如下HDPVVLQRRDWENPGVTQLNRHLAHAHPPFASDHPMGAPGPAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAHGPAGALGAHGPAGPLGPAGPPGSAGAPGAHGPAGPLGAHGPAGPLGAHGPAGPLGAHGPAGPLGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAHGPAGPLGAHGPAGPLGAHGPAGPLGAHGPAGPLGAPGPAGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAHGPAGPLGAHGPAGPLGAHGPAGPLGAHGPAGPLGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAHGPAGPLGAHGPAGPLGAHGPAGPLGAMGAPGATGLSAGATHGLVTCGL所述结构为三链、三螺旋结构，其核酸长度为1071bp。