[发明专利]通过组织培养获得灰毡毛忍冬再生植株的方法及其培养基

摘要

本发明公开了通过组织培养获得灰毡毛忍冬再生植株的方法及其培养基。本发提供了一种用于获得灰毡毛忍冬再生植株的培养基，由下述质量份的营养成分组成：大量元素以NH4NO3计1650份、微量元素以KI计0.83份、铁盐以FeSO4·7H2O计37.3份、有机成分以烟酸计0.5份、玉米素0.1份‑1.5份、吲哚丁酸0.1份‑0.5份和蔗糖30000份。本发明所提供的用于获得灰毡毛忍冬再生植株的分化培养基针对性强，适用性好。本发明所提供的通过组织培养获得灰毡毛忍冬再生植株的方法，具有不受地区、气候与季节限制，便于大规模生产具有优良性状的单株。

主权项

1.一种获得灰毡毛忍冬再生植株的方法，包括如下步骤：将灰毡毛忍冬外植体接种到愈伤诱导培养基上进行诱导培养，得到灰毡毛忍冬愈伤组织；将灰毡毛忍冬愈伤组织接种到分化培养基上进行分化培养，得到灰毡毛忍冬再生芽；将所述灰毡毛忍冬再生芽接种到生根培养基上进行生根培养，即得到灰毡毛忍冬再生植株；所述灰毡毛忍冬外植体为灰毡毛忍冬的新生嫩叶或灰毡毛忍冬新生茎段；所述愈伤诱导培养基由以下成分组成：NH4NO3、KNO3、KH2PO4、CaCl2·2H2O、MgSO4·7H2O、KI、Na2MoO4·2H2O、H3BO3、CuSO4·5H2O、MnSO4·H2O、CoCl2·6H2O、ZnSO4·7H2O、Na2EDTA·2H2O、FeSO4·7H2O、烟酸、肌醇、盐酸硫胺素、盐酸吡哆醇、甘氨酸、6‑苄基腺嘌呤、激动素、吲哚丁酸、蔗糖、琼脂和水；以上成分在所述愈伤诱导培养基中浓度分别为：NH4NO3 1.65g/L、KNO3 1.90g/L、KH2PO4 0.17g/L、CaCl2·2H2O 0.44g/L、MgSO4·7H2O 0.37g/L、KI 0.83mg/L、Na2MoO4·2H2O 0.25mg/L、H3BO3 6.2mg/L、CuSO4·5H2O 0.025mg/L、MnSO4·H2O 16.9mg/L、CoCl2·6H2O 0.025mg/L、ZnSO4·7H2O 8.6mg/L、Na2EDTA·2H2O 27.8mg/L、FeSO4·7H2O 37.3mg/L、烟酸0.5mg/L、肌醇100mg/L、盐酸硫胺素0.1mg/L、盐酸吡哆醇0.5mg/L、甘氨酸2.0mg/L、6‑苄基腺嘌呤3.0mg/L、激动素1.0mg/L、吲哚丁酸0.2mg/L、蔗糖30g/L和琼脂7g/L；所述诱导培养基的pH值为6.0；所述分化培养基由以下成分组成：NH4NO3、KNO3、KH2PO4、CaCl2·2H2O、MgSO4·7H2O、KI、Na2MoO4·2H2O、H3BO3、CuSO4·5H2O、MnSO4·H2O、CoCl2·6H2O、ZnSO4·7H2O、Na2EDTA·2H2O、FeSO4·7H2O、烟酸、肌醇、盐酸硫胺素、盐酸吡哆醇、甘氨酸、玉米素、吲哚丁酸、蔗糖、琼脂和水；以上成分在所述分化培养基中浓度分别为：NH4NO3 1.65g/L、KNO3 1.90g/L、KH2PO4 0.17g/L、CaCl2·2H2O 0.44g/L、MgSO4·7H2O 0.37g/L、KI 0.83mg/L、Na2MoO4·2H2O 0.25mg/L、H3BO3 6.2mg/L、CuSO4·5H2O 0.025mg/L、MnSO4·H2O 16.9mg/L、CoCl2·6H2O 0.025mg/L、ZnSO4·7H2O 8.6mg/L、Na2EDTA·2H2O 27.8mg/L、FeSO4·7H2O 37.3mg/L、烟酸0.5mg/L、肌醇100mg/L、盐酸硫胺素0.1mg/L、盐酸吡哆醇0.5mg/L、甘氨酸2.0mg/L、玉米素0.1mg/L‑1.5mg/L、吲哚丁酸0.1mg/L‑0.5mg/L、蔗糖30g/L和琼脂6g/L‑8g/L；所述分化培养基的pH值为5.8‑6.2；所述生根培养基由以下成分组成：NH4NO3、KNO3、KH2PO4、CaCl2·2H2O、MgSO4·7H2O、KI、Na2MoO4·2H2O、H3BO3、CuSO4·5H2O、MnSO4·H2O、CoCl2·6H2O、ZnSO4·7H2O、Na2EDTA·2H2O、FeSO4·7H2O、烟酸、肌醇、盐酸硫胺素、盐酸吡哆醇、甘氨酸、吲哚丁酸、蔗糖、琼脂和水；以上成分在所述生根培养基中浓度分别为：NH4NO3 0.825g/L、KNO3 0.95g/L、KH2PO4 0.085g/L、CaCl2·2H2O 0.22g/L、MgSO4·7H2O 0.185g/L、KI 0.83mg/L、Na2MoO4·2H2O 0.25mg/L、H3BO3 6.2mg/L、CuSO4·5H2O 0.025mg/L、MnSO4·H2O 16.9mg/L、CoCl2·6H2O 0.025mg/L、ZnSO4·7H2O 8.6mg/L、Na2EDTA·2H2O 27.8mg/L、FeSO4·7H2O 37.3mg/L、烟酸0.5mg/L、肌醇100mg/L、盐酸硫胺素0.1mg/L、盐酸吡哆醇0.5mg/L、甘氨酸2.0mg/L、吲哚丁酸0.2mg/L‑1.0mg/L、蔗糖10g/L和琼脂5g/L‑8g/L；所述生根培养基的pH值为5.8‑6.2。