[发明专利]一种包含人类X染色体40个InDel遗传多态性位点的复合扩增检测试剂盒

摘要

本发明公开了一种包含人类X染色体40个InDel遗传多态性位点的复合扩增检测试剂盒，通过引物组同时复合扩增40个X染色体InDel位点和1个性别鉴定位点。与传统的STR遗传标记相比，本发明利用的插入缺失遗传标记具有突变率低、灵敏度高的优势；本发明试剂盒涉及的多态性检测位点结合了InDel遗传标记的优点与X染色体特殊的遗传特点，能够为疑难或特殊案件的鉴定提供有效的补充手段。

主权项

1.一种包含人类X染色体40个InDel遗传多态性位点的复合扩增检测试剂盒，其特征在于，通过引物组同时复合扩增40个X染色体InDel位点和1个性别鉴定位点，所述X染色体InDel位点在dbSNP数据库中的编号包括：rs3859989、rs57608175、rs4030406、rs3216913、rs2308280、rs59605609、rs56820003、rs79829945、rs16397、rs61260787、rs2307741、rs25581、rs201342692、rs143123845、rs10699224、rs1160845、rs2308033、rs57843641、rs3077884、rs147741942、rs71671860、rs35574346、rs376744795、rs34763847、rs149102585、rs16367、rs10671504、rs58595330、rs16637、rs36094418、rs17394、rs2307707、rs16368、rs3215490、rs60283667、rs3048996、rs35954471、rs363794、rs45449991、rs199731653；所述性别鉴定位点为Amelogenin；/n所述引物组包括：扩增rs3859989的引物对SEQ ID No.01和SEQ ID No.02；扩增rs57608175的引物对SEQ ID No.03和SEQ ID No.04；扩增rs4030406的引物对SEQ IDNo.05和SEQ ID No.06；扩增rs3216913的引物对SEQ ID No.07和SEQ ID No.08；扩增rs2308280的引物对SEQ ID No.09和SEQ ID No.10；扩增rs59605609的引物对SEQ IDNo.11和SEQ ID No.12；扩增rs56820003的引物对SEQ ID No.13和SEQ ID No.14；扩增rs79829945的引物对SEQ ID No.15和SEQ ID No.16；扩增rs16397的引物对SEQ ID No.17和SEQ ID No.18；扩增rs61260787的引物对SEQ ID No.19和SEQ ID No.20；扩增rs2307741的引物对SEQ ID No.21和SEQ ID No.22；扩增rs25581的引物对SEQ ID No.23和SEQ IDNo.24；扩增rs201342692的引物对SEQ ID No.25和SEQ ID No.26；扩增rs143123845的引物对SEQ ID No.27和SEQ ID No.28；扩增rs10699224的引物对SEQ ID No.29和SEQ IDNo.30；扩增rs1160845的引物对SEQ ID No.31和SEQ ID No.32；扩增rs2308033的引物对SEQ ID No.33和SEQ ID No.34；扩增rs57843641的引物对SEQ ID No.35和SEQ ID No.36；扩增rs3077884的引物对SEQ ID No.37和SEQ ID No.38；扩增rs147741942的引物对SEQ IDNo.39和SEQ ID No.40；扩增rs71671860的引物对SEQ ID No.41和SEQ ID No.42；扩增rs35574346的引物对SEQ ID No.43和SEQ ID No.44；扩增rs376744795的引物对SEQ IDNo.45和SEQ ID No.46；扩增rs34763847的引物对SEQ ID No.47和SEQ ID No.48；扩增rs149102585的引物对SEQ ID No.49和SEQ ID No.50；扩增rs16367的引物对SEQ ID No.51和SEQ ID No.52；扩增rs10671504的引物对SEQ ID No.53和SEQ ID No.54；扩增rs58595330的引物对SEQ ID No.55和SEQ ID No.56；扩增rs16637的引物对SEQ ID No.57和SEQ ID No.58；扩增rs36094418的引物对SEQ ID No.59和SEQ ID No.60；扩增rs17394的引物对SEQ ID No.61和SEQ ID No.62；扩增rs2307707的引物对SEQ ID No.63和SEQ IDNo.64；扩增rs16368的引物对SEQ ID No.65和SEQ ID No.66；扩增rs3215490的引物对SEQID No.67和SEQ ID No.68；扩增rs60283667的引物对SEQ ID No.69和SEQ ID No.70；扩增rs3048996的引物对SEQ ID No.71和SEQ ID No.72；扩增rs35954471的引物对SEQ IDNo.73和SEQ ID No.74；扩增rs363794的引物对SEQ ID No.75和SEQ ID No.76；扩增rs45449991的引物对SEQ ID No.77和SEQ ID No.78；扩增rs199731653的引物对SEQ IDNo.79和SEQ ID No.80；扩增Amelogenin的引物对SEQ ID No.81和SEQ ID No.82；各引物对中至少有一条引物的5’端标记有荧光染料；/n复合扩增引物对分为四组：/n第一组：SEQ ID No.1至SEQ ID No.16、SEQ ID No.81、SEQ ID No.82；/n第二组：SEQ ID No.17至SEQ ID No.36；/n第三组：SEQ ID No.37至SEQ ID No.56；/n第四组：SEQ ID No.57至SEQ ID No.80；/n同一组复合扩增引物对标记相同的荧光染料，各组复合扩增引物对标记的荧光染料互不相同。/n