[发明专利]YN87448病毒毒株全基因组序列及其克隆方法

摘要

一种YN87448病毒毒株全基因组序列及其克隆方法，该克隆方法将全基因分1 5片段，用RT-PCR方法扩增相应cDNA，连入pGEM-T载体，利用通用引物测序，在病毒基因序旬扩增引物设计上，选择甲病毒保守区域的核苷酸序列设计引物，引物3’末端尽可能终止在简并密码子较少的氨基酸位置且最后一个碱基终止在氨基酸密码子的第二位碱基，本发明从分子水平阐明了本病毒与其他流行株的差异，对了解病毒的来源进化过程及分子流行病学有重要意义。也表明：它是预防辛德毕斯脑炎流行的最佳制备疫苗的候选株。

主权项

1、一种YN87448病毒毒株全基因组序列，其特征在于：其全长包括11717个核苷酸，不包括5’端的帽子结构、3’端的多聚核苷酸尾，序列如下：ATTGGCGGCGTAGTACACACTATTGAATTAAACAGCCGACCAATTGCACTACCATCACAATGGAGAAGCCAGTAGTTAACGTAGACGTAGACCCTCAGAGTCCGTTTGTCGTGCAACTGCAAAAGAGCTTCCCGCAATTTGAGGTAGTAGCACAGCAGGTCACTCCAAATGACCATGCTAATGCCAGAGCATTTTCGCATCTGGCCAGTAAACTAATCGAGCTGGAGGTTCCTACCACAGCGACGATTTTGGACATAGGCAGCGCACCGGCTCGTAGAATGTTTTCCGAGCACCAGTACCATTGCGTTTGCCCCATGCGTAGTCCAGAAGACCCGGACCGCATGATGAAATATGCCAGCAAACTGGCGGAAAAAGCATGTAAGATTACAAACAAGAACTTGCATGAGAAGATCAAGGACCTCCGGACCGTACTTGATACACCGGATGCTGAAACGCCATCACTCTGCTTCCACAACGATGTTACCTGCAACACGCGTGCCGAGTACTCCGTCATGCAGGACGTGTACATCAACGCTCCCGGAACTATTTACCACCAGGCTATGAAAGGCGTGCGGACCCTGTACTGGATTGGCTTCGACACCACCCAGTTCATGTTCTCGGCTATGGCAGGTTCGTACCCTGCATACAACACCAACTGGGCCGACGAAAAAGTCCTTGAAGCGCGTAACATCGGACTCTGCAGCACAAAGCTGAGTGAAGGCAGGACAGGAAAGTTGTCGATAATGAGGAAGAAGGAGTTTAAGCCCGGGTCACGGGTTTATTTCTCCGTTGGATCGACACTTTACCCAGAACACAGAGCCAGCTTGCAGAGCTGGCATCTTCCATCGGTGTTCCACTTGAAAGGAAAGCAGTCGTACACTTGCCGCTGTGATACAGTGGTGAGCTGCGAAGGCTACGTAGTGAAGAAAATCACCATCAGTCCCGGGGTCACGGGAGAAACCGTGGGATACGCGGTTACATACAATAGCGAGGGCTTCTTGCTATGCAAAGTTACCGATACAGTAAAAGGAGAACGGGTATCGTTCCCCGTGTGCACGTATATCCCGGCCACCATATGCGATCAGATGACCGGCATAATGGCCACGGATATCTCACCTGACGATGCACAAAAACTTCTGGTTGGGCTCAACCAGCGAATCGTCATTAACGGTAAGACTAACAGGAACACCAATACCATGCAAAATTACCTTCTGCCAATCATTGCACAAGGGTTCAGCAAATGGGCCAAGGAGCGCAAAGCAGATCTTGACAATGAAAAAATGCTGGGCACCAGAGAGCGCAAGCTTACATATGGCTGCTTGTGGGCGTTTCGCACTAAGAAAGTGCACTCGTTCTATCGCCCACCTGGAACGCAGACCAGCGTAAAAGTCCCAGCCTCTTTTAGCGCTTTCCCCATGTCATCCGTATGGACTACCTCTTTGCCCATGTCGCTGAGGCAGAAGGTGAAATTGGCATTACAACCAAAGAAGGAGGAAAAACTGCTGCAAGTCCCGGAGGAATTAGTTATGGAGGCCAAGGCTGCTTTCGAGGATGCTCAGGAGGAATCCAGAGCGGAGAAGCTCCGAGAAGCACTCCCACCATTAGTGGCAGACAAAGGTATCGAGGCAGCTGCGGAAGTTGTCTGCGAAGTGGAGGGGCTCCAGGCGGACATCGGAGCAGCACTCGTCGAAACCCCGCGCGGTCATGTAAGGATAATACCTCAAGCAAATGACCGTATGATCGGACAGTATATCGTTGTCTCGCCGATCTCTGTGCTGAAGAACGCTAAACTCGCACCAGCACACCCGCTGGCAGACCAGGTTAAGATCATAACGCACTCCGGAAGATCAGGAAGGTATGCAGTCGAACCATACGACGCTAAAGTACTGATGCCAGCAGGAAGTGCTGTACCATGGCCAGAATTCTTAGCACTGAGTGAGAGCGCCACGCTAGTGTACAACGAAAGAGAGTTTGTGAACCGCAAGCTGTACCATATTGCCATGCACGGTCCCGCTAAGAATACAGAAGAGGAGCAGTACAAGGTTACAAAGGCAGAGCTCGCAGAAACAGAGTACGTGTTTGACGTGGACAAGAAGCGATGCGTTAAGAAGGAAGAAGCCTCAGGACTTGTCCTTTCGGGAGAACTGACCAACCCGCCCTATCACGAACTAGCTCTTGAGGGACTGAAGACTCGACCCGCGGTCCCGTACAAGGTTGAAACAATAGGAGTGATAGGCACACCAGGATCGGGCAAGTCAGCTATCATCAAGTCAACTGTCACGGCACGTGATCTTGTTACCAGCGGAAAGAAAGAAAACTGCCGCGAAATTGAGGCCGACGTGCTACGGCTGAGGGGCATGCAGATCACGGCGAAGACAGTGGATTCGGTTATGCTCAACGGATGCCACAAAGCCGTAGAAGTGCTGTATGTTGACGAAGCGTTCGCGTGCCACGCAGGAGCACTACTTGCCTTGATTGCAATCGTCAGACCCCGTAAGAAGGTAGTACTATGCGGAGACCCTAAGCAATGCGGATTCTTCAACATGATGCAACTAAAGGTACATTTCAACCACCCTGAAAAAGACATATGTACCAAGACATTCTACAAGTTTATCTCCCGACGTTGCACACAGCCAGTCACGGCTATTGTATCGACACTGCATTACGATGGAAAAATGAAAACCACAAACCCGTGCAAGAAGAACATCGAAATCGACATTACAGGGGCCACGAAGCCGAAGCCAGGGGACATCATCCTGACATGTTTCCGCGGGTGGGTTAAGCAACTGCAAATCGACTATCCCGGACATGAGGTAATGACAGCCGCGGCCTCACAAGGGCTAACCAGAAAAGGAGTATATGCCGTCCGGCAAAAAGTCAATGAAAACCCGCTGTACGCGATCACATCAGAGCATGTGAACGTGTTGCTCACCCGCACTGAGGACAGGCTAGTATGGAAAACTTTACAGGGCGACCCATGGATTAAGCAGCTCACTAACGTACCTAAAGGAAATTTTCAGGCCACCATCGAGGACTGGGAAGCTGAACACAAGGGAATAATTGCTGCGATAAACAGTCCCGCTCCCCGTACCAATCCGTTCAGCTGCAAGACTAACGTTTGCTGGGCGAAAGCACTGGAACCGATACTGGCCACGGCCGGTATCGTACTTACCGGTTGCCAGTGGAGCGAGCTGTTCCCACAGTTTGCGGATGATAAACCACACTCGGCCATCTACGCCTTAGACGTAATTTGCATTAAGTTTTTCGGCATGGACTCGACAAGCGGACTGTTTTCCAAACAGAGCATCCCGTTAACGTACCATCCTGCCGACTCAGCGAGGCCAGTAGCTCATTGGGACAACAGCCCAGGAACACGCAAGTATGGGTACGATCACGCCGTTGCCGCCGAACTCTCCCGTAGATTTCCGGTGTTCCAGCTAGCTGGGAAAGGCACACAGCTTGATTTGCAGACGGGCAGAACTAGAGTTATCTCTGCACAGCATAACTTGGTCCCAGTGAACCGCAATCTCCCTCACGCCTTAGTCCCCGAGCACAAGGAGAAACAACCCGGCCCGGTCGAAAAATTCTTGAGCCAGTTCAAACACCACTCCGTACTTGTGATCTCAGAGGAAAAAATTGAAGCTCCCCACAAGAGAATCGAATGGATCGCCCCGATTGGCATAGCCGGCGCAGATAAGAACTACAACCTGGCTTTCGGGTTTCCGCCGCAGGCACGGTACGACCTGGTGTTCATCAATATTGGAACTAAATACAGAAACCATCACTTTCAACAGTGCGAAGACCACGCGGCGACCTTGAAAACCCTTTCGCGTTCGGCCCTGAACTGCCTTAACCCCGGAGGCACCCTCGTGGTGAAGTCCTACGGTTACGCCGACCGCAATAGTGAGGACGTAGTCACCGCTCTTGCCAGAAAATTTGTCAGAGTGTCTGCAGCGAGGCCAGAGTGCGTCTCAAGCAATACAGAAATGTACCTGATTTTCCGACAACTAGACAACAGCCGCACACGACAATTCACCCCGCATCATTTGAATTGTGTGATTTCGTCCGTGTACGAGGGTACAAGAGACGGAGTTGGAGCCGCACCGTCGTACCGTACTAAAAGGGAGAACATTGCTGATTGTCAAGAGGAAGCAGTTGTCAATGCAGCCAATCCACTGGGCAGACCAGGAGAAGGAGTCTGCCGTGCCATCTATAAACGTGGGCCGAACAGTTTCACCGATTCAGCCACAGAGACAGGCACCGCAAAACTGACTGTGTGCCAAGGAAAGAAAGTGATCCACGTGGTTGGCCCTGATTTCCGGAAACACCCAGAGGCAGAAGCCCTGAAATTGCTGCAAAACGCCTACCATGCAGTGGCAGACTTAGTAAATGAACATAATATCAAGTCTGTCGCCATCCCATTGCTATCTACAGGCATTTACGCAGCCGGAAAAGACCGCCTTGAGGTATCACTTAACTGCTTGACAACCGCGCTAGACAGAACTGATGCGGACGTAACCATCTACTGCCTGGATAAGAAGTGGAAGGAAAGAATCGACGCGGTGCTCCAACTTAAGGAGTCTGTAACTGTGCTGAAGGATGAGGATATGGAGATCGACGACGAGTTAGTATGGATCCATCCGGACAGTTGCCTGAAGGGAAGACAGGGATTCAGTACTACAAAAGGAAAGTTGTATTCGTACTTTGAAGGCACCAAATTCCATCAAGCAGCAAAAGATATGGCGGAGATAAAGGTCCTGTTCCCAAATGACCAGGAAAGCAACGAACAACTGTGTGCCTACATATTGGGGGAGACCATGGAAGCAATCCGCGAAAAATGCCCGGTCGACCACAACCCGTCGTCTAGCCCGCCAAAAACGCTGCCGTGCCTCTGTATGTATGCCATGACGCCAGAAAGGGTCCACAGACTCAGAAGCAATAACGTTAAAGAAGTTACAGTATGCTCCTCCACCCCCCTTCCAAAGTACAAAATCAAGAATGTTCAGAAGGTTCAGTGCACAAAAGTAGTCCTGTTTAACCCGCATACCCCCGCATTCGTTCCCGCCCGTAAGTACATAGAAGCACCAGAACAGCCTGCAGCTCCGCCTGCACAGGCCGAGGAGGCCCCCGGAGTTGTAGCGACACCAACACCACCTGCAGCTGATAACACCTCGCTTGATGTCACGGACATCTCACTGGACATGGAAGACAGTAGCGAAGGCTCACTCTTTTCGAGCTTTAGCGGATCGGACAACTCTATTACTAGTATGGACAGTTGGTCGTCAGGACCTAGTTCACTAGAGATAGTAGACCGAAGGCAGGTGGTGGTGGCTGACGTCCATGCCGTCCAAGAGCCTGCCCCTGTTCCACCGCCAAGGCTAAAGAAGATGGCCCGCCTGGCAGCGGCAAGAATGCAGGAAGGGCCAACTCCACCGGCAAGCACCAGCTCTGCGGACGAGTCCCTTCACCTTTCTTTTGATGGGGTATCTATGTCCTTCGGATCCCTTTTCGACGGAGAGATGGTCCGCTTGGCAGCGGCACAACCCCCGGCAACATGCCCTACGGATGTGCCTATGTCTTTCGGATCGTTTTCCGACGGAGAGATTGAGGAGTTGATCCGCAGAGTAACCGAGTCGGAGCCCGTCCTGTTTGGGTCATTTGAACCGGGCGAAGTGAACTCAATTATATCGTCCCGTTCAGCCGTATCTTTTCCACCACGCAAGCAGAGACGTAGACGCAGGAGCAGGAGGACCGAATACTGACTAACCGGGGTAGGTGGGTACATATTTTCGACGGACACAGGCCCTGGGCACTTGCAAAAGAAGTCCGTTCTGCAGAACCAGCTTACAGAACCGACCTTGGAGCGCAATGTTCTGGAAAGAATCTACGCCCCGGTGCTCGACACGTCGAAAGAGGAACAGCTCAAACTCAGGTACCAGATGATGCCCACCGAAGCCAACAAAAGCAGGTACCAGTCTCGAAAAGTAGAAAACCAGAAAGCCATAACCACTGAGCGACTGCTTTCAGGGCTACGACTGTATAACTCTGCCACAGATCAGCCAGAATGCTATAAGATCACCTACCCGAAACCATCGTATTCCAGCAGTGTACCAGCTAACTACTCTGACCCAAAGTTTGCTGTAGCTGTTTGTAACAACTATCTGCATGAGAATTACCCGACGGTAGCATCTTATCAGATCACCGACGAGTACGATGCTTACTTGGATATGGTAGACGGGACAGTCGCTTGCCTAGATACTGCAATTTTTTGCCCCGCCAAGCTTAGAAGTTACCCGAAAAGACACGAGTATAGAGCCCCAAACATCCGCAGTGCGGTTCCATCAGCGATGCAGAACACGTTGCAAAACGTGCTCATTGCCGCGACTAAAAGAAACTGCAACGTCACACAAATGCGTGAACTGCCAACACTGGACTCAGCGACATTCAACGTTGAATGCTTTCGAAAATATGCATGCAATGACGAGTATTGGGAGGAGTTTGCCCGAAAGCCAATTAGGATCACTACTGAGTTCGTTACCGCATACGTGGCCAGACTGAAAGGCCCTAAGGCCGCCGCACTGTTCGCAAAGACGCATAATTTGGTCCCATTGCAAGAAGTGCCTATGGATAGATTCGTCATGGACATGAAAAGAGACGTGAAAGTTACACCTGGCACGAAACACACAGAAGAAAGACCGAAAGTACAAGTGATACAAGCCGCAGAACCCCTGGCGACCGCTTACCTATGCGGGATCCACCGGGAGTTAGTGCGCAGGCTTACAGCCGTTTTGCTACCCAACATTCACACGCTCTTTGACATGTCGGCGGAGGACTTTGATGCAATCATAGCAGAACACTTCAAGCAAGGTGACCCGGTACTGGAGACGGATATCGCCTCGTTCGACAAAAGCCAAGACGACGCTATGGCGTTAACCGGCCTGATGATCTTGGAAGACCTGGGTGTGGACCAACCACTACTCGACTTGATCGAGTGCGCCTTTGGAGAAATATCATCCACCCATCTGCCCACGGGTACCCGTTTCAAATTCGGGGCGATGATGAAATCCGGAATGTTCCTCACGCTCTTTGTCAACACAGTTCTGAATGTCGTTATCGCCAGCAGAGTATTGGAGGAGCGGCTTAAAACGTCCAAATGTGCAGCATTTATCGGCGACGATAACATTATACACGGAGTAGTATCTGACAAAGAAATGGCTGAGAGGTGTGCCACCTGGCTCAACCTGGAGGTTAAGATCATTGACGCAGTCATCGGCGAGAGACCACCTTACTTCTGCGGTGGATTCATCTTGCAAGATTCGGTTACCTCCACAGCGTGTCGCGTGGCGGACCCCTTGAAAAGGCTGTTTAAGTTGGGTAAACCGCTCCCAGCCGACGACGAGCAAGACGAAGACAGAAGACGCGCTCTGCTAGATGAAACAAAGGCGTGGTTTAGAGTAGGTATAACAGACACCTTAGCAGTGGCCGTGGCAACTCGGTATGAGGTAGACAACATCACACCTGTCCTGCTGGCATTGAGAACTTTTGCCCAGAGCAAAAGAGCATTTCAAGCCATCAGAGGGGAAATAAGGCATCTCTACGGTGGTCCTAAATAGTCAGCATAGTACATTTCATCTGACTAATACCACAACACCACCACCATGAATAGAGGATTCTTTAACATGCTCGGCCGCCGCCCCTTCCCAGCCCCCACTGCCATGTGGAGGCCGCGGAGAAGGAGGCAGGCGGCCCCGATGCCTGCCCGCAATGGGCTGGCTTCCCAAATCCAGCAACTGACCACAGCCGTCAGTGCCCTAGTCATTGGACAGGCAACTAGACCTCAAACCCCACGCCCACGCCCGCCGCCGCGCCAGAAGAAGCAGGCGCCAAAGCAACCACCGAAGCGGAAGAAACCAAAAACACAGGAGAAGAAGAAGAAGCAACCTGCAAAACCCAAACCCGGAAAGAGACAGCGTATGGCACTTAAGTTGGAGGCCGACAGACTGTTCGACGTAAAAAATGAGGACGGAGATGTCATCGGGCACGCACTGGCCATGGAAGGAAAGGTAATGAAACCTCTCCACGTGAAAGGAACTATTGACCACCCTGTGCTATCAAAGCTCAAATTCACCAAGTCGTCAGCATACGACATGGAGTTCGCACAGTTGCCGGTCAACATGAGAAGTGAGGCGTTCACCTACACCAGTGAACACCCTGAAGGGTTCTACAACTGGCACCACGGAGCGGTGCAGTATAGTGGAGGCAGATTTACCATCCCCCGCGGAGTAGGAGGCAGAGGAGACAGTGGTCGTCCGATTATGGATAACTCAGGCCGGGTTGTCGCGATAGTCCTCGGAGGGGCTGATGAGGGAACAAGAACCGCCCTTTCGGTCGTCACCTGGAATAGCAAAGGGAAGACAATCAAGACAACCCCGGAAGGGACAGAAGAGTGGTCTGCTGCACCACTGGTCACGGCCATGTGCTTGCCTGGAAACGTGAGCTTCCCATGCAATCGCCCGCCCACATGCTACACCCGCGAACCATCCAGAGCTCTCGACATCCTCGAAGAGAACGTGAACCACGAGGCCTACGACACCCTGCTCAACGCCATATTGCGGTGCGGATCGTCCGGCAGAAGTAAAAAAAAAAGCGTCACTGACGACTTTACCTTGACCAGCCCGTACTTGGGCACATGCTCGTACTGTCACCATACTGAACCGTGCTTTAGCCTGATTAAGATCGAGCAGGTCTGGGATGAAGCGGACGACAACACCATACGCATACAGACTTCCGCCCAGTTTGGATACGACCAAAGCGGAGCAGCAAGCTCAAATAAGTACCGCTACATGACGCTCGAGCAGGATCATACTGTCAAAGAAGGCACCATGGATGACATCAAGATCAGCACCTCAGGACCGTGTGGAAGGCTTAGCTACAAAGGATACTTTCTCCTCGCGAAGTGTCCTCCAGGGGACAGCGTAACGGTTAGCATAGCGAGTAGCAACTCAGCAACGTCATGCACAATGGCCCGCAAGATAAAACCAAAATTCGTGGGACGGGAAAAATATGACCTACCTCCCGTTCACGGTAAGAAGATTCCTTGCACAGTGTACGACCGTCTGAAAGAAACAACCGCCGGCTACATCACTATGCACAGGCCGGGACCGCACGCCTATACATCCTATCTGAAGGAATCATCAGGGAAAGTTTACGCGAAGCCACCATCCGGGAAGAACATTACGTACGAGTGCAAGTGCGGCGATTACAAGACCGGAACCGTTACGACCCGTACCGAAATCACGGGCTGCACCGCCATCAAGCAGTGCGTCGCCTATAAGAGCGACCAAACGAAGTGGGTCTTCAACTCGCCGGACTCTATCAGACACGCCGACCACACGGCCCAAGGGAAATTGCATTTGCCTTTCAAGCTGATCCCGAGTACCTGCATGGTCCCTGTTGCCCACGCGCCGAACGTAGTACACGGCTTTAAACACATCAGCCTCCAATTAGACACAGACCATCTGACATTGCTCACCACCAGGAGACTAGGGGCAAACCCGGAACCAACCACTGAATGGATCATCGGAAAGACGGTTAGAAACTTCACCGTCGACCGAGATGGCCTGGAATACATATGGGGCAATCACGAACCAGTAAGGGTCTATGCCCAAGAGTCTGCACCAGGAGACCCTCACGGATGGCCACACGAAATAGTACAGCATTACTATCATCGCCATCCTGTGTACACCATCTTAGCCGTCGCATCAGCTGCTGTGGCGATGATGATTGGCGTAACTGTTGCAGCATTATGTGCCTGTAAAGCGCGCCGTGAGTGCCTGACGCCATATGCCCTGGCCCCAAATGCCGTGATTCCAACTTCGCTGGCACTTTTGTGCTGTGTTAGGTCGGCTAATGCTGAAACATTCACCGAGACCATGAGTTACTTATGGTCGAACAGCCAGCCGTTCTTCTGGGTCCAGCTGTGTATACCTCTGGCCGCTGTCATCGTTCTAATGCGCTGTTGCTCATGCTGCCTGCCTTTTTTAGTGGTTGCCGGCGCCTACCTGGCGAAGGTAGACGCCTACGAACATGCGACCACTGTTCCAAATGTGCCACAGATACCATATAAGGCACTTGTTGAAAGGGCAGGGTACGCCCCGCTCAGTTTGGAGATTACTGTCATGTCCTCGTAGGTTTTGCCTTCCACCAACCAAGAGTACATCACCTGCAAATTCACCACTGTGGTCCCCTCCCCTAAAGTCAGATGCTGCGGCTCCTTGGAATGTCAGCCCGCGGCTCACGCAGATTATACCTGCAAGGTCTTTGGAGGGGTGTACCCCTTCATGTGGGGAGGAGCACAATGTTTTTGCGACAGTGAGAACAGCCAGATGAGTGAGGCGTACGTCGAATTGTCAGCAGATTGCGCGACTGACCACGCGCAGGCGATTAAGGTGCATACTGCCGCGATGAAAGTAGGACTGCGTATAGTGTACGGGAACACTATCAGTTTCCTAGATGTGTACGTGAACGGAGTCACACCAGGAACGTCTAAAGACCTGAAAGTCATAGCTGGACCAATTTCAGCATTGTTTACACCATTCGATCACAAGGTCGTTATCCATCGCGGCCTGGTGTACAACTATGACTTTCCGGAATACGGAGCGATGAAACCAGGAGCGTTTGGAGACATTCAAGCTACCTCCTTGACTAGCAAAGACCTCATCGCCAGCACAGATATTAGGCTACTCAAGCCTTCCGCCAAGAACGTGCATGTCCCGTACACGCAGGCCGCATCTGGATTCGAGATGTGGAAAAACAACTCAGGCCGCCCACTGCAGGAAACCGCCCCTTTTGGGCGCAAGATTGCAGTCAATCCGCTTCAGGCGGTGGACTGCTCATACGGGAACATTCCCATCTCTATTGACATCCCGAACGCTGCCTTTATCAGGACATCAGATGCACCACTGGTCTCAACAGTCAAATGTGATGTCAGTGAGTGCACTTATTCAGCGGACTTCGGCGGGATGGCTACCCTGCAGTATGTATCCGACCGCGAAGGACAATGCCCTGTACATTCGCATTCGAGCACAGCAACCCTCCAAGAGTCGACAGTTCATGTCCTGGAGAAAGGAGCGGTGACAGTACACTTCAGCACCGCGAGCCCACAGGCAAACTTCATTGTATCGCTGTGTGGTAAGAAGACAACATGCAATGCAGAATGCAAACCACCAGCTGATCATATCGTGAGCACCCCGCACAAAAATGACCAAGAATTCCAAGCCGCCATCTCAAAAACTTCATGGAGTTGGCTGTTTGCCCTTTTCGGCGGCGCCTCGTCGCTATTAATTATAGGACTTATGATTTTTGCTTGCAGCATGATGCTGACTAGCACACGAAGATGACCGCTACGCCCCAATGACCCGACCAGCAAAACTCGATGTACTTCCGAGGAACTGATGTGCATAATGCATCAGGCTGGTATATTAGATCCCCGCTTACCGCGGGCAATATAGCAACACCAAAACTCGACGTATTTCCGAGGAAGCGCAGTGCATAATGCTGCGCAGTGTTGCCAAATAATCACTATATTAACCATTTATTCAGCGGACGCCAAAACTCAATGTATTTTTGAGGAAGCATGGTGCATAATGCCATGCAGCGTCTGCATAACTTTTTATTATTTCTTTTATTAATCAACAAAATTTTGTTTTTAACATTTC。