[发明专利]鉴别显著影响猪背膘厚性状的硬脂酰辅酶A去饱和酶基因单核苷酸多态位点及应用

摘要

本发明公开了一个鉴别显著影响猪背膘厚性状的硬脂酰辅酶A去饱和酶基因单核苷酸多态位点及应用，它先进行PAC文库的筛选，再经过猪全长SCD基因的克隆、猪全长SCD基因mRNA的分离、外显子2上SNP C3101T的鉴别，最后经过PCR－RFLP法等方法进行SNP C3101T基因型判定。本发明通过现代分子生物学技术检测该多态位点，利用标记辅助选择(MAS)选择有利的等位基因型个体留种，可以显著改善种猪群体背膘厚性状，提高瘦肉产出率。

主权项

1、一个鉴别显著影响猪背膘厚性状的硬脂酰辅酶A去饱和酶基因单核苷酸多态位点，其特征在于：该多态位点序列如下： 1 tctagaccct ttgaatttcc acttaaattt tagaatctgc ttgtctattt cctcaaagaa 61 atctgttgga tttctatagt gattaaatta atctatagat caacactgag tctgtccatt 121 tatgaacaca gaatataaca aattcttttt ttctctctat ttaagagttt taaagttttt 181 ctcagcattg ttttgatact ttcagtgtag ctatcctata aacatttaga tttattcttt 241 gtgacatttt tgatgttctt ttaatttttg tttttttagg gccgcacctg tggcacatga 301 agttcctggg ccaggggtcg aatcagagcc aaagctacca gcctatacca ccaccatagc 361 aacgccaggt ctggaccgca tctgtgatgt acgccggagc tcgtgacaat gccagatcct 421 taacccactg agcaaggcca gcatgtaacc cacaacctcg tggacactag tcgagttctt 481 aacccgctga gccacaagga gaactccatt tttgatgcta ttttaaacag tatattttaa 541 atttcattct gcaattgttt tttaatagaa tataggacag aataattttt ttatactggt 601 ttctatcttg acctttctaa attcacttac caattttgac acttttttgg tggtagattc 661 tatacatttt tcatacacac agttaggtct tctgcaaata ataacagttt ctttcttctt 721 ttcctatctt tatatctttc atttcttttt tcctgtccat catgcaacct aggatttcta 781 gcacaatgtt gactagaaat ggtagacgat tatatgcttg cctgttctct taaggggaaa 841 tttttttttt ttttttttgg tctttttaag cctgcaccca tggctaggaa ctgtagctgc 901 tggcctatgc cacagccaca gcaacactgg atccgaactg tatctgtgac ctacaccaca 961 gctcatggct atgccagatc cttaacccac tgagcgaggc cagggatcga accctcgacc1021 tcacggttcc tagtcggatt cgtctccgct gcgccacaac gggaactccc acgactaatg1081 attaagtaaa atgattcttg tggactgact tgcctaataa ttctatagct gatagctatt1141 tttgtcccac ttacaagtcc ttttgcctgc ttctggatga aggcacacgc tgcgcttcct1201 cccttttctc atgaaagcca ttccaaaatt acaactcgac aatctgaaga atttccaaaa1261 catcccgcac acgttcgggg tggggggggg gcgtgggcac aaggacggga tcgctcactg1321 gtgcctggcg gcgaacgtcc cctgctgggc ttgcctagta gctgaacgcc gtgcgcgcgt1381 gcaggtccac acatctatct atgcatctac gtgtgtgtac acggctgccg gcctggagac1441 cggcgagttc agcagtacgc atctatcgcc ccgccgccag tgggcggcgc agagccattg1501 ttccgccgcc ggccgaccct cagcccctgc ggcacgcgcg ttcgtcacgg agggaggtgg1561 ggcttcgccg ggcccccggc ccccaacccg gggtttgaag gcacgtctcc ccctcccacg1621 cgtctccgcg agctagaagg cgggagtgga gttgtgcgta gcggtcgccc gcgcggcttc1681 acccacactt tccccggaaa cttccctagt gcccatcctt tcgcgaagtg ccccagggcc1741 agtcctgggc tagcacccac ctcccacccc cctactgagt cctcttcctc ttccagggaa1801 gacgctctcg agcgcctccg aggaggcagg acgccgggca gaagcccagc ggccggtgga1861 agagaagctg aggaggagaa agggagggga gggggagtga ggagctcgcg gcagagggaa1921 cagcagattg cgccgagcca atggcaacgg caggacgagg tggcaccaaa ttcccttcgg1981 ccaatgacgc gccagagtct acagaagccc attagcattt ccccaggggc aggggcagag2041 gcaggggctg cggagaccga gccgcggagt gtctgcagca tccagttttt gcgtctccgg2101 cccccagcaa gccccggctc tctgtctcct cccctctccc gcccatgcgg ttctcccacg2161 gtgagccaac tctgcgcact ttgccccttc ttggcagcga ataaaagggg tcagaggaaa2221 tacaggatac ggtcgcccac tgccagctct agcctttaaa tacccagcct ggggagaccc2281 acgcacagca ggtcgggtgc agacaccgac ccaacgtgca gcaaagggtt ccgagcgcag2341 catcgctgat ctccgcgcaa gagccggctc cagcactagt ctacgctcag tgcggtctgc2401 cacgaactcc tccctgaagt ctcatccctg ggaaagtgat cccaacgtcc gagagcccag2461 atgccggccc acttgctgca agaggaggtg agtttccaag taatggcccc cagaccgcgg2521 gctcgccagt gctggttggg cttctaggtg actcggtgta gaagagagtt gagatcgccg2581 aggacagcca cctttttcga gttgtatagt cttcagtttg ttgggaatgt ggattgtaat2641 ttgggaactt agttctccaa cttttgtttc ctaaagcttc aaagaaaaat ctggtggtac2701 ctgtaccctg atgtgtgtag gcatgcgggc tttcctttgt gtgtttgaag gggtgcgagt2761 gttttacccc tctatttccc cttcccctga ggtttcaccc tcctccccaa tttctccgca2821 cagttcctgg ggacttgcgt ctcttccatc taggatagac cttgccctca ggtaacctgg2881 gtattggggc caggtctgtg cctatacccc gcccttcccc cagctagacc agggagtgaa2941 gtggccccta gtgtctccac gtgtcctccc ctctctccca gctctgcact tccactctct3001 ttcagatctc tagctcctac acaaccacca ctaccatcac agcaccttcc tccagggtcc3061 tgcagaatgg agggggcaag ttggagaaga ctccccaata cgtggaagaa gacatccgcc3121 ctgaaatgaa agatgacatc tatgacccaa cctaccagga taaggagggc ccaaggccca3181 agcttgaata tgtttggaga aacatcatcc tcatgagtct gctacacttg ggagccctgt3241 atgggatcat attgatcccc acctgcaaga tatacacctt gctctggggt aagaggtccc3301 cttgtcctcc tgtctcagta tcccaggttc tctccactct tttaatacag tagcaactta3361 cacagagtag aaacaccagc ccctccaggc cgaggttttt ccaaccattt ctcttaggtg3421 ttgtggtggg ctgggaaggg agtctagggg gtttgggatg caggagtgtc gattctgggg3481 cctttaattt gggggggggg ttggagaggg agccctggga ccatgggcca caggctttga3541 agtgagtaga tctgtgccag ggagtcagct tttctgtttc aagtcatttt cttgactgct3601 tgtcccagcc ctgccagcct agaaagaatc aaggccctta cctggatgca tgggaacatg3661 gggctagact agtgtgactt tagagggcac atgctgggta tagtcacggg gtagcaagag3721 aaactactca ggaagagggt taagcagaag aggggggaag agagctgtgg tcatccatga3781 aattattttg ccctgatcct tagccttatc aggctcctta attacacaga cagaggaagg3841 cggctcctgc ataactcatg ttacatggat gtcttgctct gtgactccac gtgggtctcc3901 agggaactgc acatgccact caagaaaatg ttctaatgaa ataaggttaa agaaacgttt3961 cctgccccat tccccaccca tcctgctcca gcccctctcc cctgatgcca ggagtgcctc。