[发明专利]斑节对虾热休克蛋白90的基因的核苷酸及氨基酸序列

摘要

本发明从近源物种肌动蛋白的基因的CDS序列的保守区设计的兼并引物，从斑节对虾的性腺中提取总RNA，并用PCR法扩增，克隆到斑节对虾热休克蛋白90基因的表达序列。斑节对虾热休克蛋白90基因的分离，对于研究软体动物体内的热休克蛋白90对环境中的污染因素的应激反应，从而探讨能否将该分子作为一种生物学指标具有重要的理论意义和实践意义。

主权项

1、一种斑节对虾热休克蛋白90基因，其特征在于具有以下RNA核苷酸及相应的氨基酸序列：caacacattccaaagccaacaactttttgttcctttgtcggtcaaagcttcacacattcc 60aaaatg cct gag gag gtc acc acc ggg gag gag gag gtc ttc gcg ttc cag 111 Met Phe Glu Glu Val Thr Thr Gly Glu Glu Glu Val Phe Ala Phe Gln 16 1 7 13gcg gag atc gcg cag ctg atg tcc ctg atc atc aac acc ttc tac agc 159Ala Glu Ile Ala Gln Leu Met Ser Leu Ile Ile Asn Thr Phe Tyr Ser 3217 23 29aac aag gag atc ttc ctg cga gag ctg atc tcg aac tcg tcc gac gcc 207Asn Lys Glu Ile Phe Leu Arg Glu Leu Ile Ser Asn Ser Ser Asp Ala 4833 39 45ctc gac aag atc cgc tac gag tcg ctg acg gac ccg tcc aag ctg gag 255Leu Asp Lys Ile Arg Tyr Glu Ser Leu Thr Asp Pro Ser Lys Leu Glu 6449 55 61agc ggc aag gac ctg ttc atc aag ctg gtg ccc aac aag gac gac cgc 303Ser Gly Lys Asp Leu Phe Ile Lys Leu Val Pro Asn Lys Asp Asp Arg 8065 71 77acg ctc acc atc atc gac agt ggc atc ggc atg acc aag gcc gac ctg 351Thr Leu Thr Ile Ile Asp Ser Gly Ile Gly Met Thr Lys Ala Asp Leu 9681 87 93gtg aac aac ctg ggc acc atc gcc aag tcg ggc aca aag gcc ttc atg 399Val Asn Asn Leu Gly Thr Ile Ala Lys Ser Gly Thr Lys Ala Phe Met 11297 103 109gag gcg ctg cag gcg ggc gcc gac atc tcg atg atc ggc cag ttc ggc 447Glu Ala Leu Gln Ala Gly Ala Asp Ile Ser Met Ile Gly Gln Phe Gly 128113 119 125gtg ggc ttc tac tcc gcg tac ctg gtg gcc gac aag gtg acc gta gtg 495Val Gly Phe Tyr Ser Ala Tyr Leu Val Ala Asp Lys Val Thr Val Val 144129 135 141tcg agg aac aac gac gac gaa cag tac atc tgg gag tcg tcg gcg ggc 543Ser Arg Asn Asn Asp Asp Glu Gln Tyr Ile Trp Glu Ser Ser Ala Gly 160145 151 157ggg tcg ttc acg gtg cgc cac gac acc ggt gaa ccc atc ggc cgt ggt 591Gly Ser Phe Thr Val Arg His Asp Thr Gly Glu Pro Ile Gly Arg Gly 176161 167 173aca aag atc acc ctc cac ctg aag gag gac cag aca gag tac ctc gag 639Thr Lys Ile Thr Leu His Leu Lys Glu Asp Gln Thr Glu Tyr Leu Glu 192177 183 189gag cgt cgc gtg aag gag atc gtg aag aag cac tcg caa ttc att ggc 687Glu Arg Arg Val Lys Glu Ile Val Lys Lys His Ser Gln Phe Ile Gly 208193 199 205tat ccc atc aag ctc ctc gtc gag aag gag agg gac aag gaa gtg tct 735Tyr Pro Ile Lys Leu Leu Val Glu Lys Glu Arg Asp Lys Glu Val Ser 224209 215 221gac gat gag gaa gag gag aaa gaa gag aag gaa gag gaa gca gag gag 783Asp Asp Glu Glu Glu Glu Lys Glu Glu Lys Glu Glu Glu Ala Glu Glu 240225 231 237gac aag ccc aaa atc gaa gat gta ggc gag gac gaa gaa gcc gac aaa 831Asp Lys Pro Lys Ile Glu Asp Val Gly Glu Asp Glu Glu Ala Asp Lys 256241 247 253gag aag ggc gaa gac aag aag aaa aag aag acg gtg aag gag aag tac 879Glu Lys Gly Glu Asp Lys Lys Lys Lys Lys Thr Val Lys Glu Lys Tyr 272257 263 269acg gag gac gaa gag ctg aac aag acg aag ccc ctg tgg acg cgc aac 927Thr Glu Asp Glu Glu Leu Asn Lys Thr Lys Pro Leu Trp Thr Arg Asn 288273 279 285ccc gac gac atc tcg aag gag gag tac ggc gag ttc tac aag tcg ctg 975Pro Asp Asp Ile Ser Lys Glu Glu Tyr Gly Glu Phe Tyr Lys Ser Leu 304289 295 301acc aac gac tgg gag gac cac ctg gcc gtg aag cac ttc agc gtg gag 1023Thr Asn Asp Trp Glu Asp His Leu Ala Val Lys His Phe Ser Val Glu 320305 311 317ggc cag ctt gag ttc cgc gcc ctc ctg ttc ctg ccg cgc cgc gcc ccc 1071Gly Gln Leu Glu Phe Arg Ala Leu Leu Phe Leu Pro Arg Arg Ala Pro 336321 327 333ttc gac ctg ttc gag aac cgc aag cag aag aac aag atc aag ctg tac 1119Phe Asp Leu Phe Glu Asn Arg Lys Gln Lys Asn Lys Ile Lys Leu Tyr 352337 343 349gtg cgt cgc gtg ttc atc atg gag aac tgc gag gaa ctg atc ccc gag 1167Val Arg Arg Val Phe Ile Met Glu Asn Cys Glu Glu Leu Ile Pro Glu 368353 359 365tac ctg aac ttc atc aac ggt gtc gtc gac tcc gag gat ctg cct ctc 1215Tyr Leu Asn Phe Ile Asn Gly Val Val Asp Ser Glu Asp Leu Pro Leu 384369 375 381aac atc tct cgt gag atg ctg caa cag aac aag atc ctg aaa gtt atc 1263Asn Ile Ser Arg Glu Met Leu Gln Gln Asn Lys Ile Leu Lys Val Ile 400385 391 397agg aag aat ctc gtc aag aag acc ctc gaa ctt ttt gaa gaa att gtt 1311Arg Lys Asn Leu Val Lys Lys Thr Leu Glu Leu Phe Glu Glu Ile Val 416401 407 413gac gac aag gaa agc tac aag aag ttc tac gaa aac ttc tcc aag aac 1359Asp Asp Lys Glu Ser Tyr Lys Lys Phe Tyr Glu Asn Phe Ser Lys Asn 432417 423 429ctc aaa ctc gga atc cac gag gat tcc acc aac cgc aag aag ctt gcc 1407Leu Lys Leu Gly Ile His Glu Asp Ser Thr Asn Arg Lys Lys Leu Ala 448433 439 445gaa ttc ctg agg tac cac act tct gcc tct ggc gac gaa atg tcc tcc 1455Glu Phe Leu Arg Tyr His Thr Ser Ala Ser Gly Asp Glu Met Ser Ser 464449 455 461ctc aag gag tac gtg tcc cgc atg aag gag aac cag aag cac atc tac 1503Leu Lys Glu Tyr Val Ser Arg Met Lys Glu Asn Gln Lys His Ile Tyr 480465 471 477ttc atc act ggc gag act cgc gaa cag gtg cag aac tct gcc ttc gtg 1551Phe Ile Thr Gly Glu Thr Arg Glu Gln Val Gln Asn Ser Ala Phe Val 496481 487 493gag agg gtg aag aag cgc ggc ttc gag gtc atc tac atg acc gaa ccc 1599Glu Arg Val Lys Lys Arg Gly Phe Glu Val Ile Tyr Met Thr Glu Pro 512497 503 509atc gac gaa tac tgc gtt cag cag ctg aag gaa tac gac ggg aag cag 1647Ile Asp Glu Tyr Cys Val Gln Gln Leu Lys Glu Tyr Asp Gly Lys Gln 528513 519 525ctt gtc tcg gtg acg aag gaa ggc ctt gaa ctc ccc gag gac gag gag 1696Leu Val Ser Val Thr Lys Glu Gly Leu Glu Leu Pro Glu Asp Glu Glu 544529 535 541gag aaa aag aag ttc gag gaa cag aag acc aag ttc gag aac ctg tgc 1743Glu Lys Lys Lys Phe Glu Glu Gln Lys Thr Lys Phe Glu Asn Leu Cys 560545 551 557aag gta atg aag gac att ttg gac aag cgc gtt gag aag gtg gtg gtg 1791Lys Val Met Lys Asp Ile Leu Asp Lys Arg Val Glu Lys Val Val Val 576561 567 573agc aac cgg ctg gtg acc tct ccg tgc tgc atc gtg acc tcc cag tac 1839Ser Asn Arg Leu Val Thr Ser Pro Cys Cys Ile Val Thr Ser Gln Tyr 592577 583 589ggc tgg acc gcc aac atg gag cgc atc atg aag gct cag gcg ctg agg 1887Gly Trp Thr Ala Asn Met Glu Arg Ile Met Lys Ala Gln Ala Leu Arg 608593 599 605gac acc tcg acc atg ggc tac atg gcc gcc aag aag cac ctt gag atc 1935Asp Thr Ser Thr Met Gly Tyr Met Ala Ala Lys Lys His Leu Glu Ile 624609 615 621aac ccc gac cac agc atc atc gaa acc ctg aga caa aag gcg gat gcc 1983Asn Pro Asp His Ser Ile Ile Glu Thr Leu Arg Gln Lys Ala Asp Ala 640625 631 637gac aag aac gac aag tct gtg aag gat ctg gtg atg ctg ctg ttc gag 2031Asp Lys Asn Asp Lys Ser Val Lys Asp Leu Val Met Leu Leu Phe Glu 656641 647 653agc tcc ctt ctg tcg tct ggc ttc agc ctg gag gac cca ggt gtc cac 2079Ser Ser Leu Leu Ser Ser Gly Phe Ser Leu Glu Asp Pro Gly Val His 672657 663 669gcc agc cgc atc tac aga atg atc aag ctt ggc ctg ggt att gac gag 2127Ala Ser Arg Ile Tyr Arg Met Ile Lys Leu Gly Leu Gly Ile Asp Glu 688673 679 685gag gac gcc ccg atg gag gag gcc gag acc ttg gag gag gat atg ccc 2175Glu Asp Ala Pro Met Glu Glu Ala Glu Thr Leu Glu Glu Asp Met Pro 704689 695 701ccc ctc gaa ggt gat gac gag gac gcc tct cgc atg gaa gaa gtc gat 2223Pro Leu Glu Gly Asp Asp Glu Asp Ala Ser Arg Met Glu Glu Val Asp 720705 711 717taa 2226atattcgtcacaacttaaaatattcacccattatataccaaagctaatcattgtcattca 2286ttcgggaaccaaaatacttctctaatcttggtatattttggcttctctggctttcatcat 2346tccgatcacgcccaacattccataagatttaaacaagcattagttttagttatagacaaa 2406gatatattctgttataaggatttattctttcgtttatgtaaataatttgtaacaactttg 2466ttacaataaaactcgagaccatttaaaaaaaaaaaaaaaaaaaaaaaa 2514