[发明专利]免疫细胞培养基与其应用

摘要

本发明公开了一种免疫细胞培养基，其包含无水氯化钙等多种无机盐、L‑亮氨酸等多种氨基酸、白介素2、白介素4等组分。本发明的免疫细胞培养基在培养CAR‑T细胞时，能够显著提高细胞的增殖倍数，大幅延长细胞培养天数，并能够有效的保持表达表面标志物，同时其还具有保质期长，易于制备等优点。

主权项

1.一种免疫细胞培养基，其特征在于包括：无水氯化钙112.5mg/L～115.8mg/L、L‑亮氨酸57.85mg/L～58.95mg/L、五水硫酸铜0.0011mg/L～0.0012mg/L、L‑赖氨酸盐酸盐92.20mg/L～93.25mg/L、硫辛酸0.101mg/L～0.108mg/L、九水硝酸铁0.04mg/L～0.05mg/L、L‑蛋氨酸16.98mg/L～17.28mg/L、酚红7.8mg/L～8.3mg/L、七水硫酸亚铁0.410mg/L～0.418mg/L、L‑苯丙氨酸35.42mg/L～36.55mg/L、1,4‑丁二胺二盐酸盐0.078mg/L～0.080mg/L、氯化钾310.5mg/L～311.3mg/L、L‑丝氨酸26.55mg/L～27.15mg/L、丙酮酸钠52mg/L～58mg/L、氯化镁28.61mg/L～28.38mg/L、L‑苏氨酸53.41mg/L～53.49mg/L、维生素H 0.0025mg/L～0.0045mg/L、无水硫酸镁48.55mg/L～49.54mg/L、L‑丙氨酸4.35mg/L～4.55mg/L、乙醇胺500.0mg/L～2000.0mg/L、D‑泛酸钙2.21mg/L～2.26mg/L、氯化钠6950.5mg/L～7120.5mg/L、L‑天门冬酰胺7.4mg/L～8.5mg/L、氯化胆碱8.88mg/L～9.18mg/L、转铁蛋白40～180mg/L、无水磷酸二氢钠54.15mg/L～54.55mg/L、.利托那韦200.0nmol/L～1000.0nmol/L、L‑天门冬氨酸6.25mg/L～6.75mg/L、叶酸2.45mg/L～2.65mg/L、油酸100.0mg/L～1500.0mg/L、磷酸氢二钠70.52mg/L～73.22mg/L、L‑半胱氨酸盐酸盐16.98mg/L～17.65mg/L、白介素410mg/L～80mg/L、i‑肌醇12.2mg/L～12.7mg/L、人血清白蛋白500.0mg/L～3000.0mg/L、庆大霉素1×103～5×106U/L、七水硫酸锌56.431mg/L～210.433mg/L、重组人胰岛素0.5mg/L～5mg/L、L‑谷氨酸7.25mg/L～7.55mg/L、抗坏血酸25.0mg/L～150.0mg/L、烟酰胺1.96mg/L～2.0mg/L、L‑精氨酸盐酸盐145.5mg/L～148.5mg/L、L‑脯氨酸17.15mg/L～17.25mg/L、IL‑77μg/L～9μg/L、IL‑153μg/L～5μg/L、亚油酸100.0mg/L～1200.0mg/L、盐酸吡哆醛1mg/L～2mg/L、孕酮8mg/L～60mg/L、L‑胱氨酸盐酸盐30.19mg/L～32.29mg/L、L‑色氨酸8.72mg/L～9.22mg/L、人过氧化氢酶200.0mg/L～2200.0mg/L、盐酸吡哆醇0.028mg/L～0.035mg/L、蛋氨酸脑啡肽385mg/L～425mg/L、干细胞生长因子5mg/L～40mg/L、L‑酪氨酸38.2mg/L～39.4mg/L、核黄素0.213mg/L～0.219mg/L、甘氨酸18.35mg/L～19.25mg/L、L‑缬氨酸52.45mg/L～53.65mg/L、白介素217～70mg/L、盐酸硫胺2.12mg/L～2.18mg/L、维生素C 0.5～5mg/L、L‑组氨酸盐酸盐31.23mg/L～32.48mg/L、D‑葡萄糖3110mg/L～3165mg/L、胸苷0.312mg/L～0.386mg/L、L‑异亮氨酸54.21mg/L～54.83mg/L、次黄嘌呤1mg/L～3mg/L、维生素B120.61mg/L～0.72mg/L、NaHCO33.0mmol/L～29mmol/L。