[发明专利]玉米螟谷胱甘肽-S-转移酶基因的cDNA及其RNAi干扰工程菌的构建

权利要求书

1.玉米螟谷胱甘肽-S-转移酶基因的cDNA，其特征在于玉米螟谷胱甘肽-S-转移酶基因的cDNA全长为886bp，核苷酸序列为：

TCGCGGATCCACAGCCTACTGATGATCAGTCGATGGAAAAGTCTTACTGTGGAGTTCTGAAGCGGTGCACGCGAGTTTCAAAGCTACATCGATCGACCTTTACTACACGGCCGGGTCAGCGCCCTGTCGCCTGGTGTTACTGGTGGCTGCCGCTCTGAACCTCGAACTCAACCTTAAGCCTCTAGACTTAAGGGCTGGAGAACAGCTCACACCAGAATTTTTACAGCTGAACCCACAACACACAGTTCCCACGATCATAGACGACGGCTTTTCGCTAAATCCTCCACACACAGTTCCCACGATCGTAGACGACGGCTTTTCGCTATGGGAATCGCGCGCCATCAGCCGATACCTGGTCAACAAGTACGGAGGGGCAGCCCTGTACCCTGAGGACCCGAAGGCGAGGGCTTTGGTGGACCAGAGACTGGACTTCGACTTGGGTACACTGTACCCCAGATTTGCGACGTACTTTTATCCCCAAGTCCTCGCTGGTGCTCCTGCTGACGAAGCCTTGTTGAAGATGCTGGAAGAGGCTCTCAAGTTCCTGGACACCTTCCTCGAAGGTCAGAAGTATGCTGCGGGCTCAGAACTGACCTTGGCTGACCTGTCGCTCGTGGCTACTGTGTCTACTATTGACGCTGCTGGCATATCCATCGAATCCTACCAGAGTGTCAACAAGTGGTTCACGCTGGTCAAATCTACCGCCCCCAAATACGACGAAGCGAACGGCCAAGGCGTAGAAATGTTTAGAGCCTTCGTGGCACAGCTCAAAGCCAAGACGGAGCTGTCGTGAGACTGGTATAGTGCATAGTTTTGTATTAAGTAAATGTTTGATACATAAGTGAATTTGAAATTTAAAAGTAAAAAAAAAAAA；该基因所编码的氨基酸序列为：MetSerIleAspLeuTyrTyrThrAlaGlySerAlaProCysArgLeuValLeuLeuValAlaAlaAlaLeuAsnLeuGluLeuAsnLeuLysProLeuAspLeuArgAlaGlyGluGlnLeuThrProGluPheLeuGlnLeuAsnProGlnHisThrValProThrIleIleAspAspGlyPheSerLeuAsnProProHisThrValProThrIleValAspAspGlyPheSerLeuTrpGluSerArgAlaIleSerArgTyrLeuValAsnLysTyrGlyGlyAlaAlaLeuTyrProGluAspProLysAlaArgAlaLeuValAspGlnArgLeuAspPheAspLeuGlyThrLeuTyrProArgPheAlaThrTyrPheTyrProGlnValLeuAlaGlyAlaProAlaAspGluAlaLeuLeuLysMetLeuGluGluAlaLeuLysPheLeuAspThrPheLeuGluGlyGlnLysTyrAlaAlaGlySerGluLeuThrLeuAlaAspLeuSerLeuValAlaThrValSerThrIleAspAlaAlaGlyIleSerIleGluSerTyrGlnSerValAsnLysTrpPheThrLeuValLysSerThrAlaProLysTyrAspGluAlaAsnGly GlnGlyVal GluMetPheArgAlaPheValAlaGlnLeuLysAlaLysThrGluLeu。