[发明专利]一种锌指蛋白质及其抗体的制备和应用无效
| 申请号： | 02115935.1 | 申请日： | 2002-06-07 |
| 公开（公告）号： | CN1422864A | 公开（公告）日： | 2003-06-11 |
| 发明（设计）人： | 李文鑫;孙燕;苟德明;高立 | 申请（专利权）人： | 武汉大学 |
| 主分类号： | C07K14/435 | 分类号： | C07K14/435;C07K16/18;C12N15/12;A61K38/17;A61K39/395;G01N33/68;A61P7/00 |
| 代理公司： | 武汉科宏专利事务所 | 代理人： | 黄瑞棠 |
| 地址： | 430072*** | 国省代码： | 湖北;42 |
| 权利要求书： | 查看更多 | 说明书： | 查看更多 |
| 摘要： | 本发明涉及一种锌指蛋白质及其抗体,具体地说,涉及一种与人的造血系统发育有关的蛋白质及其抗体。本发明提供的ZNF268蛋白质,是一个典型的krüppel型C2H2锌指蛋白质；其制备主要是构建ZNF268蛋白质的表达载体,转入宿主细胞,在适合的条件下,表达及分离纯化ZNF268蛋白质；可用于诊断或治疗因该蛋白质异常而引起的疾病。ZNF268蛋白质的抗体,可以特异性地与ZNF268蛋白质结合；其制备主要是将ZNF268蛋白质与佐剂混合免疫动物,再分离免疫动物血液,纯化抗体；可用于诊断、治疗或预防与ZNF268蛋白质的异常表达相关的疾病。 | ||
| 搜索关键词： | 一种 蛋白质 及其 抗体 制备 应用 | ||
【主权项】：
1、一种ZNF268蛋白质，其特征在于，是一个典型的krüppel型C2H2锌指蛋白质，序列为：MetAlaThrArgValArgThrAlaSerIle10TrpValProProLeuGlnGluArgAsnSer20SerTrpAspArgIleArgLysLeuGlnGly30GlnGluSerIleLeuGlyGlnGlyThrPro40GlyLeuGlnProLeuProGlyThrProArg50GlnLysGlnLysSerArgArgIleGluLys60ValLeuGluTrpLeuPheIleSerGlnGlu70GlnProLysIleThrLysSerTrpGlyPro80LeuSerPheMetAspValPheValAspPhe90ThrTrpGluGluTrpGlnLeuLeuAspPro100AlaGlnLysCysLeuTyrArgSerValMet110LeuGluAsnTyrSerAsnLeuValSerLeu120GlyTyrGlnHisThrLysProAspIleIle130PheLysLeuGluGlnGlyGluGluLeuCys140MetValGlnAlaGlnValProAsnGlnThr150SerGlyAspThrValTrpLysIleAspAsp160LeuMetAspTrpHisGlnGluAsnLysAsp170LysLeuGlySerThrAlaLysSerPheGlu180CysThrThrPheGlyLysLeuCysLeuLeu190SerThrLysTyrLeuSerArgGlnLysPro200HisLysCysGlyThrHisGlyLysSerLeu210LysTyrIleAspPheThrSerAspTyrAla220ArgAsnAsnProAsnGlyPheGlnValHis230GlyLysSerPhePheHisSerLysHisGlu240GlnThrValIleGlyIleLysTyrCysGlu250SerIleGluSerGlyLysThrValAsnLys260LysSerGlnLeuMetCysGlnGlnMetTyr270MetGlyGluLysProPheGlyCysSerCys280CysGluLysAlaPheSerSerLysSerTyr290LeuLeuValHisGlnGlnThrHisAlaGlu300GluLysProTyrGlyCysAsnGluCysGly310LysAspPheSerSerLysSerTyrLeuIle320ValHisGlnArgIleHisThrGlyGluLys330LeuHisGluCysSerGluCysArgLysThr340PheSerPheHisSerGlnLeuValIleHis350GlnArgIleHisThrGlyGluAsnProTyr360GluCysCysGluCysGlyLysValPheSer370ArgLysAspGlnLeuValSerHisGlnLys380ThrHisSerGlyGlnLysProTyrValCys390AsnGluCysGlyLysAlaPheGlyLeuLys400SerGlnLeuIleIleHisGluArgIleHis410ThrGlyGluLysProTyrGluCysAsnGlu420CysGlnLysAlaPheAsnThrLysSerAsn430LeuMetValHisGlnArgThrHisThrGly440GluLysProTyrValCysSerAspCysGly450LysAlaPheThrPheLysSerGlnLeuIle460ValHisGlnGlyIleHisThrGlyValLys470ProTyrGlyCysIleGlnCysGlyLysGly480PheSerLeuLysSerGlnLeuIleValHis490GlnArgSerHisThrGlyMetLysProTyr500ValCysAsnGluCysGlyLysAlaPheArg510SerLysSerTyrLeuIleIleHisThrArg520ThrHisThrGlyGluLysLeuHisGluCys530AsnAsnCysGlyLysAlaPheSerPheLys540SerGlnLeuIleIleHisGlnArgIleHis550ThrGlyGluAsnProTyrGluCysHisGlu560CysGlyLysAlaPheSerArgLysTyrGln570LeuIleSerHisGlnArgThrHisAlaGly580GluLysProTyrGluCysThrAspCysGly590LysAlaPheGlyLeuLysSerGlnLeuIle600IleHisGlnArgThrHisThrGlyGluLys610ProPheGluCysSerGluCysGlnLysAla620PheAsnThrLysSerAsnLeuIleValHis630GlnArgThrHisThrGlyGluLysProTyr640SerCysAsnGluCysGlyLysAlaPheThr650PheLysSerGlnLeuIleValHisLysGly660ValHisThrGlyValLysProTyrGlyCys670SerGlnCysAlaLysThrPheSerLeuLys680SerGlnLeuIleValHisGlnArgSerHis690ThrGlyValLysProTyrGlyCysSerGlu700CysGlyLysAlaPheArgSerLysSerTyr710LeuIleIleHisMetArgThrHisThrGly720GluLysProHisGluCysArgGluCysGly730GluLysProHisGluCysArgGluCysGly730LysSerPheSerPheAsnSerGlnLeuIle740ValHisGlnArgIleHisThrGlyGluAsn750ProTyrGluCysSerGluCysGlyLysAla760PheAsnArgLysAspGlnLeuIleSerHis770GlnArgThrHisAlaGlyGluLysProTyr780GlyCysSerGluCysGlyLysAlaPheSer790SerLysSerTyrLeuIleIleHisMetArg800ThrHisSerGlyGluLysProTyrGluCys810AsnGluCysGlyLysAlaPheIleTrpLys820SerLeuLeuIleValHisGluArgThrHis830AlaGlyValAsnProTyrLysCysSerGln840CysGluLysSerPheSerGlyLysLeuArg850LeuLeuValHisGlnArgMetHisThrArg860GluLysProTyrGluCysSerGluCysGly870LysAlaPheIleArgAsnSerGlnLeuIle880ValHisGlnArgThrHisSerGlyGluLys890ProTyrGlyCysAsnGluCysGlyLysThr900PheSerGlnLysSerIleLeuSerAlaHis910GlnArgThrHisThrGlyGluLysProCys920LysCysThrGluCysGlyLysAlaPheCys930TrpLysSerGlnLeuIleMetHisGlnArg940ThrHisValAspAspLysHis***948
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