[发明专利]一种稳定、抗干扰能力强的血清磷脂检测试剂及检测方法

摘要

本发明涉及血清磷脂检测技术领域，特别涉及一种血清磷脂检测试剂，试剂R1中含有缓冲液，磷脂酶D，DAOS，抗坏血酸氧化酶，胆红素氧化酶，聚乙二醇6000，蔗糖，黄原胶，甘露醇，海藻糖，BSA，聚氧丙烯氧化乙烯甘油醚（Pluranic L64），防腐剂；试剂R2中含有缓冲液，4‑氨基氨替吡啉，过氧化物酶，胆碱氧化酶，聚乙二醇6000，蔗糖，黄原胶，甘露醇，海藻糖，BSA，聚氧丙烯氧化乙烯甘油醚（Pluranic L64），防腐剂。采用HEPES缓冲液及Trinder反应新色原DAOS，添加多种稳定剂，显著改善了试剂的稳定性；通过添加胆红素氧化酶和抗坏血酸氧化酶，可以有效避免胆红素和抗坏血酸的干扰，大大增强试剂的抗干扰能力。此外，优选的新型非离子表面活性剂聚氧丙烯氧化乙烯甘油醚（Pluranic L64）的加入可防止反应体系浑浊，增强底物的稳定性，提高试剂的抗干扰能力。

主权项

1.一种血清磷脂检测试剂，其特征在于包括试剂R1和试剂R2，所述试剂R1和试剂R2的组成如下：试剂R1的组分为：缓冲液··········································.........··100mmol/L，磷脂酶D·················································1KU/L，N‑乙基‑N‑(2‑羟基‑3‑磺丙基)‑3'5‑二甲氧基苯胺钠盐·········1.4mmol/L，抗坏血酸氧化酶··········································2KU/L，胆红素氧化酶············································2KU/L，聚乙二醇6000············································10g/L，蔗糖····················································20g/L，黄原胶··················································0.5g/L，甘露醇··················································20g/L，海藻糖··················································10g/L，牛血清白蛋白············································2g/L，聚氧丙烯氧化乙烯甘油醚··································1g/L，防腐剂··················································0.5g/L；2）试剂R2的组分为：缓冲液··················································100mmol/L，4‑氨基氨替吡啉··········································2.5mmol/L，过氧化物酶··············································20KU/L，胆碱氧化酶··············································8KU/L，聚乙二醇6000············································10g/L，蔗糖····················································20g/L，黄原胶··················································0.5g/L，甘露醇··················································20g/L，海藻糖··················································10g/L，牛血清白蛋白············································2g/L，聚氧丙烯氧化乙烯甘油醚··································1g/L，防腐剂··················································0.5g/L；所述试剂R1中的缓冲液和试剂R2中的缓冲液均为25℃，pH为7.2的HEPES缓冲液；所述试剂R1中的防腐剂和试剂R2中的防腐剂均为NaN3；所述试剂R1与试剂R2的比例为4：1。