[发明专利]人类17号染色体1区3带3亚带区域内人肿瘤相关基因无效
| 申请号： | 99113909.7 | 申请日： | 1999-07-23 |
| 公开（公告）号： | CN1281899A | 公开（公告）日： | 2001-01-31 |
| 发明（设计）人： | 万大方;赵新泰;顾健人 | 申请（专利权）人： | 上海市肿瘤研究所 |
| 主分类号： | C12N15/12 | 分类号： | C12N15/12;C12N15/63;A61K48/00 |
| 代理公司： | 上海华东专利事务所 | 代理人： | 费开逵 |
| 地址： | 200032 *** | 国省代码： | 上海;31 |
| 权利要求书： | 查看更多 | 说明书： | 查看更多 |
| 摘要： | 本发明涉及到人17号染色体短臂13.3位点的癌相关基因(cDNA)的分离和克隆。用对应于17p13.3区段内926位点的噬菌体人工染色体(PAC)579号(P579)为探针筛选人正常肝cDNA文库得到3个新基因HC56、HC71、HC90并得到全长核苷酸序列和编码的氨基酸。通过Northern、Southern杂交,证明它们与肝癌相关,体外实验证明它们可以抑制肝癌细胞7721的生长。因此,3个基因有望作为药物或试剂应用于肿瘤的诊断、治疗和预防。 | ||
| 搜索关键词： | 人类 17 染色体 区域 内人 肿瘤 相关 基因 | ||
【主权项】：
1．一种人类17号染色体1区3带3亚带区域内人肿瘤相关基因，其特征在于人肿瘤相关基因HC56具有下列全长核苷酸序列及其所编码的氨基酸序列：1CTAGAATTCAGCGGCCGCTGAATTCAGAATGGCCGTGTGATTGCTC4647TGTGCCCATTACCCTGTGGCCGTGTGACTGCTGCTGTGCCCATTACCC9495TGTGGCCGTGTGATTGCTGCTGTGCCCATTACCCTGTGGCCGTGTGAT142143TGCTCTGTGCCCATTATCCTGTGGCCGTGTGATTGCTCTGTGCCCATT190191ACCCTGTGGCCGTGTGATTGCTCTGTGCCCATTACCCTGTGGCCGTGT238239GATTGCTCTGTGCCCATTATCCTGTGGCCGTGTGATTGCTCTGTGCCC286287ATTATCCTGTGGCCGTGTGATTGCTCTGTGCCCATTATCCTGTGGCCG334335TGTGATTGCTCTGTGCCCATTATCCTGTGGCCGTGTGATTGCTGCTGT382383GCCCATTACCCTGTGGCCGTGTGATTGCTCTGTGCCCATTATCCTGTG430431GCCGTGTGATTGCTCTGTGCCCATTATCCTGTGGCTGTGTGATTGCTC478479TGTGCCCATTATCCTGTAGCCGTGTGATTGCTTCTGTGCCCATTACCC526527TGTGGCTGTGTGATTGCTGCTGTGCCCATTATCCTGTGGCTGTGTGAT574575TGCTGCTGTGCCCATTACCCTGTGGCTGTGTGATGCTCTGTGCCCATT622623ACCCTGTGGCTGTGTGATTGCTCTGTCCCATTATCCTGTGGCCGTGTG670671ATTGCTGCTGTGCCTGTTACCCTGTGGCTGTGTGATTGCTCTGTGCCC718719ATTACCCTGTGGCTATGCTCCCTTCATCTGTCATGAGAAGCTCAGCTG766767TCATGTCCTGTGGTACATGCTCAGTGGCCCCTGTAGTTTGTACTGTCC814815TCCTATTTCTAAACCCCTCTCCCCACATCCTCTGCTGGCCCAGCCTTT862863GCTGGAGGGTCTCGCCTCAGCAGCCCAGCTTTTTCTTTTCACACACTT910911TTCCTGAAGGATCTCATTCACCGTCTTGGTTCCGGTGATCCCCTCTGG958959GCAGATACCTCTCAGAATCACATTTCCCAGCTTACTTCCCTCCTGAAC10061007TTCCACCTGGCATTTCCGTTGCTGGAGGACATCTGTACCTTGATGGCC1054续后页1055AAAGCTGAACTCATCTTTCCTCACACCTGCTCTGATTCTCCTCCATTC11021103CCTGTATGTGATGTCACCTGGTGGCCTCCCAGTTCCCAGGCTGGAGAG11501151CTCGGAAGCCATTCTGGATTCCTCGGCCAAGTCCTTCTGACTCCAGCT11981199GTGCAGTGGCTCTTGTAGTCATCCATTCTCCCCGTCCGCTGATGTCCT12461247TTAAAACCCTTGTCATCTCAAACCATGACAGCCTACTAACAGCAGTGG12941295CCATCTGGAAACATTTTCACTATACTGTCTTATTTGGCTTGTCTGTGT13421343GCAGGACCCTTGAACATCTGTGAAGAAATGACTATTCTGCATGGAGGC13901MetThrI1eLeuHisGlyGly71391TTCTTGCTGGCCGAGCAGCTGTTCCACCCTAAGGCACTGGCAGAATTA14388PheLeuLeuAlaGluGlnLeuPheHisProLysAlaLeuAlaGluLeu231439ACAAAGTCTGACTGGGAACGTGTTGGACGGCCCATCGTGGAGGCCTTA148624ThrLysSerAspTrpGluArgValGlyArgProIleValGluAlaLeu391487AGGGAGATCTCCTCGGCTGCAGCACACTCCCAGCCCTTTGCCTGGAAG153440ArgGluIleSerSerAlaAlaAlaHisSerGlnProPheAlaTrpLys551535AAGAAAGCCCTGATCATCATCTGGGCCAAGGTTCTGCAGCCGCACCCC158256LysLysAlaLeuIleIleIleTrpAlaLysValLeuGlnProHisPro711583GTGACCCCGTCCGACACAGAGACACGGTGGCAGGAAGACCTGTTCTTC163072ValThrProSerAspThrGluThrArgTrpGlnGluAspLeuPhePhe871631TCGGTGGGCAACATGATCCCCACCATCAACCACACCATCCTCTTCGAG167888SerValGlyAsnMetIleProThrIleAsnHisThrIleLeuPheGlu1031679CTGCTCAAATCCCTGGAAGCTTCTGGACTCTTTATCCAGCTCCTGATG1726104LeuLeuLysSerLeuGluAlaSerGlyLeuPheIleGlnLeuLeuMet119续后页1727GCCCTGCCCACCACCATCTGCCATGCAGAACTAGAGCGCTTTCTGGAA1774120AlaLeuProThrThrIleCysHisAlaGluLeuGluArgPheLeuGlu1351775CATGTGACCGTTGACACTTCTGCCGAAGACGTGGCCTTCTTCCTGGAC1822136HisValThrValAspThrSerAlaGluAspValAlaPhePheLeuAsp1511823GTCTGGTGGGAGGTGATGAAGCACAAGGGTCACCCGCAGGACCCCCTG1870152ValTrpTrpGluValMetLysHisLysGlyHisProGlnAspProLeu1671871CTCTCCCAGTTTAGTGCAATGGCCCATAAGTACCTGCCTGCCTTAGAT1918168LeuSerGlnPheSerAlaMetAlaHisLysTyrLeuProAlaLeuAsp1831919GAGTTCCCCCATCCTCCAAAGAGGCTTAGGTCAGACCCAGACGCGTGC1966184GluPheProHisProProLysArgLeuArgSerAspProAspAlaCys1991967CCCACCATGCCCCTGTTGGCCATGCTGCTCCGCGGGCTGACACAGATC2014200ProThrMetProLeuLeuAlaMetLeuLeuArgGlyLeuThrGlnIle2152015CAGAGTCGGATCCTGGGCCCGGGGAGGAAGTGCTGTGCGCTGGCCAAC2062216GlnSerArgIleLeuGlyProGlyArgLysCysCysAlaLeuAlaAsn2312063CTGGCTGACATGCTGACTGTGTTTGCGCTGACAGAGGACGACCCCCAG2110232LeuAlaAspMetLeuThrValPheAlaLeuThrGluAspAspProGln2472111GAGGTGTCTGCAACCGTGTATCTGGACAAACTGGCCACGGTGATCTCT2158248GluValSerAlaThrValTyrLeuAspLysLeuAlaThrValIleSer2632159GTGTGGAACTCGGACACCCAGAATCCCTACCACCAGCAGGCGCTGGCA2206264ValTrpAsnSerAspThrGlnAsnProTyrHisGlnGlnAlaLeuAla279续后页2207GAGAAGGTGAAGGAGGCAGAACGGGATGTCAGCCTGACCTCGCTGGCC2254280GluLysValLysGluAlaGluArgAspValSerLeuThrSerLeuAla2952255AAACTCCCCAGTGAGACCATTTTCGTGGGCTGCGAGTTCCTGCACCAC2302296LysLeuProSerGluThrIlePheValGlyCysGluPheLeuHisHis3112303CTGCTGCGGGAGTGGGGGGAGGAGTTGCAGGCCGTGCTCCGCAGCAGC2350312LeuLeuArgGluTrpGlyGluGluLeuGlnAlaValLeuArgSerSer3272351CAGGGGACAAGTTACGACAGCTACCGGCTGTGCGACAGTCTGACTTCC2398328GlnGlyThrSerTyrAspSerTyrArgLeuCysAspSerLeuThrSer3432399TTCAGCCAGAACGCGACGCTCTACCTGAACCGCACCAGCCTGTCCAAG2446344PheSerGlnAShAlaThrLeuTyrLeuAsnArgThrSerLeuSerLys3592447GAGGACAGGCAGGTGGTCTCTGAGCTGGCGGAGTGTGTCAGGGACTTC2494360GluAspArgGlnValValSerGluLeuAlaGluCysValArgAspPhe3752495CTGAGGAAAACGAGCACGGTGCTGAAGAACAGGGCCTTGGAGGATATC2542376LeuArgLysThrSerThrValLeuLysAsnArgAlaLeuGluAspIle3912543ACAGCTTCCATTGCCATGGCCGTCATCCAGCAGAAGATGGACCGCCAT2590392ThrAlaSerIleAlaMetAlaValIleGlnGlnLysMetAspArgHis4072591ATGGAAGTGTGCTACATTTTTGCCTCTGAGAAGAAGTGGGCCTTCTCG2638408MetGluValCysTyrIlePheAlaSerGluLysLysTrpAlaPheSer4232639GACGAGTGGGTAGCCTGCCTGGGGAGTAACAGGGCCCTCTTCCGAGAG2686424AspGluTrpValAlaCysLeuGlySerAsnArgAlaLeuPheArgGlu439续后页2687CCAGACTTGGTGTTGAGGCTGCTGGAAACAGTGATAGACGTCAGCACA2734440ProAspLeuValLeuArgLeuLeuGluThrValIleAspValSerThr4552735GCTGACAGAGCCATCCCTGAGTCTCAGATCCGGCAGGTGATCCACCTG2782456AlaAspArgAlaIleProGluSerGlnIleArgGlnValIleHisLeu47l2783ATCCTGGAATGTTACGCAGACCTCTCCCTGCCAGGTAAAAATAAAGTC2830472IleLeuGluCysTyrAlaAspLeuSerLeuProGlyLysAsnLysVal4872831CTTGCAGGTATCCTGCGTTCCTGGGGGCGAAAGGGCCTCTCTGAAAAG2878488LeuAlaGlyIleLeuArgSerTrpGlyArgLysGlyLeuSerGluLys5032879TTGCTGGCTTATGTGGAGGGTTTTCAGGAAGACCTCAATACAACTTTT2926504LeuLeuAlaTyrValGluGlyPheGlnGluAspLeuAsnThrThrPhe5192927AACCAGCTCACTCAGAGTGCCTCCGAACAGGGCTTGGCAAAAGCTGTG2974520AsnGlnLeuThrGlnSerAlaSerGluGlnGlyLeuAlaLysAlaVal5352975GCCTCCGTGGCCCGCCTGGTCATAGTGCACCCGGAAGTCACGGTGAAG3022536AlaSerValAlaArgLeuValIleValHisProGluValThrValLys5513023AAAATGTGCAGCCTGGCTGTGGTCAATCTCGGCACCCACAAGTTCCTG3070552LysMetCysSerLeuAlaValValASnLeuGlyThrHisLysPheLeu5673071GCCCAGATTCTCACTGCCTTCCCTGCCCTTAGGTTTGTGGAAGTGCAG3118568AlaGlnIleLeuThrAlaPheProAlaLeuArgPheValGluValGln5833119GGTCCCAATTCATCTGCCACTTTCATGGTGTCATGCCTCAAAGAAACC3166584GlyProAsnSerSerAlaThrPheMetValSerCysLeuLysGluThr599续后页3167GTCTGGATGAAGTTCTCTACACCCAAGGAAGAAAAGCAATTTTTAGAG3214600ValTrpMetLysPheSerThrProLysGluGluLysGlnPheLeuGlu6153215CTCCTGAACTGCCTGATGAGTCCCGTGAAACCCCAAGGGATTCCAGTG3262616LeuLeuAsnCysLeuMetSerProValLysProGlnGlyIleProVal6313263GCTGCTCTTCTTGAGCCAGACGAGGTGCTGAAGGAATTTGTCCTGCCT3310632AlaAlaLeuLeuGluProAspGluValLeuLysGluPheValLeuPro6473311TTCTTGAGGTTAGATGTTGAAGAGGTAGACCTCAGTCTGAGGATCTTC3358648PheLeuArgLeuAspValGluGluValAspLeuSerLeuArgIlePhe6633359ATCCAGACTCTAGAGGCAAACGCGTGTCGAGAGGAATACTGGCTCCAG3406664IleGlnThrLeuGluAlaAsnAlaCysArgGluGluTyrTrpLeuGln6793407ACCTGCTCCCCGTTTCCACTCCTCTTCAGCTTGTGCCAGCTCTTGGAC3454680ThrCysSerProPheProLeuLeuPheSerLeuCysGlnLeuLeuAsp6953455CGCTTCAGCAAATACTGGCAGCTTCCCAAGGAGAAGCGGTGCCTCTCT3502696ArgPheSerLysTyrTrpGlnLeuProLysGluLysArgCysLeuSer7113503TTGGATAGGAAGGATCTAGCGATCCATATCCTGGAGCTCCTGTGTGAG3550712LeuAspArgLysAspLeuAlaIleHisIleLeuGluLeuLeuCysGlu7273551ATTGTATCAGCCAATGCTGAGACCTTCTCCCCGGATGTCTGGATCAAG3598728IleValSerAlaAsnAlaGluThrPheSerProAspValTrpIleLys7433599TCCCTGTCCTGGCTCCACCGCAAGTTAGAACAGCTAGACTGGACTGTG3646744SerLeuSerTrpLeuHisArgLysLeuGluGlnLeuAspTrpThrVal759续后页3647GGCCTGAGGCTGAAGAGCTTCTTCGAGGGGCACTTCAAGTGTGAAGTG3694760GlyLeuArgLeuLysSerPhePheGluGlyHisPheLysCysGluVal7753695CCAGCCACACTTTTTGAGATCTGTAAGCTTTCAGAAGACGAGTGGACC3742776ProAlaThrLeuPheGluIleCysLysLeuSerGluAspGluTrpThr7913743TCCCAGGCCCACCCAGGGTACGGGGCTGGCACGGGGCTCCTGGCCTGG3790792SerGlnAlaHisProGlyTyrGlyAlaGlyThrGlyLeuLeuAlaTrp8073791ATGGAGTGCTGCTGCGTCTCCAGCGGCATCTCGGAGAGGATGCTGTCT3838808MetGluCysCysCysValSerSerGlyIleSerGluArgMetLeuSer8233839CTCTTGGTGGTGGACGTGGGCAATCCTGAGGAGGTCAGACTGTTCAGC3886824LeuLeuValValAspValGlyAsnProGluGluValArgLeuPheSer8393887AAAGGCTTTCTGGTGGCCCTGGTGCAAGTCATGCCTTGGTGCAGCCCT3934840LysGlyPheLeuValAlaLeuValGlnValMetProTrpCysSerPro8553887AAAGGCTTTCTGGTGGCCCTGGTGCAAGTCATGCCTTGGTGCAGCCCT3934840LysGlyPheLeuValAlaLeuValGlnValMetProTrpCysSerPro8553935CAGGAGTGGCAGCGCCTTCACCAGCTGACCAGGAGACTGCTGGAGAAG3982856GlnGluTrpGlnArgLeuHisGlnLeuThrArgArgLeuLeuGluLys8713983CAGCTCCTCCATGTCCCTTATAGCCTGGAATATATTCAGTTTGTTCCC4030872GlnLeuLeuHisValProTyrSerLeuGluTyrIleGlnPheValPro8874031CTGCTCAACCTGAAGCCCTTTGCCCAGGAGTTGCAACTCTCCGTCCTC4078888LeuLeuAsnLeuLysProPheAlaGlnGluLeuGlnLeuSerValLeu903续后页4079TTCCTGAGGACTTTCCAGTTTCTCTGCAGCCATAGCTGTCGTAATTGG4126904PheLeuArgThrPheGlnPheLeuCysSerHisSerCysArgAsnTrp9194127CTTCCTCTGGAAGGCTGGAACCACGTGGTCAAACTCCTCTGTGGCAGT4174920LeuProLeuGluGlyTrpAsnHisValValLysLeuLeuCysGlySer9354175CTGACCCGCCTCCTGGACTCAGTCAGGGCGATACAGGCAGCTGGCCCT4222936LeuThrArgLeuLeuAspSerValArgAlaIleGlnAlaAlaGlyPro9514223TGGGTTCAAGGACCAGAGCAGGACCTGACCCAGGAAGCCCTGTTTGTT4270952TrpValGlnGlyProGluGlnAspLeuThrGlnGluAlaLeuPheVal9674271TACACCCAGGTGTTCTGCCATGCTCTGCACATCATGGCCATGCTCCAC4318968TyrThrGlnValPheCysHisAlaLeuHisIleMetAlaMetLeuHis9834319CCGGAGGTCTGTGAGCCACTCTACGTTTTAGCCTTGGAAACCCTCACC4366984ProGluValCysGluProLeuTyrValLeuAlaLeuGluThrLeuThr9994367TGCTATGAGACTTTGAGCAAGACCAACCCTTCTGTCAGCTCCTTGCTC44141000CysTyrGluThrLeuSerLysThrAsnProSerValSerSerLeuLeu10154415CAGAGGGCACACGAGCAGCGCTTCTTAAAGTCCATTGCTGAGGGCATT44621016GlnArgAlaHisGluGlnArgPheLeuLysSerIleAlaGluGlyIle10314463GGCCCTGAAGAACGGCGCCAAACCCTGTTGCAGAAGATGAGCAGCTTC45101032GlyProGluGluArgArgGlnThrLeuLeuGlnLysMetSerSerPhe10474511TGACTTGGCGTGGGGAGCTGGGCCCCAACATGGCGGGTCTGCAGAAGA45581048***1048续后页4559TCAGCAGCTTCTTACCTGTGCGGGAGCGAAAAAGCTGGGCTTCAACAT46064607GGCAGGTCTGTAGGGGTCAGACCCAAGCAGCCTGGACTTTACAGTTAT46544655GTGAAACTGTCCACAAAAAGTCATGGCAATAATGGTGTAAAGAAAATA47024703GTTTCTTGGGTATTTGTAACGTACAAACTATCATAAAAATTCTCCTCT47504751TTCCCAAAAAAAAAAAAAAAAAAAAAAAA4779
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