[发明专利]黄鳍鲷肌动蛋白的基因序列

摘要

本发明从近源物种肌动蛋白的基因的CDS序列的保守区设计的兼并引物，从黄鳍鲷的脾脏中提取总RNA，并用PCR法扩增，克隆到黄鳍鲷肌动蛋白的基因的表达序列。该基因序列的获得不但使通过体外重组技术获得具有免疫活性的重组蛋白成为可能，而且为进一步探讨其功能提供了理论基础。

主权项

1、一种黄鳍鲷肌动蛋白基因，其特征在于具有以下RNA核苷酸及相应的氨基酸序列：atg gat gat gaa atc gcc gca ctg gtt gtt gac aac gga tcc ggt atg 48Met Asp Asp Glu Ile Ala Ala Leu Val Val Asp Asn Gly Ser Gly Met 161 7 13tgc aaa gcc ggt ttc gcc gga gac gac gcc cct cgt gct gtc ttc ccc 96Cys Lys Ala Gly Phe Ala Gly Asp Asp Ala Pro Arg Ala Val Phe Pro 3217 23 29tcc atc gtc ggt cgc ccc agg cat cag ggt gtg atg gtg ggt atg ggc 144Ser Ile Val Gly Arg Pro Arg His Gln Gly Val Met Val Gly Met Gly 4833 39 45cag aag gac agc tac gtt ggt gat gag gcc cag agc aag aga ggt atc 192Gln Lys Asp Ser Tyr Val Gly Asp Glu Ala Gln Ser Lys Arg Gly Ile 6449 55 61ctg acc ctg aag tac ccc atc gag cac ggt att gtg acc aac tgg gat 240Leu Thr Leu Lys Tyr Pro Ile Glu His Gly Ile Val Thr Asn Trp Asp 8065 71 77gac atg gag aag atc tgg cat cac acc ttc tac aac gag ctg aga gtt 288Asp Met Glu Lys Ile Trp His His Thr Phe Tyr Asn Glu Leu Arg Val 9681 87 93gcc cct gag gag cac cct gtc ctg ctc aca gag gcc ccc ctg aac ccc 336Ala Pro Lys Lys His Pro Val Leu Leu Thr Glu Ala Pro Leu Asn Pro 11297 103 109aaa gcc aac agg gag aag atg acc cag atc atg ttc gag acc ttc aac 384Lys Ala Asn Arg Glu Lys Met Thr Gln Ile Met Phe Glu Thr Phe Asn 128113 119 125acc cct gcc atg tac gtt gcc atc cag gct gtg ctg tcc ctg tat gcc 432Thr Pro Ala Met Tyr Val Ala Ile Gln Ala Val Leu Ser Leu Tyr Ala 144129 135 141tct ggt cgt acc act ggt atc gtc atg gac tcc ggt gat ggt gtg acc 480Ser Gly Arg Thr Thr Gly Ile Val Met Asp Ser Gly Asp Gly Val Thr 160145 151 157cac aca gtg ccc atc tat gag ggc tat gcc ctg ccc cac gcc atc ctg 528His Thr Val Pro Ile Tyr Glu Gly Tyr Ala Leu Pro His Ala Ile Leu 176161 167 173cgt ctg gac ttg gcc ggc cgc gac ctc aca gac tac ctc atg aag atc 576Arg Leu Asp Leu Ala Gly Arg Asp Leu Thr Asp Tyr Leu Met Lys Ile 192177 183 189ctg aca gag cgt ggc tac tcc ttc acc acc aca gcc gag agg gaa atc 624Leu Thr Glu Arg Gly Tyr Ser Phe Thr Thr Thr Ala Glu Arg Glu Ile 208193 199 205gtg cgt gac atc aag gag aag ctg tgc tat gtc gcc ctg gac ttc gag 672Val Arg Asp Ile Lys Glu Lys Leu Cys Tyr Val Ala Leu Asp Phe Glu 224209 215 221cag gag atg ggc act gct gcc tcc tcc tcc tcc ctg gag aag agc tat 720Gln Glu Met Gly Thr Ala Ala Ser Ser Ser Ser Lys Glu Lys Ser Tyr 240225 231 237gag ctg cct gac gga cag gtc atc acc atc ggc aat gag agg ttc cgt 768Glu Leu Pro Asp Gly Gln Val Ile Thr Ile Gly Asn Glu Arg Phe Arg 256241 247 253tgc cca gag gcc ctc ttc cag cca tcc ttc ctc ggt atg gag tcc tgc 816Cys Pro Glu Ala Leu Phe Gln Pro Ser Phe Leu Gly Met Glu Ser Cys 272257 263 269gga atc cat gag acc acc tac aac agc atc atg aag tgt gat gtc gac 864Gly Ile His Glu Thr Thr Tyr Asn Ser Ile Met Lys Cys Asp Val Asp 288273 279 285atc cgt aag gac ctg tat gcc aac aca gtg ctg tct gga ggt acc acc 912Ile Arg Lys Asp Leu Tyr Ala Asn Thr Val Leu Ser Gly Gly Thr Thr 304289 295 301atg tac cct ggc atc gct gac agg atg cag aag gag atc aca gcc ctg 960Met Tyr Pro Gly Ile Ala Asp Arg Met Gln Lys Glu Ile Thr Ala Leu 320305 311 317gcc cca tcc acc atg aag att aag atc att gcc cca cct gag cgt aaa 1008Ala Pro Ser Thr Met Lys Ile Lys Ile Ile Ala Pro Pro Glu Arg Lys 336321 327 333tac tct gtc tgg atc gga ggc tcc atc ctg gcc tcc ctg tcc acc ttc 1056Tyr Ser Val Trp Ile Gly Gly Ser Ile Leu Ala Ser Leu Ser Thr Phe 352337 343 349cag cag atg tgg atc agc aag cag gag tac gat gag tcc ggc ccc tcc 1104Gln Gln Met Trp Ile Ser Lys Gln Glu Tyr Asp Glu Ser Gly Pro Ser 368353 359 365atc gtc cac cgc aaa tgc ttc 1125Ile Val His Arg Lys Cys Phe 375369 375