[发明专利]西瓜隐性核雄性不育基因序列

摘要

本发明公开了一种西瓜隐性核雄性不育基因序列，应用RAPD分子标记技术，采用BSA法对西瓜隐性核不育材料Se18的不育基因进行了分子标记研究。结果表明，引物S1167、S357在不育株DNA中扩增出了多态性特异片段，在可育株DNA中未扩增出多态性片段。验证结果表明，引物S1167在12个不育材料中全部扩增出特异性片段，可以区分不育株与可育株，引物S357在12个不育材料中的8个材料扩增出特异性片段，可以区分不育株与可育株。对特异性片段进行回收、克隆和测序，其结果表明，S1167扩增特异片段有2391个碱基对，而S357扩增的特异片段约有2500个碱基对。经BLAST查询，表明该片段是一种新的西瓜基因DNA序列。

主权项

1.一种西瓜隐性核雄性不育基因序列，其特征在于，该基因序列如下：5’ 10 20 30 40 50ACGTCGCATG CTCCCGGCCG CCATGGCGGC CGCGGGAATT CGATTACCAC 50CCGCTGTGTC AAGTATCATC TAAGGACTTC GATCGCTTCA CTCAAGTCAA 100GATGAACTAT TAAATGAAAC AGCCACTTCG CTTTACAGCC ACGCTTGTTC 150GCCTATCGGC TCACCTCTAA AGCCCCGCTA TACTTCCATT ATGGCCCAGG 200CCCACCCTAT ACAAGGACTT ATTAGGTTCC TGTTCGCGAC TATTGAACAC 250CTATAAGCTC TTTCTTGTCA AGGTTGAAGA CCTGATGAGC ATTAGGCCTC 300TCTCCCTCTG GTTGCGATAG TAAAGCAAAC CCTATTTATT TCACCGGTAG 350GCGAAGGGTC GAAGTCAAGG TTGTGTATTT CACATTAGTG TTGATAATAG 400GGCATTTTCA ATGATTTTCT CGGTATTGTT CCAAAACCAA TCTACTGTGC 450TACGCGACGA AGAACATGTC AAAAAGGTAG TAGTCAACGC GACGAATAGC 500CCGGGGGTGA GCCGATAGGC GAATAAGATG CCGATAGGCT CAGAGGACGA 550AGGAGTAAGT AAGGCGAAGA GGACAAAGTC AAGTAGTTCG TTCCGCTATA 600CCAAAAGGAG GGGCTTTAGA GGCGACTTGA CTTCTCCGCT TCGTGGGACT 650CCGCTCCTAA AGAAGGGGTG CTTTCATTCA AGAAGAGATC CATATCTCTA 700TAGAGCTTCG AAGGGACAGC ACGAAGGTTA CAAGGAAAAA GCCTTGCTAC 750GAGCTCGTAT ATGGATATCC AGCCTTAGTC AAAACCTTAC CTTAATATCG 800TGGATAACGT TGATTCCTCC TTACCTCCCC TTCTCCGCCT AAAATGAAGT 850AAGTGTCTCA ATAGCGAGAA AATCATAGCC CTGGCACTGA AACAAGAGAG 900GAACCTCCTC CAAGACAACG AGAGAGGAAT CAGTCGTTTA CCACTTTCAA 950CTGAATTGCT ACTTTTTTCT TGTTATTGAT AGAGTTGTCT TCCCCGCCCC 1000TGATAAATAC GAGGGGAAAG AAGGATTTCG AAGAAAGGAT TTCGGAGAAA 1050AGAATGGCTG ACACGTTGAC TCGAGACTAT AAGAATTAGA GGACTTTTTT 1100CCCGGTCTGG TGCCAAGCGA GCCGGACCCA AACCGAATAC TCCATAATCA 1150GTAAACAGCA TAGATGCGAA GCATGTTATG CCGCCGCTTA AGGACTCTTC 1200CCAAACGACT CTGAATCTCA AGTATAGAGA GGCTGTTTCA TATAGTTTGA 1250AAAGTGCTTC AGTGACTGAC GATGTTGAAA GTCGTGTATG GCTTTCTAAA 1300GAAGACGACC AACAACTAGC GGCGAACAAG CATAAATGCA ACTCTCATTT 1350CATCATTTCT AGTTCAAATC CCTTATTGCC TAGTTCAACC ACGAACCATC 1400AAAGGTAGTC GGCGAAGATG CGCAGCGGCG AACAGTCCTG GGCCCGAGGT 1450AGGTGCTTTG TTTGTGAGCC GGAAATTCGA AGATTAGTGA TCCCACCGAT 1500TTTGATGTTG GAGTGCTTAA AGGCGTAACC AGTGTTGTCG GAAGTTCTTT 1550TGAAGAAAGA ACTACGGTAC GGGTAATTCT CTAAGGGGCA AGAATCATGA 1600AATGCTCTTT TTTCCAAGGG CGGGTCCTTA TTGGCTCAGC TGCACCAATA 1650ACTGATGCCT ACTGGAAAAG ATTACCTATT AGATTAGTGG ATTAGGCGCA 1700ACTCATAACC TCAGACTATC CTAAGGAGTC GGGTCTCCCA ATCCAATTAA 1750AGAAAGACGG GCAAGACAGA GGAGAAGTAT GCCGGTACCA CTTACCTTCC 1800CCGTTCCCGG GGTTGGGAGT TTTTTTCAAA TATCGGGCAG CTGAATCTTA 1850TAGCTTGAGC TATTCTCCTT AAGCTCCGAG GACGAATGGA GGTGAGCCGA 1900TAGGCGAACA GTACAGAGAG AGGGTGCTTA GTGATAAGCA CCACATACTA 1950AC1AATCCTG CGAAGAAGAC TTGCAACAAA GAAAATCTCT GGAAAGGGAT 2000TACCAGCTCC CCTAAAGCCG TGATGATTGG CTCGATCCTA ACCGGTGAAG 2050AAAGAAGGTT TACCGCTAGG CGAACTAGCA TAAGGCCGTA AGCCTTGGTA 2100AGCTAAGGGG GAGCTTTCAA CTGATGGAGA AACGTACTTT TCTTTATTCA 2150TCTCTATTGG TATTGGAAAG TGTTCAGTCT ACGGCATCCC CGTAAGCAAA 2200GGCGGACCTA ATCTTATCTT TCCTCTATTC CCGATATAGC TATAGCACGG 2250TGGAGTCGGC TTCTTTATCC GTGGTCGTGG TGGGGCGTAC TCTCAATCTA 2300AGCGGGTGGT AATCACTAGT GAATTCGCGG CCGCCTGCAG GTCGACCATA 2350TGGGAGAGCT CCCAATCGCG TTGATGCGTA GCCTGTTTCG T。