[发明专利]提高稻瘟病抗性的水稻OsMOS4基因及其应用

摘要

本发明公开了一种提高稻瘟病抗性的水稻OsMOS4基因，能够对水稻OsMOS4基因进行过量表达和RNAi沉默表达，利用农杆菌转化获得转基因水稻。通过转基因植株对稻瘟病菌抗性测定及其水杨酸含量分析，结果表明OsMOS4过量表达植株叶片内的水杨酸含量显著多于对照组，而RNAi转基因株系则低于对照组；此外，稻瘟病菌接种过量表达植株可增强水稻抗病能力，表明OsMOS4基因可参与水稻的抗病性调控，而且可能是水稻SAR途径的关键基因，该基因的表达可促进水稻抗病能力提高，具有较好的开发前景。

主权项

一种提高稻瘟病抗性的水稻OsMOS4基因，其特点在于，该OsMOS4水稻基因表达的核糖核酸分子序列信息计算机可读版本是，核苷酸序列：ATGGCGGCGGAGGCCGCGGCGGAGATCGTGCGGGAGATCGCGGCGGTGGGCGCCGCCGATTTGGCTGCGGCCGCCGAGCCGCTGCGCGCGGACTGCCTCCGCCTCGCTCGGAAGGTCTCGCTGCTGTCGCACCTCGTCGCGGAGGTCGCCGAGGCGGGGGCCGGGGGTGGGGACGCGGACGCGGCGGATTCGTGGTTGGGGGATCTGGTCAGGGCGCTGCAGGCCGCCAGGAGGTTCGTCGAGCTCGGGCGCGCGCCGGCGCGACCGTCGAGGGCGTCGGATCAGGATGCCGTCTGCAACAATGTTGCTGTTCAGTTCAAGTTTGTGACTTGGCAGTTGCAAACAGTTCTAGCAAGACTTCCACAGAGCTGTTTTCAAATATCAGATGAAGTTCAAGAAGAGGTTGATTTAGTGCGAGCTCAGCTTCGAAGAGAAATGGAGAAGAAAGGAGACATTGATGTAAACATTTTTTCAAAATTCCATGATATCTTAGCTCTTCATGTTAGTACTGTTGGATCGCAGTCTGAGCAATCACATGGCCAGCCAGACACACCACAGATGGAAAACTTATGCAACGGTCATCTGGAGTTGCAAAATATCATTATGCTAGTTTCAGAAATAAGTGGGGTGCCCAAGTCTGACGCAGAGAGAATAACTTCTCAGCTAATTGAAGGACTTGAAAACATGAGAGTTACAGATTCTAAAAAACCTGTCAGTGTTAGCCAATCAAGTGATGAAACAAAAGCATCTCCTGAGACACATAAGAAGTCTGACGCCGTGGCTATTCCTGAAGATTTTCGATGCCCAATATCTCTTGAGCTGATGAGAGATCCTGTCATTGTTTCCACAGGACAGACCTACGAGCGTGCCTTCATCCAAAGGTGGATAGACTGTGGAAACCGGACCTGTCCTAAAACTCAACTGAAGCTACAAAACATTACTCTGACCCCAAATTATGTTCTGCGAAGTTTGATATTGCAGTGGTGTGAGGAAAAAGGGATCGAACCACCTACCAGATCTAAGAACGATGGTGCATATCTTGAGGTTGGTGGGGAGAGAGTAGCTATTGAAACATTAGTTCGTAATCTCTCTAGTAGCTCATTAGATGAACGGAAATCGGCTGCTGCCGAAATAAGATCTTTGGCCAAGAAAAGCACTGACAATCGTATACTTCTAGCGGAATCTGGTGCAATATCCGCTCTAGTGAAACTTTTGTCCTCCAAAGACCTGAAAACCCAAGAACATGCAGTTACAGCTCTTCTGAATCTCTCCATATATGATCAGAACAAGGAACTGATAGTAGTTGCGGGTGCCATTGTCCCAATCATACAGGTGTTGAGGAAGGGGGGCATGGAGGCAAGAGAAAATGCAGCTGCAGCTATTTTCAGCTTGTCACTTATTGATGATAACAAGATAACTATTGGAAGCACTCCAGGGGCAATTGAAGCATTAGTTGAGTTGCTGCAGAGTGGCAGTCCAAGGGGTAGAAAAGATGCAGCAACAGCACTGTTCAACTTATGCATATACCAAGCAAACAAGGTCCGTGCAGTTCGAGCAGGAATCCTCGCACCACTGATTCAGATGCTGCAGGATTCATCCAGAAATGGAGCTATTGACGAAGCACTAACAATCTTGTCAGTTCTTGTGAGCCACCATGAGTGTAAAATTGCCATAGCGAAGGCTCATGCTATACCCTTTTTGATAGACTTGTTGAGGTCAAGTCAGGCCCGTAACAAGGAGAATGCTGCAGCCATCTTGCTTGCCCTTTGCAAGAAGGATGCTGAGAACCTCGCTTGTATAGGGAGGTTGGGTGCTCAAATACCACTAACTGAGCTGTCCAAGACCGGCACAGACAGAGCCAAGCGCAAGGCAACCTCTCTCCTGGAGCATCTCAGTAAGTTGCAGGTGCTCTAA氨基酸序列：MAAEAAAEIVREIAAVGAADLAAAAEPLRADCLRLARKVSLLSHLVAEVAEAGAGGGDADAADSWLGDLVRALQAARRFVELGRAPARPSRASDQDAVCNNVAVQFKFVTWQLQTVLARLPQSCFQISDEVQEEVDLVRAQLRREMEKKGDIDVNIFSKFHDILALHVSTVGSQSEQSHGQPDTPQMENLCNGHLELQNIIMLVSEISGVPKSDAERITSQLIEGLENMRVTDSKKPVSVSQSSDETKASPETHKKSDAVAIPEDFRCPISLELMRDPVIVSTGQTYERAFIQRWIDCGNRTCPKTQLKLQNITLTPNYVLRSLILQWCEEKGIEPPTRSKNDGAYLEVGGERVAIETLVRNLSSSSLDERKSAAAEIRSLAKKSTDNRILLAESGAISALVKLLSSKDLKTQEHAVTALLNLSIYDQNKELIVVAGAIVPIIQVLRKGGMEARENAAAAIFSLSLIDDNKITIGSTPGAIEALVELLQSGSPRGRKDAATALFNLCIYQANKVRAVRAGILAPLIQMLQDSSRNGAIDEALTILSVLVSHHECKIAIAKAHAIPFLIDLLRSSQARNKENAAAILLALCKKDAENLACIGRLGAQIPLTELSKTGTDRAKRKATSLLEHLSKLQVL。