[发明专利]一种总胆汁酸的测定试剂

权利要求书

1.一种总胆汁酸的测定试剂，该试剂由试剂1和试剂 2组成，其特征在于，试剂1由磷酸盐缓冲液 、Thio-NAD 、EMULGEN B-66 、 CTAB 、NaCl 2-羟基吡啶-N-氧化物组成；试剂2由甘氨酸盐缓冲液、硫酸葡聚糖 、EGTA 、3α-HSD、EMULGEN B-66、 NADH2-羟基吡啶-N-氧化物组成。

2.如权利要求1所述的总胆汁酸的测定试剂，其特征在于，所述的试剂组成的各组份浓度为：

试剂1：

                   磷酸盐缓冲液                         2-6g/L

                   Thio-NAD                            1.3-1.5g/L

                  EMULGEN B-66           0.3-2ml/L

                   CTAB                                0.1-1.5g/L

                   NaCl                                 3-25g/L

                   2-羟基吡啶-N-氧化物                   0.1-1.2g/L ；

试剂2：

                   甘氨酸盐缓冲液                        10-20g/L

                   硫酸葡聚糖                            1.2-7g/L

                   EGTA                                 1-5g/L

                   3α-HSD                              10KU/L

                   EMULGEN B-66                       1-3ml/L

                   NADH                                 3g/L

                   2-羟基吡啶-N-氧化物                   0.1-1.2g/L。

3. 如权利要求1或2所述的总胆汁酸的测定试剂，其特征在于，所述的试剂组成的各组份浓度为：

试剂1：

                   磷酸盐缓冲液                         3g/L

                   Thio-NAD                            1.6g/L

                  EMULGEN B-66              1ml/L

                   CTAB                                  1g/L

                   NaCl                                    15g/L

                   2-羟基吡啶-N-氧化物                   0.3g/L ；

试剂2：

                   甘氨酸盐缓冲液                        15g/L

                   硫酸葡聚糖                              5g/L

                   EGTA                                 2g/L

                   3α-HSD                              10KU/L

                   EMULGEN B-66                    2ml/L

                   NADH                                   3g/L

                   2-羟基吡啶-N-氧化物                  0.3g/L。