[发明专利]日本血吸虫中国大陆株酪氨酸激酶TK4保守区基因及其克隆表达方法和应用

摘要

本发明涉及日本血吸虫(中国大陆株)酪氨酸激酶TK4保守区(SjcTK4)基因的序列及其克隆表达方法和应用。本发明公开的SjcTK4基因具有序列1所示的核苷酸序列及编码相应的氨基酸序列。本发明还公开了SjcTK4基因在大肠杆菌中的表达方法。经制备纯化重组蛋白和DNA疫苗，免疫C₅₇BL/6小鼠，发现本发明SjcTK4基因可用于制备防治日本血吸虫病的重组蛋白疫苗和DNA疫苗。

主权项

1. 一种编码日本血吸虫中国大陆株酪氨酸激酶TK4保守区基因的核苷酸序列，其特征在于该核苷酸序列及其编码的相应氨基酸序列如下：tta ggt gga gga aat ttt ggt cat gtt gtc aaa ggc tta tat cgt aca 48Leu Gly Gly Gly Asn Phe Gly His Val Val Lys Gly Leu Tyr Arg Thr1 5 10 15cct tca ggt caa gaa gta cct gtt gca gtt aaa gca ttg aaa cca aat 96Pro Ser Gly Gln Glu Val Pro Val Ala Val Lys Ala Leu Lys Pro Asn20 25 30caa att gtc aat tct ggt gaa cga gaa atc ctt gct gaa gca cgt aca 144Gln Ile Val Asn Ser Gly Glu Arg Glu Ile Leu Ala Glu Ala Arg Thr35 40 45atg gct caa ttg aag cat cgg cat ata gta aga tta atc ggt gta tgt 192Met Ala Gln Leu Lys His Arg His Ile Val Arg Leu Ile Gly Val Cys50 55 60aaa gaa gag caa ttt atg ctt gtc ctt gaa ttg gcg cca tta ggt cca 240Lys Glu Glu Gln Phe Met Leu Val Leu Glu Leu Ala Pro Leu Gly Pro65 70 75 80att aat aaa tat tta aaa aaa cgc cct gat gtc agt gtg cat aca cta 288Ile Asn Lys Tyr Leu Lys Lys Arg Pro Asp Val Ser Val His Thr Leu85 90 95act gaa tta atg cat caa gtt gca tta ggt atg gcc tat tta gaa tca 336Thr Glu Leu Met His Gln Val Ala Leu Gly Met Ala Tyr Leu Glu Ser100 105 110tgt aaa ttt gtt cat cgt gac tta gct gct cgt aat gtt cta cta gtt 384Cys Lys Phe Val His Arg Asp Leu Ala Ala Arg Asn Val Leu Leu Val115 120 125aca cgt cat ttt gct aag att agt gat ttt gga atg agt aaa gcg tta 432Thr Arg His Phe Ala Lys Ile Ser Asp Phe Gly Met Ser Lys Ala Leu130 135 140aat ttc ggt agt gat tat tat cgg gca gcg act gct ggg aaa tgg cct 480Asn Phe Gly Ser Asp Tyr Tyr Arg Ala Ala Thr Ala Gly Lys Trp Pro145 150 155 160tta aaa tgg tat gca cca gaa tgc ata tat tat ttt cga ttt gat tca 528Leu Lys Trp Tyr Ala Pro Glu Cys Ile Tyr Tyr Phe Arg Phe Asp Ser165 170 175aaa tct gac gta tgg agt tat ggt ata act tta tgg gaa gtt tac tcc 576Lys Ser Asp Val Trp Ser Tyr Gly Ile Thr Leu Trp Glu Val Tyr Ser180 185 190tat ggt 582。Tyr Gly