[发明专利]携带HIV基因的腺病毒载体疫苗

摘要

本发明描述了一种腺病毒载体，它携带密码子优化的HIV结构基因和/或HIV调控和辅助基因，以及异源启动子和转录终止子。当单独或者联合质粒载体疫苗采用初次－加强免疫的方案时，该病毒载体疫苗可以用于预防HIV感染。

主权项

1.一种携带HIV基因的腺病毒载体疫苗，它包含经优化的HIV结构蛋白gag和pol以及HIV调控蛋白rev、tat的多核苷酸序列或其片段或衍生物，相关核苷酸序列如下：含有优化密码子的HIV1gag序列：atgggcgcca gggcctccat cctgaggggc ggcaagctgg acaagtggga gaagatcagg 60ctgaggcctg gcggcaagaa gcactacatg ctgaagcatc tggtctgggc ctccagggag 120ctggagaggt ttgccctgaa ccctggcctg ctggagacct ctgagggctg caagcagatc 180atcaagcagc tgcagcctgc cctgcagaca ggcacagagg agctgaggtc cctgttcaac 240acagtggcca ccctgtactg tgtgcatgct gagattgagg tgagggacac caaggaggcc 300ctggacaaga ttgaggagga gcagaacaag atccagcaga agacccagca ggccaaggag 360gctgatgaga aggtctccca gaactacccc attgtgcaga acctgcaggg ccagatggtg 420catcagcccc tgtcccccag gaccctgaat gcctgggtga aggtggtgga ggagaaggcc 480ttctcccctg aggtgatccc catgttcaca gccctgtctg agggcgccac cccccaggac 540ctgaacacca tgctgaacac agtgggcggc catcaggctg ccatgcagat gctgaaggac 600accatcaatg aggaggctgc tgagtgggac aggctgcatc ctgtgcatgc tggccctgtg 660gcccctggcc agatgaggga gcccaggggc tctgacattg ctggcaccac ctccaccctg 720caggagcaga ttggctggat gaccaacaac cctcccatcc ctgtgggcga gatctacaag 780aggtggatca tcctgggcct gaacaagatt gtgaggatgt actcccccac ctccatcctg 840gacatcaagc agggccccaa ggagccattc agggactatg tggacaggtt cttcaagacc 900ctgagggctg agcaggccac ccaggatgtg aagaactgga tgacagacac cctgctggtg 960cagaatgcca accctgactg caagaccatc ctgagggccc tgggccctgg cgcctccctg 1020gaggagatga tgacagcctg ccagggcgtg ggcggcccat cccacaaggc cagggtgctg 1080gctgaggcca tgtcccagac caacaacacc atcctgatgc agaggtccaa cttcaagggc 1140tccaagagga ttgtgaagtg cttcaactgt ggcaaggagg gccacattgc caagaactgc 1200agggccccca ggaagaaggg ctgctggaag tgtggcaagg agggccatca gatgaaggac 1260tgcacagaga ggcaggccaa cttcctgggc aagatctggc catcccacaa gggcaggcct 1320ggcaacttcc tgcagtccag gcctgagccc acagcccccc ctgctgagtc cttcaggttt 1380gaggagacca cccctgcccc caagcaggag cccaaggaca gggagcccct gacctccctg 1440aggtccctgt ttggctctga ccccctgtcc cagtaaa 1477；含有优化密码子的HIV1pol序列：atggccccca tctcccccat tgagacagtg cctgtgaagc tgaagcctgg catggatggc 60cccaaggtga agcagtggcc cctgacagag gagaagatca aggccctgac agccatctgt 120gatgagatgg agaaggaggg caagatcacc aagattggcc ctgacaaccc atacaacacc 180cccatctttg ccatcaggaa gaaggactcc tccaagtgga ggaagctggt ggacttcagg 240gagctgaaca agaggaccca ggacttctgg gaggtgcagc tgggcatccc ccatcctgct 300ggcctgaaga agaagaagtc tgtgacagtg ctggctgtgg gcgatgccta cttctctgtg 360cccctggaca aggacttcag gaagtacaca gccttcacca tcccctctgt gaacaatgag 420acccctggca tcaggtacca gtacaatgtg ctgccccagg gctggaaggg ctcccctgcc 480atcttccagt gctccatgac caagatcctg gagccattca ggaagcagaa ccctgacatt 540gtgatctacc agtacatggc tgccctgtat gtgggctctg acctggagat tggccagcac 600aggaccaaga ttgaggagct gagggagcat ctgctgaagt ggggcttcac cacccctgac 660aagaagcatc agaaggagcc cccattcctg tggatgggct atgagctgca tcctgacaag 720tggacagtgc agcccatcca gctgcctgag aaggactcct ggacagtgaa tgacatccag 780aagctggtgg gcaagctgaa ctgggcctcc cagatctacc ctggcatcaa agtgaggcag 840ctgtgcaagc tgctgagggg cgccaaggcc ctgacagaca ttgtgcccct gacagaggag 900gctgagctgg agctggctga gaacagggag atcctgaagg agcctgtgca tggcgcctac 960tatgacccat ccaaggagct gattgctgag atccagaagc agggccagga ccagtggacc 1020taccagatct accaggagcc attcaagaac ctgaagacag gcaagtatgc caagatgagg 1080acagcccaca ccaatgatgt gaagcagctg acagaggctg tgcagaagat tgccatggag 1140tccattgtga tctggggcaa gatccccaag ttcaggctgc ccatccagaa ggagacctgg 1200gagacctggt ggacagacta ctggcaggcc acctggatac ctgagtggga gtttgtgaac 1260accccccccc tggtgaagct gtggtaccag ctggagaagg accccattgc tggcgtggag 1320accttctatg tggctggcgc tgccaacagg gagaccaaga ttggcaaggc tggctatgtg 1380acagacaggg gcaggaagaa gattgtctcc ctgacagaca ccaccaacca gaagacagcc 1440ctgcaggcca tctacattgc cctgcaggac tctggctctg aggtgaacat tgtgacagcc 1500tcccagtatg ccctgggcat catccaggcc cagcctgaca agtctgagtc tgagctggtg 1560aaccagatca ttgagcagct gatcaagaag gagagggtct acctgtcctg ggtgcctgcc 1620cacaagggca ttggcggcaa tgagcaggtg gacaagctgg tctccaatgg catcaggaag 1680gtgctgttcc tggatggcat tgacaaggcc caggaggagc atgagaagta ccactccaac 1740tggagggcca tggcctctga cttcaacctg ccccccattg tggccaagga gattgtggcc 1800tcctgtgacc agtgccagct gaagggcgag gccatgcatg gccaggtgga ctgctcccct 1860ggcatctggc agctggcctg cacccatctg gagggcaaga tcatcctggt ggctgtgcat 1920gtggcctctg gctacattga ggctgaggtg atccctgctg agacaggcca ggagacagcc 1980tacttcatcc tgaagctggc tggcaggtgg cctgtgaagg tgatccacac agccaatggc 2040tccaacttca cctctgctgc tgtgaaggct gcctgctggt gggctggcat ccagcaggag 2100tttggcatcc catacaaccc ccagtcccag ggcgtggtgg cctccatgaa caaggagctg 2160aagaagctga ttggccaagt gagggaccag gctgagcatc tgaagacagc tgtgcagatg 2220gctgtcttca tccacaactt caagaggaag ggcggcattg gcggctactc tgctggcgag 2280aggattgtgg acatcattgc cacagacatc cagaccaggg agctgcagaa gcagatcatc 2340aagatccaga acttcagggt ctactacagg gactccaggg accccatctg gaagggccct 2400gccaagctgc tgtggaaggg cgagggcgct gtggtgatcc aggacaactc tgacatcaag 2460gtggtgccca ggaggaaggc caagatcatc aaggactatg gcaagcagat ggctggcgct 2520gactgtgtgg ctggcaggca ggatgaggac taaa 2554；含有优化密码子的HIV1rev序列：atggctggca ggtctggcga ctctgatgag gccctgctga gggctgtgag gatcatcaag 60atcctgtacc agtccaaccc ataccctgag cccaggggca ccaggcaggc caggaagaac 120aggaggagga ggtggagggc caggcagaac cagatccact ccatctctga gaggattctg 180tccacctgcc tgggcaggcc cacagagcct gtgcccctgc agctgccccc cctggagagg 240ctgcacatca cagactctga gaggggcggc acctctggca cccagcagcc ccagggcacc 300acagagggcg tgggctcccc ccagatcctg gtggagtccc ctgctgtgct ggagtctggc 360accaaggagt aaa 373：含有优化密码子的HIV1tat序列：atggagcctg tggaccccaa cctggagcca tggaaccatc ctggctccca gcctgagaca 60gcctgcaaca actgctactg caagaggtgc tcctaccact gcctggtctg cttccagaag 120aagggcctgg gcatctccta tggcaggaag aagaggaggc agaggaggtc tgccccccag 180tcctctgagg accatcagaa cctgatctcc aagcagtccc tgcccaggac ccagggcgac 240caggctggct ctgaggagtc caagaagaag gtggagtcca agaccaagcc tgacccattt 300gactaaa 307。