[发明专利]樟芝抗氧化酶的蛋白质与核酸及其制造方法与用途

摘要

本发明涉及樟芝抗氧化酶的蛋白质与核酸，其包含序列辨识编号1、3及5所示的Mn－SOD、1－Cys Prx及2－Cys Prx的氨基酸序列，及可编码该氨基酸序列的核酸。本发明还涉及上述樟芝抗氧化酶的蛋白质与核酸的制造方法及用途。

主权项

1、一种樟芝抗氧化酶的蛋白质，其属于樟芝子实体抗氧化酶的蛋白质，其选自樟芝的超氧歧化酶Mn-SOD、过氧化还原酶1-Cys Prx、过氧化还原酶2-CysPrx：其中，该樟芝的超氧歧化酶Mn-SOD包含(SEQ ID NO：1)所示的氨基酸序列：1 CTATTCACATATAAAAACCACCGTTAACTCTCGCC 36 ATG TCC ATG CTC AGT ACT GCT CGC ACC GCT GCT CGC ACA GCG CGC GCG TCG CGC 1 M S M L S T A R T A A R T A R A S R 90 GCT CTC CCC TTT GCC TCG TTC TCG CTC GCC GCG AGG GCC AAG TCG CTG CAC ACG 19 A L P F A S F S L A A R A K S L H T144 CTC CCT GAG CTT GAC TAC GCG TAC GAT GCA CTC GAG CCG CAC ATC TCT GGC CAG 37 L P E L D Y A Y D A L E P H I S G Q198 ATC ATG AAG CTG CAC CAT ACA AAG CAC CAC CAG ACG TAC GTC ACC GGC CTC AAC 55 I M K L H H T K H H Q T Y V T G L N252 GCG GCG GAG GAG AGC TAC GCC AAG GCG CCC TCG CCC AAG GAG CGG ATT CTC CTT 73 A A E E S Y A K A P S P K E R I L L306 CAG CCC GCG CTC AAA TTC AAT GGC GGA GGT CAC ATC AAT CAC TCG CTC TTC TGG 91 Q P A L K F N G G G H I N H S L F W360 AAG AAC CTT GCG CCC CAG TCG CAG GGT GGC GGC CAG CTC GCT GCG GGT CCC TTC109 K N L A P Q S Q G G G Q L A A G P F414 AAA GAT GCA GTA GAT GCT GAC TTC GGC GGT GTC GAT GGC TTG AAG AAG AAA TTG127 K D A V D A D F G G V D G L K K K L468 AAC GCG GCG ACA GCT GCC ATC CAG GGC TCC GGT TGG GGC TGG CTT GGA TAC AAT145 N A A T A A I Q G S G W G W L G Y N522 CCG ACC ACT AAA AAG CTT GAG GTT GTC ACA ACC GCA AAC CAG GAT CCT CTC CTC163 P T T K K L E V V T T A N Q D P L L576 TCT CAC ATT CCC ATA ATT GGC ATC GAC ATC TGG GAG CAC TCA TAC TAT CTT CAA181 S H I P I I G I D I W E H S Y Y L Q630 TAC CAG AAT GTG AAA GCC GAC TAC CTC CAA GCC ATC TGG AGC GTC ATC AAT TTC199 Y Q N V K A D Y L Q A I W S V I N F684 AAG GAG GCT GAG CGC CGC TAC GTC GAA GCT GCA CAG AAA TAA217 K E A E R R Y V E A A Q K *726 AGTGACGATATAACCTAGAGAGTTCGAACATGCTATTGAGATGACGTGTCAAAGTGGCGA786 CGGAAGAATGGCAGAAGCATGGGAGGACCCATTCGTCGTCGCACTACTGAACTGCATTTG846 TAATATATAACGTTCCAACGACACGCAATTCGTCTCAAAAAAAAAA 891再有，该樟芝的过氧化还原酶1-Cys Prx包含(SEQ ID NO：3)所示的氨基酸序列：1 ACGCGGGGGAGATTAATTTTTCAGCCTTCATCTTCATTGCAGAGACATCATC 53 ATG CCT AGC CTC CGC CTT GGA AGC ATT GCC CCC AAT TTT GAA GCT GAG ACT ACC CAG1 M P S L R L G S I A P N F E A E T T Q110 GGT CAC ATT AAG TTC CAC GAC TGG ATT GGT GAT TCA TGG GCC ATC TTG TTC TCT CAC20G H I K F H D W I G D S W A I L F S H167 CCG GGT GAC TTC ACT CCT GTC TGT ACA ACG GAG CTG GCT GAG GTC GCA CGA AAG GCT39P G D F T P V C T T E L A E V A R K A224 CCT GAA TTC GCA AAG CGC AAT GTC AAA GTT ATC GGT ATC TCT GCC AAC GAT CTC AAT58 P E F A K R N V K V I G I S A N D L N281 GAC CAT GAG AAA TGG GTG CAG GAC ATC AAC GAG TAT GGG ACG AAG TCC CTC GGG CCT77 D H E K W V Q D I N E Y G T K S L G P338 ACG AAC GTC CAG TTT CCA ATT ATA GCA GAC GGG AAC AGG AAG ATC TCA ACC TTG TAT96 T N V Q F P I I A D G N R K I S T L Y395 GAC ATG TTG GAT GAA CAG GAT GCT ACC AAT CGC GAT GCT AAG GGC CTG CCC TTC ACC115 D M L D E Q D A T N R D A K G L P F T452 ATA CGC ACT GTA TTT GTG ATT GAC CCA AAG AAG GTC ATT CGC CTT ACA CTT TCC TAC134 I R T V F V I D P K K V I R L T L S Y509 CCT GCT GCA ACT GGA CGT AAC TTC GAC GAG ATT TTG AGG GTC GTC GAC TCT CTT CAA153 P A A T G R N F D E I L R V V D S L Q566 CTT GGG GAC AAG TAC CGT GTC ACA ACT CCT GTG AAC TGG CAG AAA GGT GAT GAC GTC172 L G D K Y R V T T P V N W Q K G D D V623 ATT GTA CAC CCG TCC GTA AGC AAT GAG GAG GCG AAA ACC TTG TTC CCT GAG GTT ACC191 I V H P S V S N E E A K T L F P E V T680 TTC CAC AAG CAA TCG TAT ATC CGA ACC ACC CCT CTC AAC GTA TAA GTA GAA CAG ATA210 F H K Q S Y I R T T P L N V *737 TGGATTTGTTATCGGATCTGTGTAACTGTATTTGTATAAGTGATGCAATCCTGAAACGTTTCTCATTTCCGCAAA812 AAAAAAAAAAAAAAAAAAAAAAA←837再有，该樟芝的过氧化还原酶2-Cys Prx包含(SEQ ID NO：5)所示的氨基酸序列：1 TTGAATACGACT13 ATG GTC GCC ATC GTC CAG AAA CCT GCG CCC GGA TTC AAG GCC ACG GCC GTC GTT GAT1 M V A I V Q K P A P G F K A T A V V D70 GGT CAG TTC CAG GAT ATT TCT CTA TCT GAC TAC TTC GGA CAA TGG GTG GTT CTC TTC20 G Q F Q D I S L S D Y F G Q W V V L F127 TTC TAT CCC CTT GAC TTC ACT TTC GTA TGC CCA ACT GAA ATC CTT GCC TTC AAT GAC39 F Y P L D F T F V C P T E I L A F N D184 GCC CTT CCT CAA TTC AAG GAG CTG AAC ACT ACT GTC CTC AGC GTC TCA ACT GAT TCA58 A L P Q F K E L N T T V L S V S T D S241 CAC TAC GCG CAT TTG GCA TGG GCC ACG CAA GAC CGC AAG CAG GGT GGT CTT GGT CCG77 H Y A H L A W A T Q D R K Q G G L G P298 AAC CTC AAG CTC CCC ATG ATT GCG GAT AAG AAC ACG CAG ATC TCA CGC GAC TAC GGG96 N L K L P M I A D K N T Q I S R D Y G355 GTT TTG ATC GAG GAA GAG GGT GTT GCT CTT CGC GGT CTC TTC TTG ATT GAC CCC AAG115 V L I E E E G V A L R G L F L I D P K412 GGT ACC CTG AGG CAA ATC ACA ATC AAC GAT TTA CCA GTC GGT CGC TCT GTC GAT GAG134 G T L R Q I T I N D L P V G R S V D E469 ACC ATT CGT CTG ATC AAG GCG TTC CAG TTC ACT GTA TGT CGA TCT ACC GTC ATT TCC153 T I R L I K A F Q F T V C R S T V I S526 ATT GAT ATT AAC ACT GGC AGC CAT ACT GAC CGT TTA AAA GGA CGA ACA TGG TGA GGT172 I D I N T G S H T D R L K G R T W *583 TTGCTGCCAACTGGACAGAAGGCGGCAAGACGATCAAAGCTGATCCCAAGGGTTCATTGGAGTATTTTTCCAC658 AGCGGGTGCAAATGGTCATACAGACGCTAAAGGGACTACCAAGAAGCGTGCTCGCGTTGACTGAGGTTCATCTGT733 TCATTCTTGGAGGCTATAGAAGCAAAGACTATGAGAAGACGTCGAAGACTCAGACTTTGCAGCTATGGTACACCA808 ACATCTTGTGTATCTCACTTTATTTAGACTTTCCCTGTAGATATAGAGTTGACAGACAGACTGCTGCTTGATTGC883 CCAATGCTAAAAACGACGATTCGCCGTCATTAAAAAAAAAAAAAAAAAAAAAAAAAA←939