[发明专利]七十四个大鼠源miRNA及其相应的miRNA前体和反义寡核苷酸

摘要

本发明提供了七十四个与大鼠坐骨神经离断过程相关的miRNA序列、其前体序列和反义寡核苷酸序列，以及其在制备治疗周围神经损伤药物中的用途。

主权项

七十四个大鼠源miRNA，其特征在于包含如下任一miRNA序列：(1)miRNA sx89 5′UGUCUGGGAGCAGCCAAGGACA 染色体定位 16p16(2)miRNA sx90 5′AUGCCACCUUUCGCGUUGUGG 染色体定位12q12(3)miRNA sx91 5′UUUGGAACCUCCUCCCUAGGGC 染色体定位13q13(4)miRNA sx92 5′CUCGGCGCCCCACACUGAGCUU 染色体定位17p13(5)miRNA sx93 5′AACGAAUCGCAGGCACCUCACA 染色体定位2q11(6)miRNA sx94 5′ACAGGGAUGCAGACUGUGAAGA 染色体定位3q22(7)miRNA sx95 5′UAAAGGGGGCCAGGCUCUGAGC 染色体定位3q24(8)miRNA sx96 5′UAGGGAGCUUCGGGCUGACAGC 染色体定位5q21(9)miRNA sx97 5′AGCAGGGAUACAGGACUGGGUAA 染色体定位7q34(10)miRNA sx98 5′UGUAGGAAAGGAGUGUGGAGA 染色体定位9q21(11)miRNA sx99 5′ACAGAGUGUGUGUGUCUGUGUGG 染色体定位10q12(12)miRNA sx100 5′AGUCCAGGGCUGAGUCAGCGGA 染色体定位2q44(13)miRNA sx101 5′UACACAUGGUACAGAAGGAAGA 染色体定位11q12(14)miRNA sx102 5′AGGUGCUGUGGAGAGGUCUGC 染色体定位11q22(15)miRNA sx103 5′UCAUUCUCGCUGCUCUGGAGU 染色体定位17p12(16)miRNA sx104 5′UCUGGCAGUUGGAGAUCAUUUUU 染色体定位1q12(17)miRNA sx105 5′UGACGGACAGGACGGAGGGAG 染色体定位1p12(18)miRNA sx106 5′UGGGAAGGAUAGUUUGAUUGUGG 染色体定位3p12(19)miRNA sx163 5′CAGACUGAGCACUGGGCAGAAA 染色体定位8q24(20)miRNA sx108 5′UGGUUGGGCCCUGGAAGAAC 染色体定位10q12(21)miRNA sx109 5′UGAUGUGUUUAUAUCCAGUUGG 染色体定位14q22(22)miRNA sx110 5′GAGUAAGAAGGAAGAUUUAGC 染色体定位15q23(23)miRNA sx111 5′CUAGCCUUGCUGGCUGACUGGAG 染色体定位19p11(24)miRNA sx112 5′GGGAGGAUGGUGGGGACAGAGG 染色体定位1q36(25)miRNA sx113 5′UGGAAAGUUGAGAGCAGCUCG 染色体定位2q44(26)miRNA sx114 5′AUUCAGAUGGGCAGAUUGGGA 染色体定位4q22(27)miRNA sx115 5′ UCCACAGAGGCAUAGGCAGAAC 染色体定位4q22(28)miRNA sx116 5′UCUGGGAGUGGAGUAGAUGGGA 染色体定位5q22(29)miRNA sx117 5′ AGAGGGAUUCUGGGUACAGAAGCA 染色体定位5q22(30)miRNA sx118 5′ACAGGGGCAUGAGGCUAAGACAG 染色体定位8q24(31)miRNA sx119 5′GCAGCACCCCCUAGACCCCCGG 染色体定位7q36(32)miRNA sx120 5′ UUGGAGGAAAGAAUGACUGAGG 染色体定位14q21(33)miRNA sx121 5′ GAGAGAGAGGGAGCAAGGGAG 染色体定位4q22(34)miRNA sx122 5′GAAGAGGGCGUUGGGUUUAUGUGA 染色体定位12q15(35)miRNA sx123 5′AUAAGUGUAGAGAGUCUGUAGU 染色体定位1q31(36)miRNA sx124 5′ AGGGAGAGAUAAGGGGACACUGU 染色体定位11q11(37)miRNA sx125 5′UAGCCAGGGGCUUGACUGCAAG 染色体定位1q22(38)miRNA sx126 5′UGUGUUUUGUGUAUGUACAUG 染色体定位17q12.3(39)miRNA sx127 5′UGAGGUGACCUGAGAACCUAAGG 染色体定位17p14(40)miRNA sx128 5′UGCUCUGUCCAGAGUUAGGGA 染色体定位18p12(41)miRNA sx129 5′UCACGGGCACAGGAAGCUGAUC 染色体定位1q41(42)miRNA sx130 5′UCACGGGCACAGGAAGCUGAUC 染色体定位1q41(43)miRNA sx131 5′ UUAUGUGAAGUUGGGAUGAGU 染色体定位4q42(44)miRNA sx132 5′UCUGAUUAUAUACAUACGUGC 染色体定位3q21(45)miRNA sx133 5′UUGACAGGCUGUGAACUGCAGGC 染色体定位5q36(46)miRNA sx134 5′CUUUGGUGGCUUAGUUCUUUGUG 染色体定位6q32(47)miRNA sx135 5′ UACAGUUAGACGUAGAGACCAU 染色体定位3q42(48)miRNA sx136 5′UCCUGGCAGGGGACACGAGGACA 染色体定位1q55(49)miRNA sx137 5′UGGGGUAGCUUGGGAAUUUGAC 染色体定位1q43(50)miRNA sx138 5′ UGACUUUCCAGAGAACCCUUGA 染色体定位xq21(51)miRNA sx139 5′AUGUAAGUGUGUAUGUAUAUG 染色体定位1q55(52)miRNA sx140 5′UAAGUGUGUAUGUAUAUGUGU 染色体定位18q12.1(53)miRNA sx141 5′UGUGUUUUGUGUGUGUACAUGUG 染色体定位17q12.3(54)miRNA sx142 5′UGUGUUUUGUGUGUGUACAUGUG 染色体定位17q12.3(55)miRNA sx143 5′UGUGUUUUGUGUGUGUACAUGUG 染色体定位17q12.3(56)miRNA sx144 5′ UGUGUUUUGUGUGUGUACAUGUG 染色体定位17q12.3(57)miRNA sx145 5′UGUGUUUUGUGUGUGUACAUGUG 染色体定位17q12.3(58)miRNA sx146 5′UGUGUUUUGUGUGUGUACAUGUG 染色体定位17q12.3(59)miRNA sx147 5′ GGUGACAGUAAGCUGUAGUUGC 染色体定位7q36(60)miRNA sx148 5′AUGCCACCUUUCGCGUUGUGGA 染色体定位12q12(61)miRNA sx149 5′UGGUCUAGACUGUGGAGAGCUGU 染色体定位8q21(62)miRNA sx150 5′ACUGGGCUCUGAGAUUCGGCUAC 染色体定位9q35(63)miRNA sx151 5′ ACUAGACGGUCUGUGCUCUCCA 染色体定位11q23(64)miRNA sx152 5′ CGGGCGGAACGCGGAGACCUGGC 染色体定位16q11(65)miRNA sx153 5′UUGGGAGGAGCAGCUGAGGGAU 染色体定位2q34(66)miRNA sx154 5′AAAGGUUGAGGACUCCUUGGGC 染色体定位3p13(67)miRNA sx155 5′UCUCCCAGCUGUGACUGACAAGC 染色体定位3q41(68) miRNA sx164 5′GUGGGAGAGCAGUGAGGGGCA 染色体定位9q12(69)miRNA sx157 5′AGGGACGGUGGCUCGGUUGUGA 染色体定位10q24(70)miRNA sx158 5′AUAAGACCAAGAACUGUGCAAAG 染色体定位15p12(71)miRNA sx159 5′ UAAGGGAGGGAGAGAAUGAGUGU 染色体定位1q51(72)miRNA sx160 5′ UGACGGACAGGACGGAGGGAGGGC 染色体定位1p12(73)miRNA sx161 5′ UCCUGGCAGGGGACACGAGGAC 染色体定位1q55(74)miRNA sx162 5′GUGAGGGGACACGUCAUGCUGGGG 染色体定位8q24。