[发明专利]具有蛋白酶变体的消费品

权利要求书

1.一种组合物，包含助剂材料和冷水蛋白酶变体，所述组合物为消费品。

2.如权利要求1所述的组合物，其中所述蛋白酶变体根据测试方法6具有至少1.1、至少1.2、至少1.3、至少1.4、至少1.5、至少1.6、至少1.7、至少1.8、至少1.9、至少2；1.1至约10、1.1至约8、或甚至1.1至约5的性能指数。

3.一种组合物，包含助剂材料和蛋白酶变体，其中所述蛋白酶变体包含下列各组突变中的一个或多个：G020R-N043R，N062E-A158E，S103G-A158E，S128N-A158E，A016S-A158E，V104L-A158E，E089P-A158E，L111V-A158E，T022A-A158E，S101A-A158E，L148I-A158E，P129E-A158E，T022A-E089P，A016S-E089P，N062E-E089P，N062E-E271F，A158E-E271F，R186H-E271F，P129E-E271F，L111V-E271F，Y209E-E271F，A016S-E271F，S188D-E271F，T022A-E271F，G159E-E271F，V104L-E271F，S101A-E271F，E089P-E271F，S128N-E271F，S103G-E271F，L148I-E271F，H249R-E271F，N062E-G159E，A016S-G159E，S128N-G159E，L148I-G159E，L111V-G159E，E089P-G159E，T022A-G159E，P129E-G159E，S103G-G159E，V104L-G159E，A158E-G159E，S101A-G159E，A158E-H249R，L111V-H249R，P129E-H249R，N062E-H249R，A016S-H249R，R186H-H249R，L148I-H249R，G159E-H249R，S101A-H249R，S188D-H249R，V104L-H249R，Y209E-H249R，T022A-H249R，S128N-H249R，S103G-H249R，E089P-H249R，T022A-L111V，S101A-L111V，A016S-L111V，V104L-L111V，N062E-L111V，S103G-L111V，E089P-L111V，A016S-L148I，N062E-L148I，T022A-L148I，P129E-L148I，V104L-L148I，S103G-L148I，S128N-L148I，S101A-L148I，E089P-L148I，L111V-L148I，A016S-N062E，T022A-N062E，N062E-P129E，T022A-P129E，S128N-P129E，A016S-P129E，S101A-P129E，V104L-P129E，E089P-P129E，S103G-P129E，L111V-P129E，N062E-R186H，S128N-R186H，S101A-R186H，T022A-R186H，A016S-R186H，A158E-R186H，E089P-R186H，P129E-R186H，G159E-R186H，S103G-R186H，V104L-R186H，L111V-R186H，L148I-R186H，N062E-S101A，T022A-S101A，A016S-S101A，E089P-S101A，N062E-S103G，T022A-S103G，A016S-S103G，S101A-S103G，E089P-S103G，N062E-S128N，A016S-S128N，T022A-S128N，S101A-S128N，V104L-S128N，E089P-S128N，S103G-S128N，L111V-S128N，L111V-S188D，N062E-S188D，A016S-S188D，L148I-S188D，T022A-S188D，S128N-S188D，S101A-S188D，V104L-S188D，E089P-S188D，P129E-S188D，G159E-S188D，R186H-S188D，S103G-S188D，A158E-S188D，A016S-T022A，A016S-V104L，T022A-V104L，S101A-V104L，N062E-V104L，S103G-V104L，E089P-V104L，G159E-Y209E，L111V-Y209E，S101A-Y209E，A016S-Y209E，S128N-Y209E，L148I-Y209E，P129E-Y209E，N062E-Y209E，T022A-Y209E，S103G-Y209E，A158E-Y209E，S188D-Y209E，V104L-Y209E，E089P-Y209E，R186H-Y209E，N018R-W241R，G020R-W241R，S024R-W241R，S009A-W241R，G020R-W241R，V004R-W241R，N043R-W241R，S078R-W241R，T022R-W241R，G115R-W241R，A001R-W241R，S212F-W241R，L082R-W241R，N018R-V244R，S024R-V244R，S078R-V244R，G020R-V244R，S212F-V244R，S009A-V244R，L082R-V244R，A001R-V244R，N043R-V244R，T022R-V244R，V004R-V244R，G115R-V244R，W241R-V244R，S242R-V244R，A001R-V004R，S009A-T022R，N018R-T022R，G020R-T022R，V004R-T022R，A001R-T022R，S024R-S242R，N018R-S242R，V004R-S242R，G020R-S242R，S212F-S242R，L082R-S242R，S078R-S242R，A001R-S242R，S009A-S242R，T022R-S242R，G115R-S242R，N043R-S242R，W241R-S242R，N018R-S212F，T022R-S212F，V004R-S212F，S024R-S212F，A001R-S212F，G115R-S212F，G020R-S212F，S009A-S212F，N043R-S212F，S078R-S212F，L082R-S212F，S009A-S078R，G020R-S078R，S024R-S078R，T022R-S078R，N018R-S078R，V004R-S078R，A001R-S078R，N043R-S078R，T022R-S024R，G020R-S024R，N018R-S024R，A001R-S024R，V004R-S024R，S009A-S024R，V004R-S009A，A001R-S009A，S242R-N269R，S024R-N269R，G020R-N269R，T022R-N269R，H249R-N269R，S212F-N269R，N043R-N269R，V244R-N269R，A001R-N269R，N018R-N269R，S078R-N269R，S009A-N269R，G115R-N269R，W241R-N269R，V004R-N269R，L082R-N269R，N018R-N043R，G020R-N043R，V004R-N043R，T022R-N043R，S009A-N043R，A001R-N043R，S024R-N043R，S009A-N018R，V004R-N018R，A001R-N018R，S024R-L082R，S009A-L082R，N018R-L082R，A001R-L082R，S078R-L082R，G020R-L082R，T022R-L082R，V004R-L082R，N043R-L082R，N043R-H249R，G020R-H249R，V004R-H249R，N018R-H249R，S009A-H249R，S212F-H249R，T022R-H249R，S024R-H249R，G115R-H249R，A001R-H249R，L082R-H249R，S242R-H249R，W241R-H249R，V244R-H249R，S078R-H249R，N018R-G115R，G020R-G115R，T022R-G115R，S078R-G115R，S009A-G115R，V004R-G115R，A001R-G115R，L082R-G115R，N043R-G115R，S024R-G115R，S009A-G020R，N018R-G020R，V004R-G020R，A001R-G020R，S009A-E271L，G020R-E271L，S024R-E271L，V244R-E271L，W241R-E271L，N043R-E271L，T022R-E271L，H249R-E271L，S212F-E271L，G115R-E271L，S242R-E271L，S078R-E271L，V004R-E271L，N269R-E271L，A001R-E271L，N018R-E271L和L082R-E271L，G020K-N062E，S024F-N116L，G020K-S024F，S024R-A174T，S024R-G118R，S024R-K235F，S024R-P086R，S024R-P086W，S078R-G118R，T033S-G118R，T033S-K235F，Y209A-W241R，G020R-N076D，N018R-Q245R，S024R-R045T，A232V-Q245R，G118R-A172V，G118R-A194T，I008T-S024R，K235F-N243F，N018R-S103A，N018R-V104I，P086W-G118R，P086W-N243F，P086W-Y209A，S024C-T033S，S024R-A232V，S024R-N243F，S024R-P239Q，S024R-S101G，S024R-S141G，S024R-T033S，S024R-T274I，S024R-Y209A，S078R-P086W，S101G-A232V，T033S-L148F，T033S-P086W，T033S-P201S，T033S-S078R，T033S-W241R，T033S-Y209A，A230E-H249R，A232V-H249R，G118R-K235F，N076D-Q245R，P086W-K235F，S024R-R247H，S024R-V104A，S078R-K235F，S101G-H249R，S103A-A232V，T033S-A048T，T033S-P239T，T033S-T253A，T143A-Y209A，Y209A-K235F，N018R-R045T，Y209A-N243F，S024R-A272P，S024R-R269C，S101G-V104I，V104I-A232V，N076D-H249R和S024R-N076D，G020R-N076D，S024R-R045T，A230E-H249R，N018R-R045T，N018R-Q245R，S101G-A232V，S024R-A232V，A232V-Q245R，S024R-S101G，N018R-V104I，N018R-S103A，S101G-H249R，A232V-H249R，S103A-A232V，N076D-Q245R，S101G-V104I，V104I-A232V，N076D-H249R，S024R-N076D，S024F-N116L，G020K-S024F，G020K-N062E，T033S-G118R，S024R-P086W，S024R-G118R，S024R-P086R，Y209A-W241R，S024R-W241R，S024R-K235F，G118R-Y209A，S078R-G118R，T033S-K235F，S024R-A174T，P086W-Y209A，I008T-S024R，P086W-G118R，T033S-W241R，S024R-N243F，S024R-Y209A，T033S-P086W，S024R-T033S，P086W-N243F，T033S-P201S，S024R-P239Q，S078R-P086W，K235F-N243F，G118R-A172V，T033S-L148F，T033S-S078R，T033S-N243F，S024C-T033S，G118R-A194T，T033S-Y209A，S024R-S141G，S024R-T274I，P086W-K235F，A015T-T033S，Y209A-K235F，S024R-R247H，S078R-K235F，S024R-V104A，T033S-A048T，G118R-K235F，T033S-T253A，T143A-Y209A，T033S-P239T，Y209A-N243F，S024R-A272P和S024R-R269C，并且其中相对于迟缓芽孢杆菌枯草杆菌蛋白酶GG36蛋白酶的总净电荷，所述变体的总净电荷为0、+1、+2、+3、+4、+5、-1、-2、-3、-4、或-5，并且其中所述总净电荷任选地通过一个或多个取代获得，所述取代选自：N43D，R45T，N62E，N76D，S101D，P129E，A158E，G159D，G159E，S166D，S188D，A230E，N18R，G20K，G20R，T22R，S24R，N43R，G118R，Q245R，H249R，N269R，E271F和E271L，并且其中所述蛋白酶变体的氨基酸位置根据在SEQ ID NO:2中所示的解淀粉芽孢杆菌枯草杆菌蛋白酶BPN’氨基酸序列中对应的氨基酸位置的编号而编号，所述组合物为消费品。