[发明专利]多花黄精微卫星标记

摘要

本发明公开了多花黄精微卫星标记，包括多花黄精微卫星(CT)n富集文库的构建；含微卫星序列阳性克隆的筛选及测序，得到55个含有微卫星重复序列的克隆；筛选得到14个多态性高的微卫星分子标记Pc1、Pc2、Pc3、Pc4、Pc5、Pc6、Pc7、Pc8、Pc9、Pc10、Pc11、Pc12、Pc13、Pc14；本发明14个微卫星标记能用于研究多花黄精的遗传多样性，黄精属植物种间和居群间的变异和进化关系及多花黄精品种的鉴定，具有重复性好的特点，是一种可靠有效的分子标记。

主权项

1、多花黄精微卫星标记，其特征在于：所述微卫星标记的标号分别为：Pc1、Pc2、Pc3、Pc4、Pc5、Pc6、Pc7、Pc8、Pc9、Pc10、Pc11、Pc12、Pc13、Pc14；其核苷酸序列分别为：Pc1：gatcgggcct cccttttctc cacgaacaag aaagagaaaa agagggaggt aaagaaaaat 60aaaggagaag gagagaagac aagtctgacc ccttctcaag ttgataaaga aagggggaga 120gaagagagaa ttagagagga gagtagacaa gctacctgat cctctcggcg gagatcaaga 180aggaaagaag gaaatgagag aaatggagag gagataaccc tctcctatcg gcagcaacta 240agaacagaga aggagaaaga gagagaaagt ggggggggga gagagagaga gagagagaga 300gagagagaga gagagagaga gagagagaga gagagagaga gaaatgaatc atctctccct 360ctcggctggg gatcaaaaga gagggaatga ttgaggggga gaaagaaaat tctccccccc 420cctcactcaa caattaatgc ggcaaggaca tgcatggtgt aaggatggag gaagtgaaga 480Pc2：gaccgccctt gataagaaaa tcgtatagcg ataagaagta ctcgcttcct tcttgagggt 60gttgatgata aaggcccaaa gccaatatct ttcaaaaaag cccaagctca agtcttcatt 120ttttgctcct tggcttgtga ggatgaaagc ctaaggcttt ggaatgagct cttgctctac 180ctccttgctt cttctctttt cttcttgttg ctcttgcttg caaatgagag agagagagag 240agagagagag agagagagag agagagagag agagagagag agagagagag agagagagag 300agagagagag agagagagag agggagatgg ggtttggtga catgtgaggt aaggttaagt 360agagggaaag gtgtgactga tgccatgtgg caatgattga atccataagg attatgatga 420agctaaatgg ctaaagaaga tggttttaag gagatgatgc caagtgacct tgaatgtgta 480gagattgagg ggatgttgcc atgtgttgga tgatggctaa atgatatgga atatctcatc 540caacggttga 550Pc3：gatcacccct tgaatcactc tctactcact ccaatcaaaa ccctctcatc catttgtctc 60actctcattt ttcctcttcc attctcaaga gagctagcat gacctaagca agagagagag 120agagagagag atagagagag agagagagag agagagagag agagagagag agagagagag 180agagagagag agagagagag agagagagag aggagcaaga acaagagagt gaaggagcaa 240gagaaggaga attatgcctt gaacatttaa agcctttggc tttggaaagc ttcatcaaca 300ccctcaagaa 310Pc4：gatctgcaaa agctctaggg agctgaactc aagcttgaag cttagagagg aggaaatgga 60gatgagtgat gcaaggtagg agaggagctc tgccttctct tcttgctggt tcaagagaga 120gagagagaga gagagagaga gagagagaga gagagggaga gagagagaga gagagagaga 180gagagagaga gagagagaga tgtggtgttg ggctcaataa atgaaggggg aaggctcatt 240aaatgcaagg gggctgacaa gacataaagt tggctcaatg ccatgtctca tttataagaa 300atgagacaag caccagatga atcaacagat tcaaggga 338Pc5：gatcagaaga agaatttcaa gaagattgtt tgtttgcaat taagcttaaa tttcctacaa 60tttctccttc ttattcttct tgcttacatc ttgattggac acccgaagaa atacaagagg 120atgtaccgat gtctccgaag agagagagag agagagagag agagagagag agagagagag 180agagagagag agagagagag agagagagag agagagagac gttagttcta gtactaccac 240aagagatagt cactttgaga ggaatccccc ggaggatgtg aaggaggcaa ttgggccaaa 300gaaagatttg gaagttattg aagagtttgg tgccaaagaa gaagaaaaag gaaactaaga 360ctatggccaa gcactactag aaaatctgca tataacatcg cctctaagaa atcagtttaa 420tatgcgcctg atgtaatagg cgtacgagac attgattgtg aaaataaccg atgttatgta 480tcatttataa catcagttgc taaattaatc gattttaaaa gtaaaattaa aaaactacgg 540gaaccccaaa aggggaaaaa acaagaagcg ctaaaaagtt cccccctttt ttatttactt 600agccgctcct tctcccctct cctctctcag 630Pc6：gagctccccc tcggctgcta atagacagag aaaaagagat agagaaaaaa atgagaaaat 60gagagaaaga gagagataga gagagggagg acctctcggc tgccagtagc aagacaagag 120agagagggaa tgagtgttta tgacatataa ttggaagggt ggcatgctat ctcccctcac 180ttgcattaaa tgcagtctca tcacattaat gaggagagag agagagagag agagagagag 240agagagagag agagagagag agagagagag agagagagag agagagagag agagagagag 300agagagagac agacagacag acagacagag acatacaaga atgagacgaa tatagaacta 360attgagggaa aaccctaata ttagataatt tagcagaatg agggtccggc accagataat 420caaatacaat aatataggaa taaataaaca gtacccctca tatacgtgca taaatacgta 480taccgggaac tgaaggttac aatcctaccc aacttataaa aaacttgtcc ttaaaatttc 540gataaagagg atagacgtaa caagaagatc aagcatgcaa gataatgcaa gaacgataac 600tatgatggag tgaacaagcg ataaatcaaa aagaaaagaa caactaccag actacggaac 660aaatgagggg tacttctgac ggatctgatc atctaactcc catgttgaat ctcctagtga 720ctgcatatcc actgcaccct gaccactttt gacaatcata tccctga 767Pc7：aatcacaaac gtgcaaacaa ttgcacatcc cctctcttgc ccccgagaat ttgcaaaatt 60ccatccgtat cccctctcgc cttcttcctc ccgattccgt tgcccccttt ataaatgcgc 120cctttacttc agttgacaaa cctctccctt tcccaatccc gttccgttac gtttaaatta 180gcggtcgctc tctccctccc tccctctctc tctctctctc tccctctctc tctctctctc 240tctctctctc tctctctcta tatatataaa gtctctgatc gaaatctagg gtttcgattc 300cgattgctct gggttttcgt ttttctcgtt ccggggttgg gattagggct tccaacgccg 360gaggagttgg ggttagggtt ttgcgactac gagatgttgg ggacgagcct tcagtttggg 420cgggggaacg gcgac 435Pc8：gatcttgtat tccaagctct ctcaagctga aatcatctat cagcctgtga gcatcttttg 60taattttgtc ccagttcccg gtatacgtat ttgtgcacat atatatgggg tactgtatat 120ttatttctgt attattgcct ttgattatct ggtatcggac cctaatttac cggaatattg 180gggttttact tcaataattc tcatttctct ctctctctct ctctctctct ctctctctct 240ctctctctct ctttctctct ctccattaat gtgatgagat tgcatttaat gcaagtaagg 300gggagagagc atgacaccct ctaatcatat gacataaacc ctcattctct ccctctctct 360tgtcctccct agcaaccaag agtctttttc ccactccctt tctctactat tctctcattt 420cctctttctc tgtcactctc tccttctctt tctcattctc agagcaagca cctgaggggg 480gtgattaatt tcttccccct cctttcattt gcatcacctt actccctaat ccatcatgag 540ggaacttctt gagttccttc ctctttgtca agcaacttta gaag 584Pc9：gatcttgttt tggaccggga tagggcaata tatcgccaag tggccgatat atcactcaag 60atatatcggt gaaaatctcg gccgatatct aatgctatca ggcttttttc ttttaatttt 120tcaaaccagt taagtcttca gacccgtcaa cccgtacctg atacgttaat atataatgtt 180aagagacgag ttagggttcg ctatcgagcc cctctctctc tctctctctc tctctctctc 240tctctctctc tctctctcac ggcgatcagg ttagggttcg aaattaatga aaattcttga 300aattgatcga caaaaagggg tcaattagac atgggttatt caattcgagg tggactttga 360ttgaatctgg gaaaaagtta agagcttggc aaggatttg 399Pc10：gatctagttg ttgtctttaa taaattgaat gaactcggac attaagtcta ggtaagtgga 60aattaatcac aacccaacaa caagctataa aagcttagaa aatgaacatg aagttcagag 120agtcatggag agcctcagga ttgagagtcc aagagctcac tgctcaaaga gcaagaaaag 180ggcactcgcc tgagaagtga cagcaaagct taaaagagct agaatctcaa gctttaagct 240tagagagaga actccaatag agggagagct tcaagggaag aagaagctcc aagctttctc 300ttgctcttgc tggtgagaga gagagagaga gagagagaga gagagagaga gagagagaga 360gagagagaga gagagagaga gaggtgagca attatgaagg ggagggggtc acattaaatg 420aggagttgct caaggaggag agagggggat gactagataa gggcatgtct ctaataaata 480gaaacaagac aaaaaggaaa acagatgaat catcagactc aagcaagagg aacggtaata 540agcacagtta actccacaga gaggattaca agcagggaaa ttcaatacag aatag 595Pc11：gatctctagc tccaaagctt ggtccttttc ctcttcttgc tgctcttctt cttctttgct 60tgcttgagca aaaaggagag atagaatgag atggatgaag agaatgggag agggtggaga 120agtgttggag ttaaacatgg aagagagaga gagagagaga gagagagaga gagagagaga 180gagagagaga gagagagaga gagagagaga gagagagaga gagagagaga gagagagaga 240gatgcattaa atgcaagcat ggggatgatg gttgttttct tggaatggga gaagtgtgtt 300gaagtgaaag tccattacat gacaaaagag atgtcatacc aaatgaatgg ttaggattaa 360gttgttttgg atggtgagat tggtttgcaa gaataagatt caaaatggac atatgattta 420agttgcaatg aatcagaaaa caatataata cgtgcgaagc acatattaca agatgtgggg 480gttacatttt tcgcaaacac ttgagcgaaa agctctgtcc cgagaggact cagccttcgg 540ccttgtggcg ggacttcgct tgtggagaaa ttacctttgt agacttctcc tctagagaag 600ctcccttctg gcggatcttg gctctggcga gattcctttg ctgcggatct cgcctctggc 660gggattccct cttgtgg 677Pc12：gatcaagaca ccaggaaatg aggtcatcag tgaaagcatc atgtagccag aagacactgt 60ttatatcaac ttttcatttt cctctttgca taggaattat tttaattgtc ttctttaacg 120cacgcgcacc cagaccgaga aacacaccac atacacacaa tatatacaca tgcagacata 180ctgacgcgtg cacacacgcg cgaggagaga gagagagaga gagagagaga gagagagaga 240gagagagaga gagagagaga gagagagaga gacctcagca caggccagga acatttttgc 300gagagcctgt ttcgttgagc ttgagacaga ctttgtaagc ccaaagtcga gcaaaattgg 360gcggtgtggg tgttccttgc ctacaagaaa atttcctaca tgaaagataa accgaaaatg 420aatccccaac tcaaagttgt acgatcacta atgtgaaaat acactttagt atactctcga 480agaacggcca aaagcaagca taccaggatg ag 512Pc13：gattaagggt tctctaatta gagttcccaa ggagagtttc aagcttaagg aagctgagaa 60agaagaggca ttcgcctagg ttgatctgca aaagctctag ggagctgaac tcaagcatga 120agcttagagt ggaagaaatg gagtagagat caaagggttg aagaagagga agttctgcct 180tcacttcaag ctggttcaag agagagagag agagggagag agagagagag agagagagag 240agagagagag agagagagag agagagagag agagagagag agagagagag agagagatga 300ggagttgggc tcaataaatg agggggactc attaattgag agaaggggct gacaagacat 360aaagttagcc ttaccatatc tcatttatga gaaatgagac aagcaccaga tgaatcaaca 420gattcaaggg agatctctct atattagata caggtaaagc tacagagagg aattcaaact 480agggttat 488Pc14：gatctttgct ctcggcctgg tgaaggaaaa agagagagaa ttaggggaaa ggaaacatgg 60gggatatttg gcaatgattt gaggggagag catggcatct cccctccaac attaaatgct 120ctctatactc atttaatgaa gggggggagg gagagagaga gagagagaga gagagagaga 180gagagagaga gagagagaga gagagagaga gagagagaga gagagagaga gagagagaga 240gaaacacaat aatggaagag aattctccta ctagcagata ttaaagcaga tagtgggccc 300aacactatat aaatacctgc aactctacag aataaacaca cagtacccca tatatacgta 360cacaaatacg tatactggga accggaggtt acactttctt tactatgccc cgctggtatg 420ccttttctca tgcctttaca aacatgtagg cataatttct aggatagagt gga 473。