[发明专利]中国葡萄属野生种毛葡萄抗黑痘病候选基因序列及应用

摘要

本发明涉及毛葡萄常规杂交育种、分子标记辅助育种、基因定位与作图及基因克隆的方法与技术。采用抗病基因同源序列法(Resistant geneanalogus，RGAs)获得中国葡萄属野生种毛葡萄(V.quinquangularis Read)南郑－1、泰山－12、83－4－96等3个株系抗黑痘病候选基因DNA片段26个，对这些片段克隆测序后，其大小介于492bp～550bp之间。以这些序列为依据，可以进行毛葡萄抗黑痘病标记辅助育种，用作葡萄育种中检测毛葡萄抗黑痘病基因的探针；可以根据这些片段在毛葡萄与欧洲葡萄杂种后代中的分离情况，进行抗黑痘病基因定位与作图；也可以根据这些片段设计特异引物，采用cDNA末端快速扩增技术(Rapid amplification of cDNA ends，RACE)(Frohman et al，Proc.Natl.Acad.Sci，1988)克隆毛葡萄抗黑痘病基因，为通过基因工程改良葡萄品种的抗病性奠定基础。

主权项

1.中国葡萄属野生种毛葡萄抗黑痘病候选基因序列，其特征在于，包括南郑-1株系、泰山-12株系、83-4-96株系共26个中国葡萄属野生种毛葡萄的抗黑痘病候选基因序列，这些抗黑痘病候选基因序列分别是：1)南郑-1株系的11个抗黑痘病候选基因序列：毛葡萄南郑-1(2)1 GGTGTTGGGA AGACAACTTT CGCCAAAGAA GTTTACGACA ACCAGAGAGT GGTGGGACAC61 TTCGATTGCC ATGCTTGGGT CACTGTGTCT CAATCATTCA AGATGGAAGA GCTACTACGG121 AACATGACAA AGAAATTCTA TCAGGGAAGA AAGGAAGCAG TTCCTGAGGG TATCGATACA181 ATAGACGAGA TGTCACTGAT TGCCTTAATC AGGCAGTATT TACAAGATAA AAGGTATGTG241 GTTGTTTTTG ATGATGTATG GAAACTAGAT TTCTGGGGAT TTATTAAATA TGTTTTACCA301 GAAAATGGGA AGGGCAGTAG GATAATAATC ACTACACGTA ATGATGAGGT AGCCTCTTCT361 TGCAAAGAGT CGTCATTTGA TTACATCCAC AAGCTGCAAC CCCTGTCCCC AAAGAGTTCC421 TGGGAACTCT TTTGCAAGAA GACATTCCAG AGAGGATGTC CTCCAGAGTT GGAGAAACTG481 TCTCTTGATA TTGTCAAAAG ATGTGGAGGA TTGCCACTAG CGTTG毛葡萄南郑-1(6)1 GGGGTTGGGA AGACAACGCT CGCACGGCTT GTTTACAATG ATGATTTGGC CAAAAATTTT61 GAACTAAAAG CGTGGGTTTG TGTGACTGAT GTGTTTGATG TGGAGAATAT AACAAAAGCA121 ATTCTCAATT CGGTGTTAAA TTCTGATGCA AGCGGCTCTT TGGACTTCCA ACAAGTTCAA181 CGGAAATTGA CAGTCACATT AGCAGGGAAA ACTCTTTTAC TCATTCTAGA TGACGTGCGG241 AACGAAAATT ATTGTAATTG GGATCGTTTG CGGGCTCCTT TGAGTGTAGT GGCAAAAGGA301 AGCAAAGTCA TAGTTACAAC ACGCAATAAA AATGTTGCAT TGATGATGGG AGCTGCCAAG361 AATGTTCATG AACTAAACCC TTTATCTGAA GATGATTGTT GGTCTGTGTT TGAGAAGCAT421 GCATTTGAAC ATAGAAATAT CAATGATCGT CCGAATCTGG TGTCCATTGG TAGGAAAATT481 GTTGCCAAAT GTGGTGGATT GCCACTAGCG TTG毛葡萄南郑-1(1)1 GGTGTTGGCA AGACAACCTT AGCAAAGTTA GTCTACAATA ATAATGCTGT TGTTGATCAT61 TTCCCTTATT GTGCTTTTGT GTCTTGTAAC TATAAGGGAA GCTCTCGAGG TCATAGCTAA121 ACAAATGGGC AATCCAAGTG CATGGAAAGA AGACAACAGA AGCAATGTAG AAAAAGCAGA181 AGTGGAAGGG TAATGGTCTG AAGAAGGCTC TTGGTCACAA CGAGTGAAGT CGATGCCTTC241 TTTAAAAACT TTCTTGAAGA ATAAGAAGCA TCTTATAGTT TTGGATGACA TAGAAATGGA301 TGCTGGCTTG AATATCTTGC TTAGCAAACT CCCAAAAACA TCAAATGGGA GTCGGATGAT361 CTTGACCACT CGGTATGCGA GGGGACTTGC AGATCTACAG AAGAAGGGCA CTAATCTAAC421 ACTGCAATTA CATGATGATG ATGAGAGTTG GATGCTGTTC AAACATATTT TGAAGGTAGA481 CATACCCCAA GAACTGATAT ATCTTAGAAG AGACATTGTG ACAACATGCT GTGGATTGCC541 ACTAGCGTTG毛葡萄南郑-1(12)1 GGGGTTGGGA AGACTACCCT CATGACCCAA GTCAACAACG AGTTCCTTAA AACTATTCAT61 CAATTTGACA TAGTGATTTG GGTTGTGGTT TCCAGAGACC CAAATCCCGA AAAGGTTCAG121 GATGAAATTT GGAAGAAGGT AGGGTTTTGC GATGATATAT GGAAAAGCAA AAGCCAAGAT181 GAGAAAGCCA TTAGCATATT CAGGATCCTG GGCAAGAAAA AGTTTGTTTT GTTCTTGGAT241 GATGTGTGGG AGCGGTTTGA TCTCTTAAAG GTGGGGATTC CACTGCCAAA CCAACAAAAT301 AATTCCAAGG TTGTATTTAC GACACGATCT GAAGAGGTTT GTGGCCGCAT GGGAGCTCAC361 AGGCGAATAA AAGTTGAGTG TTTGGCATGG AAGCAAGCCT GGGACTTGTT TCAAAACATG421 GTAGGAGAAG ATACCCTTAA TTCTCATCCT GAAATACCTC AGCTAGCTGA AACTATTGTG481 AAGGAGTGTC TCGGATTGCC ACTAGCGTTG毛葡萄南郑-1(14)1 GGGGTTGGTA AGACTACGCT CGCACGGCTT GTTTACAATG ATGATTTGGC CAAAAATTTT61 GAACTAAAAG CGTGGGTTTG TGTGACTGAG GTTTTTTATG TGGAGAATAT AACAAAAGCA121 ATTCTCAATT CGGTGTTAAA TTCTGATGCA AGTGGCTCTT TGGACTTCCA ACAAGTTCAA181 AAGAAATTGA CAGACACTTT AGCAGGGAAA ACTCTTTTTC TCATTCTAGA CGACGTGTGG241 AACGAAAATT ATTGTAATTG GGATCGTTTG CGGGCTCCTT TGAGTGTAGT GGCAAAAGGA301 AGCAAAGTCA TAGTTACAAC ACGCAATAAA AATGTTGCAT TGATGATGGG AGCTGCCGAG361 AATGTTCATG AACTAAACCC CTTATCAGAA GATGCTTGTC GGTCTGTGTT TGAGAAGCAT421 GCATTTGAAC ATAGGAATAT GAAAGATCAT CCAAACTTGG TGTCCATTGG TAGGAAAATT481 GTTGGCAAGT GTGGGGGATT GCCACTAGCG TTG毛葡萄南郑-1(16)1 GGAGTTGGCA AGACTACTTT CGCCAAAGAA GTTTACGACA ACCAGAGAGT GGTGGGACAC61 TTCGATTGCC ATGCTTGGGT CACTGTGTCT CAATCATTCA AGATGGAAGA GCTACTACGG121 AACATGACAA AGAAATTCTA TCAGGGAAGA AAGGAAGCAG TTCCTGAGGG TATCGATACA181 ATAGACGAGA TGTCACTGAT TGCCTTAATC AGGCAGTATT TACAAGATAA AAGGTATGTG241 GTTGTTTTTG ATGATGTATG GAAACTAGAT TTCTGGGGAT TTATTAAATA TGTTTTACCA301 GAAAATGGGA AGGGCAGTAG GATAATAATC ACTACACGTA ATGATGAGGT AGCCTCTTCT361 TGCAAAGAGT CGTCATTTGA TTACATCCAC AAGCTGCAAC CCCTGTCCCC AAAGAGTTCC421 TGGGAACTCT TTTGCAAGAA GACATTCCAG GGAGGATGTC CTCCAGAGTT GGAGAAACTG481 TCTCTTGATA TTGTCAAAAG ATGTGGAGGA TTGCCACTAG CGTTG毛葡萄南郑-1(18)1 GGTGTGGGGA AAACAACGCT CGCACGGCTT GTTTACAATG ATGATTTGGC CAAAAATTTT61 GAACTAAAAG CGTGGGTTTG TGTGACTGAT GTGTTTGATG TGGAGAATAT AACAAAAGCA121 ATTCTCAATT CGGTGTTAAA TTCTGATGCA AGTGGCTCTT TGGACTTCCA ACAAATTCAA181 AGGAAATTGA CAGACACGTT AGCAGGGAAA ACTCTTTTTC TCATTCTAGA CGACGTGTGG241 AACGAAAATT ATTGTAATTG GGATCGTTTG CGGGCTCCTT TGAGTGTAGT GGCAAAAGGA301 AGCAAAGTCA TAGTTACAAC ACACAAAAAA AATGTTGCAT TGATGATGGG AGCTGCCAAG361 AATGTTCATG AACTAAACCC TTTATCTGAA GATGATTGTT GGTCTGTGTT TGAGAAGCAT421 GCATTTGAAC ATAGAAATAT CAATGATCAT CCGAATCTGG TGTCCATTGG TAGGAAAATT481 GTTGGCAAAT GTGGTGGATT GCCACTAGCG TTG毛葡萄南郑-1(3)1 GGGGTAGGTA AGACAACTCT CGCACGGCAG GTTTACAAGT ATGATTTGGC CAAAAATCTT61 GAACTAAAAG CGTGGGTTTG TGTGACTGAT GTGTTTGATG TGGAGAATAT AACAAAAGCA121 ATTCTCAATT CGGTGTTAGA GTCTGATGCA AGTGGCTCTT TGGACTTCCA ACAAGTTCAA181 AAGAAATTGA CAGACACGTT AGCAGGGAAA ACTTTTTTAC TCGTTCTAGA CGACGTGTGG241 AACGAAAATT GTGGTCATTG GGATCTTTTG CGGGCTCCTT TGAGTGTAGG GTCCAAAGGA301 AGCAAAGTCA TAGTTACAAC ACGCAATAAA AATGTCGCAT TGATGATGGG AGCTGCTAAG361 AATGTTCATG AACTAAACCC TTTATCTGAA GATGCTTGTT GGTCTGTGTT TGAGAAGCAT421 GCATTTGAAC ATAGAGATAT CAATGATCAT CCGAACCTGG TGTCCATTGG TAGGAAAATT481 GTTGGCAAAT GTGGTGGATT GCCACTAGCG TTG毛葡萄南郑-1(4)1 GGGGTGGGGA AGACAACGCT CGCACGGCTT GTTTACAATG ATGATTTGGC CAAAAATTTT61 GAACTAAAAG CGTGGGTTTG TGTGCCTGAT GTGTTTGATG TGGAGAATAT AACAAAAGCA121 ATTCTCAATT CGGTGTTAGA TTCTGATGCA GGCGGCTCTT TGGACTTCCA ACAAGTTCAA181 CGGAAATTGA CAGTCACATT AGCAGGGAAA ACTCTTTTAC TCATTCTAGA TGACGTGCGG241 AACGAAAATT ATTGTAATTG GGATCGTTTG CGGGCTCCTT TGAGTGTAGT GGCAAAAGGA301 AGCAAAGTCA TAGTTACAAC ACGCAAAAAA AATGTTGCAT TGATGATGGG AGCTGCCAAG361 AATGTTCATG AACTAAACCC TTTATCTGAA GATGATTGTT GGTCTGTGTT TGAGAAGCAT421 GCATTTGAAC ATAGAAATAT CAATGATCGT CCGAATCTGG TGTCCATTGG TAGGAAAATT481 GTTGGCAAAT GTGGTGGATT GCCACTAGCG TTG毛葡萄南郑-1(5)1 GGGGTGGGTA AGACTACTCT CGCACGGCAG GTTTACAAGT ATGATTTGGC AAAAAATTTT61 GAACTAAAAG CGTGGGTTTG TGTGACTGAT GTGTTTGATG TGGAGAATAT AACAAAAGCA121 ATTCTCAATT CGGTGTTAGA GTCTGATGCA AGTGGCTCTT TGGACTTCCA ACAAGTTCAA181 AAGAAATTGA CAGACACGTT AGCAGGGAAA ACTTTTTTAC TCGTTCTAGA CGACGTGTGG241 AACGAAAATT GTGGTCATTG GGATCTTTTG CGGGCTCCTT TTAGTGTAGG GTCCAAAGGA301 AGCAAAGTCA TAGTTACAAC ACGCAATAAA AATGTCGCAT TGATGATGGG AGCTGCCAAG361 AATGTTCATG AGCTAAACCC TTTATCTGAA GATGCTTGTT GGTCTGTGTT TCAGAAGCAT421 GCATTTGAAC ATAGAAATAT CAATGATCAT CCGAACCTGG TGTCCATTGG TAGGAAAATT481 GTTGGCAAAT GTGGTGGATT GCCACTAGCG TTG毛葡萄南郑-1(8)1 GGGGTTGGGA AGACAACTTT CGCCAAAGAA GTTTACGACA ACCAGAGAGT GGTGGGACAC61 TTCGATTGCC ATGCTTGGGT CACTGTGTCT CAATCATTCA AGATGGAAGA GCTACTACGG121 AACATGACAA AGAAATTCTA TCAGGGAAGA AAGGAAGCAG TTCCTGAGGG TATCGATACA181 ATAGACGAGA TGTCACTGAT TGCCTTAATC AGGCAGTATT TACAAGATAA AAGGTATGTG241 GTTGTTTTTG ATGATGTATG GAAACTAGAT TTCTGGGGAT TTATTAAATA TGTTTTACCA301 GAAAATGGGA AGGGCAGTAG GATAATAATC ACTACACGTA ATGATGAGGT AGCCTCTTCT361 TGCAAAGAGT CGTCATTTGA TTACATCCAC AAGCTGCAAC CCCTGTCCCC AAAGAGTTCC421 TGGGAACTCT TTTGCAAGAA GACATTCCAG GGAGGATGTC CTCCAGAGTT GGAGAAACTG481 TCTCTTGATA TTGTCAAAAG ATGTGGAGGA TTGCCACTAG CGTTG2)泰山-12株系的抗黑痘病候选基因序列：毛葡萄泰山-12(5)1 GGGGTTGGTA AGACAACACT CGCACGGCTG CTTTACAACA ATGACATGGC CAAAAACTTT61 AACCTAAAAG CATAGGTTTG TGTGTCTGAT GTGTTTGATG TGGAAAATAT AACAAAAGCA121 ATTTTCAATT CGGTGGTAAG TTCTGATGCA GGTGGCTCGT TCCAATAGGT TCAAAAGAAA181 TTGACAGAGG CATTGATAGG GAAAAAGTTT TTACTCATTC TAAACGATGT ATGGAACCAA241 GATTATGGTA GTTGGGATAG CTTGCGCCCT CCTTTTATTG GAGGTACAAA AGGAAGCAAA301 GTCATAGTCA CAACACGCAA TAAAAACGTT GCATTGATGA TGGGAGCTGA GGAGAAGGTT361 TATGAACTAA AAACTTTATC AGAAGATGCT TGTTGGTCGA TGTTTAAGAA GCATGTGTTT421 GAACATAGAA ATATTGACGA TCATCCAAAC CTAGTGTCTA TTGGTAGGAA ATTTGTTAGC481 AAGTGTGGTG GATTGCCACT AGCGTTG3)83-4-96株系的14个抗黑痘病候选基因序列：毛葡萄83-4-96(2)1 GGGGTGGGGA AGACAACCCT TTTGACCCAA ATCAACAATG AGTTCCTCAA AACAACTCAT61 GATTTTGATG TTGTGATCTG GGCTGTGGTG TCACGAGATC CAGACTTTCC CAAGGTTCAA121 GATGAAATTG GGAAAAAGGT TGGGTTTTGT GATAGTATAT GGAGAAATAA AAGCAAGGAT181 GAAAAAGCCA TAGACATCTT TAGAGCCTTG CGCAAAAAGA GGTTTGTGTT GTTGTTAGAT241 GACGTATGGG AGCCGGTAAA TCTATCAGTC TTGGGAGTTC CAGTTCCAAA TGAAGAAAAT301 AAGTCCAAGC TAGTATTCAC TACCCGATCC GAGGATGTAT GCCGACAAAT GGAAGCTCAA361 AAGAATATCA AAGTGGAGTG CTTGGCATGG CAGGAATCCT GGGATTTGTT TCAGAAGAAG421 GTTGGACAAG ACACACTTGA CTCCCATGCA GAAATCCCCA TGCTAGCTGA AATGGTTGCC481 AAAGAATGTT GCGGATTGCC ACTAGCGTTG毛葡萄83-4-96(3)1 GGTGTTGGTA AGACTACCCT CATGACCCAA GTCAACAACG AGTTCCTTAA AACTATTCAT61 CAATTTGACA TAGTGATTTG GGTTGTGGTT TCCAGAGACC CAAATCCCGA AAAGGTTCAG121 GATGAAATTT GGAAGAAGGT AGGGTTTTGC GATGATAAAT GGAAAAGCAA AAGCCAAGAT181 GAGAAAGCCA TTAGCATATT CAGGATCCTG GGCAAGAAAA AGTTTGTTTT GTTCTTGGAT241 GATGTGTGGG AGCGGTTTGA TCTCTTAAAG GTGGGGATTC CACTGCCAAA CCAACAAAAT301 AATTCCAAGC TTGTATTCAC GACACGATCT GAAGAGGTTT GTGGCCGCAT GGGAGCTCAC361 AGGCGAATAA AAGTTGAGTG TTTGGCATGG AAGAAAGCCT GGGACTTGTT TCAAAACATG421 GTAGGAGAAG ATACCCTTAA TTCTCATCCT GAAATACCTC AGCCAGCTGA AACTATTGTG481 AAGGAGTGTC TCGGATTGCC ACTAGCGTTG毛葡萄83-4-96(1)1 GGTGTTGGCA AGACAACCTT AGCAAAGTTA GTCTACAATA ATAATGCTGT TGTTGATCAT61 TTCCCTTATT GTGCTTTTGT GTCTTGTAAC TATAAGGGAA GCTCTCGAGG TCATAGCTAA121 ACAAATGGGC AATCCAAGTG CATGGAAAGA AGACAACAGA AGCAATGTAG AAAAAGCAGA181 AGTGGAAGGG TAATGGTCTG AAGAAGGCTC TTGGTCACAA CGAGTGAAGT CGATGCCTTC241 TTTAAAAACT TTCTTGAAGA ATAAGAAGCA TCTTATAGTT TTGGATGACA TAGAAATGGA301 TGCTGGCTTG AATATCTTGC TTAGCAAACT CCCAAAAACA TCAAATGGGA GTCGGATGAT361 CTTGACCACT CGGTATGCGA GGGGACTTGC AGATCTACAG AAGAAGGGCA CTAATCTAAC421 ACTGCAATTA CATGATGATG ATGAGAGTTG GATGCTGTTC AAACATATTT TGAAGGTAGA481 CATACCCCAA GAACTGATAT ATCTTAGAAG AGACATTGTG ACAACATGCT GTGGATTGCC541 ACTAGCGTTG毛葡萄83-4-96(10)1 GGGGTTGGGA AGACAACCTT AGTAAAGTTG GTCTATAACA ATGACATGAT TGTGGATCGT61 TGCCCTTATC GTGCTTAAAC CTTTGTAACT TGTAAATTTC AAATTCTTGA GGACATGATG121 AAAGCATTTC TGGACTACGA CACTAATGAG CTAGTAAGTT GGTGGGATGA GATGACACAG181 ATGGTCAAAG CTTTCATAAG TGATAAGAGG TATCTCATAG TTCTAGATGA TGCCTACCGT241 GTCTTTTGCT TAAATGAAGT CATAGAAGCA TTCCAAGACA CAATGTGTGA GGGTAGAATG301 ATTGAAACTA CATGTGAAAT GAGTTTGCTT TCAAATCTCA AATTGAGGAT TGTTCATCAT361 GCAGTGCGAT TATGAGGAGA TGATGAGAGT CAAGCATTAT TCACGTATGT TGTGAAGGTA421 AACATACCAT TAGAACTACC AAATCTGAGA AGTGAAATTG TGAGAAGATT TAGGGGATTG481 CCACTAGCGT TG毛葡萄83-4-96(11)1 GGTGTTGGGA AGACTACCCT TTTGACCCAA ATCAACAATG AGTTCCTCAA AGAAACTCAT61 GATTTTGATG TTGTGATTTG GGCTGTGGTG TCACGAGATC CAGACTTTCC CAAGGTTCAA121 GACGAAATTG GGAAAAAGGT TGGGTTTTGT GATGGTATAT GGAGAAATAA AAGCAAGGAT181 GAAAAAGCCA TAGACATCTT TAGAGCCTTG CGCAAAAAGA GGTTTGTGTT GTTGTTAGAT241 GACATATGGG AGCCGGTAAA TCTATCAGTC TTGGGAGTTC CAGTTCCAAA TGAAGAAAAT301 AAGTCCAAGC TAGTAATCAC AACCCGATCC GAGGATGTAT GCCGACAAAT GGAAGCTCAA361 ATGAATATCA AAGTGGAGTG CTTGGCATGG CAGGAATCCT AGGATTTGTT TCAGAAGAAG421 GTTGGACAAG ACACTCTTGA CTCCCATGCA GAAATCCCCA TGCTAGCTGA AATAGTTGCC481 AAAGAATGTC GCGGATTGCC ACTAGCGTTG毛葡萄83-4-96(12)1 GGGGTTGGGA AGACTACCCT CATGACCCAA GTCAACAACG AGTTCCTTAA AACTATTCAT61 CAATTTGACA TAGTGATTTG GGTTGTGGTT TCCAGAGACC CAAATCCCGA AAAGGTTCAG121 GATGAAATTT GGAAGAAGGT AGGGTTTTGC GATGATATAT GGAAAAGCAA AAGCCAAGAT181 GAGAAAGCCA TTAGCATATT CAGGATCCTG GGCAAGAAAA AGTTTGTTTT GTTCTTGGAT241 GATGTGTGGG AGCGGTTTGA TCTCTTAAAG GTGGGGATTC CACTGCCAAA CCAACAAAAT301 AATTCCAAGG TTGTATTTAC GACACGATCT GAAGAGGTTT GTGGCCGCAT GGGAGCTCAC361 AGGCGAATAA AAGTTGAGTG TTTGGCATGG AAGCAAGCCT GGGACTTGTT TCAAAACATG421 GTAGGAGAAG ATACCCTTAA TTCTCATCCT GAAATACCTC AGCTAGCTGA AACTATTGTG481 AAGGAGTGTC TCGGATTGCC ACTAGCGTTG毛葡萄83-4-96(14)1 GGGGTTGGTA AGACTACGCT CGCACGGCTT GTTTACAATG ATGATTTGGC CAAAAATTTT61 GAACTAAAAG CGTGGGTTTG TGTGACTGAG GTTTTTTATG TGGAGAATAT AACAAAAGCA121 ATTCTCAATT CGGTGTTAAA TTCTGATGCA AGTGGCTCTT TGGACTTCCA ACAAGTTCAA181 AAGAAATTGA CAGACACTTT AGCAGGGAAA ACTCTTTTTC TCATTCTAGA CGACGTGTGG241 AACGAAAATT ATTGTAATTG GGATCGTTTG CGGGCTCCTT TGAGTGTAGT GGCAAAAGGA301 AGCAAAGTCA TAGTTACAAC ACGCAATAAA AATGTTGCAT TGATGATGGG AGCTGCCGAG361 AATGTTCATG AACTAAACCC CTTATCAGAA GATGCTTGTC GGTCTGTGTT TGAGAAGCAT421 GCATTTGAAC ATAGGAATAT GAAAGATCAT CCAAACTTGG TGTCCATTGG TAGGAAAATT481 GTTGGCAAGT GTGGGGGATT GCCACTAGCG TTG毛葡萄83-4-96(16)1 GGAGTTGGCA AGACTACTTT CGCCAAAGAA GTTTACGACA ACCAGAGAGT GGTGGGACAC61 TTCGATTGCC ATGCTTGGGT CACTGTGTCT CAATCATTCA AGATGGAAGA GCTACTACGG121 AACATGACAA AGAAATTCTA TCAGGGAAGA AAGGAAGCAG TTCCTGAGGG TATCGATACA181 ATAGACGAGA TGTCACTGAT TGCCTTAATC AGGCAGTATT TACAAGATAA AAGGTATGTG241 GTTGTTTTTG ATGATGTATG GAAACTAGAT TTCTGGGGAT TTATTAAATA TGTTTTACCA301 GAAAATGGGA AGGGCAGTAG GATAATAATC ACTACACGTA ATGATGAGGT AGCCTCTTCT361 TGCAAAGAGT CGTCATTTGA TTACATCCAC AAGCTGCAAC CCCTGTCCCC AAAGAGTTCC421 TGGGAACTCT TTTGCAAGAA GACATTCCAG GGAGGATGTC CTCCAGAGTT GGAGAAACTG481 TCTCTTGATA TTGTCAAAAG ATGTGGAGGA TTGCCACTAG CGTTG毛葡萄83-4-96(17)1 GGGGTTGGGA AGACTACCCT TTTGACCCAA ATCAACAATG AGTTCCTCAA AGAAACTCAT61 GATTTTGATG TTGTGATTTG GGCTGTGGTG TCACGAGATC CAGACTTTCC CAAGGTTCAA121 GACGAAATTG GGAAAAAGGT TGGGTTTTGT GATGGTATAT GGAGAAATAA AAGCAAGGAT181 GAAAAAGCCA TAGACATCTT TAGAGCCTTG CGCAAAAAGA GGTTTGTGTT GTTGTTAGAT241 GACATATGGG AGCCGGTAAA TCTATCAGTC TTGGGAGTTC CAGTTCCAAA TGAAGAAAAT301 AAGTCCAAGC TAGTATTCAC AACCTGATCC GAGGATGTAT GCCGACAAAT GGAAGCTTAA361 ATGAATATCA AAGTGGAGTG CTTGGCATGG CAGGAATCCT AGGATTTGTT TCAGAAGAAG421 GTTGGACAAG ACACTCTTGA CTCCCATGCA GAAATCCCCA TGCTAGCTGA AATAGTTGCC481 AAAGAATGTT GCGGATTGCC ACTAGCGTTG毛葡萄83-4-96(18)1 GGTGTGGGGA AAACAACGCT CGCACGGCTT GTTTACAATG ATGATTTGGC CAAAAATTTT61 GAACTAAAAG CGTGGGTTTG TGTGACTGAT GTGTTTGATG TGGAGAATAT AACAAAAGCA121 ATTCTCAATT CGGTGTTAAA TTCTGATGCA AGTGGCTCTT TGGACTTCCA ACAAATTCAA181 AGGAAATTGA CAGACACGTT AGCAGGGAAA ACTCTTTTTC TCATTCTAGA CGACGTGTGG241 AACGAAAATT ATTGTAATTG GGATCGTTTG CGGGCTCCTT TGAGTGTAGT GGCAAAAGGA301 AGCAAAGTCA TAGTTACAAC ACACAAAAAA AATGTTGCAT TGATGATGGG AGCTGCCAAG361 AATGTTCATG AACTAAACCC TTTATCTGAA GATGATTGTT GGTCTGTGTT TGAGAAGCAT421 GCATTTGAAC ATAGAAATAT CAATGATCAT CCGAATCTGG TGTCCATTGG TAGGAAAATT481 GTTGGCAAAT GTGGTGGATT GCCACTAGCG TTG毛葡萄83-4-96(6)1 GGGGTAGGTA AGACAACTCT CGCACGGCAG GTTTACAAGT ATGATTTGGC CAAAAATCTT61 GAACTAAAAG CGTGGGTTTG TGTGACTGAT GTGTTTGATG TGGAGAATAT AACAAAAGCA121 ATTCTCAATT CGGTGTTAGA GTCTGATGCA AGTGGCTCTT TGGACTTCCA ACAAGTTCAA181 AAGAAATTGA CAGACACGTT AGCAGGGAAA ACTTTTTTAC TCGTTCTAGA CGACGTGTGG241 AACGAAAATT GTGGTCATTG GGATCTTTTG CGGGCTCCTT TGAGTGTAGG GTCCAAAGGA301 AGCAAAGTCA TAGTTACAAC ACGCAATAAA AATGTCGCAT TGATGATGGG AGCTGCTAAG361 AATGTTCATG AACTAAACCC TTTATCTGAA GATGCTTGTT GGTCTGTGTT TGAGAAGCAT421 GCATTTGAAC ATAGAGATAT CAATGATCAT CCGAACCTGG TGTCCATTGG TAGGAAAATT481 GTTGGCAAAT GTGGTGGATT GCCACTAGCG TTG毛葡萄83-4-96(4)1 GGGGTGGGGA AGACAACGCT CGCACGGCTT GTTTACAATG ATGATTTGGC CAAAAATTTT61 GAACTAAAAG CGTGGGTTTG TGTGCCTGAT GTGTTTGATG TGGAGAATAT AACAAAAGCA121 ATTCTCAATT CGGTGTTAGA TTCTGATGCA GGCGGCTCTT TGGACTTCCA ACAAGTTCAA181 CGGAAATTGA CAGTCACATT AGCAGGGAAA ACTCTTTTAC TCATTCTAGA TGACGTGCGG241 AACGAAAATT ATTGTAATTG GGATCGTTTG CGGGCTCCTT TGAGTGTAGT GGCAAAAGGA301 AGCAAAGTCA TAGTTACAAC ACGCAAAAAA AATGTTGCAT TGATGATGGG AGCTGCCAAG361 AATGTTCATG AACTAAACCC TTTATCTGAA GATGATTGTT GGTCTGTGTT TGAGAAGCAT421 GCATTTGAAC ATAGAAATAT CAATGATCGT CCGAATCTGG TGTCCATTGG TAGGAAAATT481 GTTGGCAAAT GTGGTGGATT GCCACTAGCG TTG毛葡萄83-4-96(5)1 GGGGTGGGTA AGACTACTCT CGCACGGCAG GTTTACAAGT ATGATTTGGC AAAAAATTTT61 GAACTAAAAG CGTGGGTTTG TGTGACTGAT GTGTTTGATG TGGAGAATAT AACAAAAGCA121 ATTCTCAATT CGGTGTTAGA GTCTGATGCA AGTGGCTCTT TGGACTTCCA ACAAGTTCAA181 AAGAAATTGA CAGACACGTT AGCAGGGAAA ACTTTTTTAC TCGTTCTAGA CGACGTGTGG241 AACGAAAATT GTGGTCATTG GGATCTTTTG CGGGCTCCTT TTAGTGTAGG GTCCAAAGGA301 AGCAAAGTCA TAGTTACAAC ACGCAATAAA AATGTCGCAT TGATGATGGG AGCTGCCAAG361 AATGTTCATG AGCTAAACCC TTTATCTGAA GATGCTTGTT GGTCTGTGTT TCAGAAGCAT421 GCATTTGAAC ATAGAAATAT CAATGATCAT CCGAACCTGG TGTCCATTGG TAGGAAAATT481 GTTGGCAAAT GTGGTGGATT GCCACTAGCG TTG毛葡萄83-4-96(8)1 GGGGTTGGGA AGACAACTTT CGCCAAAGAA GTTTACGACA ACCAGAGAGT GGTGGGACAC61 TTCGATTGCC ATGCTTGGGT CACTGTGTCT CAATCATTCA AGATGGAAGA GCTACTACGG121 AACATGACAA AGAAATTCTA TCAGGGAAGA AAGGAAGCAG TTCCTGAGGG TATCGATACA181 ATAGACGAGA TGTCACTGAT TGCCTTAATC AGGCAGTATT TACAAGATAA AAGGTATGTG241 GTTGTTTTTG ATGATGTATG GAAACTAGAT TTCTGGGGAT TTATTAAATA TGTTTTACCA301 GAAAATGGGA AGGGCAGTAG GATAATAATC ACTACACGTA ATGATGAGGT AGCCTCTTCT361 TGCAAAGAGT CGTCATTTGA TTACATCCAC AAGCTGCAAC CCCTGTCCCC AAAGAGTTCC421 TGGGAACTCT TTTGCAAGAA GACATTCCAG GGAGGATGTC CTCCAGAGTT GGAGAAACTG481 TCTCTTGATA TTGTCAAAAG ATGTGGAGGA TTGCCACTAG CGTTG。