[发明专利]GH9内切葡聚糖酶变体以及编码它们的多核苷酸有效
| 申请号： | 201780051024.1 | 申请日： | 2017-08-24 |
| 公开（公告）号： | CN109863244B | 公开（公告）日： | 2023-06-06 |
| 发明（设计）人： | J.E.尼尔森;L.安德森;A.斯文德森;R.N.蒙拉德;L.吉格尔;V.P.拉奥;S.达拉尔;S.V.美帕达姆;M.T.克鲁斯;K.D.兰德;P.F.詹森 | 申请（专利权）人： | 诺维信公司 |
| 主分类号： | C12N9/42 | 分类号： | C12N9/42;C12N9/88;C11D3/386 |
| 代理公司： | 北京市柳沈律师事务所 11105 | 代理人： | 张文辉 |
| 地址： | 丹麦*** | 国省代码： | 暂无信息 |
| 权利要求书： | 查看更多 | 说明书： | 查看更多 |
| 摘要： | |||
| 搜索关键词： | gh9 内切葡 聚糖 变体 以及 编码 它们 多核苷酸 | ||
1.一种内切葡聚糖酶变体，其中所述变体相对于SEQ ID NO：2的成熟多肽的改变为选自由以下组成的组的改变，其中根据SEQ ID NO: 2进行编号：
o Y934G
o Y934M
o Y934S
o Y934A
o Y934Q
o Y934N
o Y934E
o Y934W
o Y934R
o A559N+Y579W+Y934G
o A559N+Y579W+K921R+Y934G
o A559N+Y579W+S616D+K921R+Y934G
o A559N+Y579W+A651P+K921R+Y934G
o S636N+Y934G
o F638I+Y934G
o Y579W+Y934G
o P570T+Y934G
o A885F+Y934G
o A559N+Y579W+A651P+Y934G
o F638I+Y934G
o K627R+Y934G
o A448E+Y934G
o E53Y+N216R+Y934G
o P570T+Y934G
o Y55M+A559N+Y579W+A651P+Y934G
o K51Q+K627R+Y934G
o E408D+Q416S+A559N+Y579W+S616D+K921R+Y934G
o F20N+K627R+S636N+A651P+K921R+Y934G
o F20N+S616D+K627R+A651P+K921R+Y934G
o F20N+S616D+K627R+S636N+A651P+Y934G
o K627R+S636N+A651P+K921R+Y934G
o S616D+K627R+S636N+A651P+K921R+Y934G
o E408D+A559N+Y579W+S636N+K921R+Y934G
o K627R+A651P+Y934G
o S616D+K627R+K921R+Y934G
o F20N+E408D+A559N+Y579W+K921R+Y934G
o E408D+Y934G
o E408D+Q416D+A559N+Y579W+I602T+V603P+S616D+K921R+Y934G
o F20P+E53Y+Q416D+A448E+K627R+Y934G
o V56M+K627R+Y934G
o E53Y+Y55M+E408D+A559N+Y579W+S636N+K921R+Y934G
o K627R+F638I+Y934G
o K51Q+K627R+Y934G
o S616D+K627R+A651P+K921R+Y934G+G994D
o F20N+S616D+K627R+S636N+Y934G
o A448E+K627R+Y934G
o K627R+S636N+Y934G
o K627R+K921R+Y934G
o K627R+S636K+Y934G
o A559N+Y579W+K627R+Y934G
o F20N+S616D+K627R+Y934G
o F20N+E408D+A559N+Y579W+S616D+K921R+Y934G
o K627R+Q834E+Y934G
o Q416S+A448W+A559N+K627R+Y934G
o P570K+K627R+Y934G
o F20N+S616D+K627R+A651P+K921R+Y934G
o F20N+E408D+A559N+Y579W+K627R+K921R+Y934G
o K512P+K627R+Y934G
o S616D+K627R+A651P+K921R+Y934G
o S616D+K627R+S636N+A651P+K921R+Y934G
o F20P+A448E+K627R+Y934G
o K627R+S636N+A651P+K921R+Y934G
o F20N+E408D+Q416S+A559N+Y579W+S616D+K921R+Y934G
o S616D+K627R+S636N+K921R+Y934G
o F20N+K627R+Y934G
o P570T+K627R+Y934G
o F20P+E53Y+Q416D+K627R+Y934G
o F20P+E53Y+K627R+Y934G
o P570Q+K627R+Y934G
o P570K+Y579W+Y934Q
o F20N+S616D+K627R+S636N+A651P+Y934G
o S616D+K627R+S636N+A651P+Y934G
o F20N+S616D+K627R+K921R+Y934G
o P570K+Y579W+Y934G
o P570Q+K627R+Y934R
o F20P+Q416D+K627R+Y934G
o F20N+K627R+S636N+K921R+Y934G
o F20N+K627R+S636N+A651P+K921R+Y934G
o F20N+S616D+K627R+S636N+S642N+K921R+Y934G
o E408D+A559N+Y579W+S616D+K921R+Y934R+A937E+K939V
o E408D+Q416S+A559N+Y579W+S616D+K921R+Y934G
o E408D+Y934G
o F20N+E408D+A559N+Y579W+K921R+Y934G+T883R
o E408D+A559N+Y579W+S616D+K921R+Y934R+A937E+K939V
o E408D+A559N+Y579W+I602T+V603P+S616D+K921R+Y934G+A885Q
o E408D+A559N+Y579W+S616D+K921R+Y934G+A937E+K939V
o F20N+E408D+Q416S+A559N+Y579W+S616D+K921R+Y934G
o A559N+Y579W+P570K+Y934R+Q416S
o E408D+A559N+Y579W+I602T+V603P+S616D+K921R+Y934G+Y884H
o E408D+A559N+Y579W+S616D+K921R+Y934G
o E408D+Q416D+A559N+Y579W+K921R+Y934G
o A559N+Y579W+P570K+Y934R+Q416D
o A559N+Y579W+P570K+Y934R+F20P
o E408D+A559N+Y579W+I602T+V603P+S616D+K921R+Y934G+A885F
o E408D+A559N+Y579W+I602T+V603P+S616D+K921R+Y934G+Q834E
o E408D+Q416D+A559N+Y579W+K627R+K921R+Y934G
o A559N+Y579W+P570K+Y934R+K627R
o E53Y+Y55M+E408D+A559N+Y579W+S636N+K921R+Y934G
o E408D+A559N+Y579W+K921R+Y934G+T697G+F20N
o E408D+Q416D+A559N+P570R+Y579W+K921R+Y934G
o F20N+E408D+A559N+Y579W+K921R+Y934G
o A559N+Y579W+P570K+Y934R+A651P
o E408D+A559N+P570Q+Y579W+K921R+Y934G
o F20N+E408D+A559N+Y579W+K921R+Y934G+T999R
o P570K+Y579W+Q416D+S17A+F20P+Y934R
o E408D+A559N+Y579W+I602T+V603P+S616D+K921R+Y934G+S636K
o E408D+A559N+Y579W+S636N+A651P+K921R+Y934G
o E408D+A559N+Y579W+S636N+K921R+Y934G
o E408D+Q416D+A559N+Y579W+I602T+V603P+S616D+K921R+Y934G+A651P+A885F
o E408D+A559N+Y579W+I602T+V603P+S616D+K921R+Y934G+S17A
o E408D+A559N+Y579W+I602T+V603P+S616D+K921R+Y934G
o F20N+E408D+A559N+Y579W+K627R+K921R+Y934G
o A559N+Y579W+P570K+Y934R+E408D
o E408D+A559N+Y579W+K921R+Y934G+A937E+K939V
o F20N+E408D+A559N+Y579W+K921R+Y934G+I302D
o F20P+E408D+Q416S+A559N+S560P+P570R+Y579W+K627R+K921R+Y934R
o F20P+E408D+A559N+Y579W+K921R+Y934G
o E408D+A559N+Y579W+I602T+V603P+S616D+K921R+Y934G+A937E+K939V
o E408D+A559N+Y579W+I602T+V603P+S616D+K921R+Y934R+A937E+K939V
o F20N+E408D+A559N+Y579W+K921R+Y934G+R880K
o A559N+Y579W+P570K+Y934R+E408N
o F20P+E408D+Q416D+A559N+S560P+P570R+Y579W+K627R+S636N+F638I+K921R+Y934R
o E408D+A559N+Y579W+I602T+V603P+S616D+K921R+N933S+Y934R+A937E+V938I+K939V+N941S+A942P
o A559N+Y579W+P570K+Y934R+Q683E
o F20P+E408D+Q416D+A559N+S560P+P570Q+Y579W+K627R+K921R+Y934G
o E408D+Q416D+A559N+Y579W+I602T+V603P+S616D+A651P+K921R+Y934G
o E408D+A559N+Y579W+S636N+Q683E+K921R+Y934G
o F20P+E408D+Q416S+A559N+S560P+P570R+Y579W+K627R+S636N+F638I+K921R+Y934G
o F20N+E408D+A559N+Y579W+K921R+Y934G+T697G+Y55M
o E408D+Q416D+A559N+Y579W+I602T+V603P+S616D+K921R+Y934G
o S17A+F20P+E408D+Y579W+I602T+A651P+F906A+Y934G
o F20N+E408D+A559N+Y579W+K921R+Y934G+T887S
o E408D+Y579W+I602T+F638N+A651P+R880K+K921R+Y934G
o E408D+Q416D+A559N+Y579W+I602T+V603P+S616D+K921R+Y934G+A651P+Y884H
o F20N+E408D+A559N+Y579W+K921R+Y934G+V756Y
o S17A+F20P+E408D+Y579W+I602T+A651P+F906A+Y934G+S636K
o F20N+E408D+A559N+Y579W+K921R+Y934G+T697G+T887K
o F20N+E408D+A559N+Y579W+K921R+Y934G+T697G+A885Q
o S17A+F20P+E408D+Y579W+I602T+A651P+F906A+Y934G+V881Q
o I302D+E408D+Q416S+Y579W+I602T+A651P+R880K+Y934Q
o S17A+F20P+E408D+Y579W+I602T+A651P+F906A+Y934G+L888M
o S17A+F20P+E408D+Y579W+I602T+A651P+F906A+Y934G+S560P
o E408D+A559N+Y579W+S616D+K921R+T697G+Y934G
o S17A+F20P+E408D+Y579W+I602T+A651P+F906A+Y934G+T887K
o A559N+Y579W+A688G+V756Y+K921R+Y934G
o E408D+S636N+A651P+V756H+Y934Q
o S17A+F20P+E408D+Y579W+I602T+A651P+F906A+Y934G+S636N
o E408D+Q416D+A559N+Y579W+I602T+V603P+S616D+K921R+Y934G+A651P+T887S
o E408D+A559N+Y579W+K921R+Y934R+A937E+K939V
o E408D+A559N+Y579W+K921R+Y934R+A937E+V938I+K939V+N941S+A942P
o F20N+E408D+A559N+Y579W+Q683E+K921R+Y934G
o E408D+Y579W+A651P+I602T+Y934Q
o F20N+E408D+A559N+Y579W+T697G+K921R+Y934G
o E408D+A559N+Y579W+I602T+V603P+S616D+K921R+Y934G+Q683E
o E408D+Y579W+I602T+F638N+A651P+R880K+K921R+Y934G+K51Q
o F20N+E408D+A559N+Y579W+K921R+Y934G+T697G+K512P
o E408D+Y579W+I602T+F638N+A651P+N848D+R880K+K921R+Y934G
o N18G+N189K+E408D+A559N+Y579W+A688G+T697G+V756Y+K921R+Y934G
o E408D+Y579W+I602T+F638N+A651P+V756Y+R880K+K921R+Y934G+P570R
o S313D+E408D+Y579W+I602T+F638N+A651P+R880K+K921R+Y934G
o E408D+Y579W+I602T+F638N+A651P+R880K+N905D+K921R+Y934G+A283D
o E408D+Y579W+I602T+F638N+A651P+R880K+N905D+K921R+Y934G+A885Q
o E408D+Y579W+I602T+F638N+A651P+R880K+N905D+K921R+Y934G
o S17A+F20P+E408D+Y579W+I602T+A651P+F906A+Y934G+S313D
o E408D+Y579W+I602T+F638N+A651P+R880K+K921R+Y934G+Y55D
o E408D+Y579W+I602T+F638N+A651P+R880K+K921R+Y934G+N848D
o S313D+I302D+E408D+Y579W+I602T+F638N+A651P+R880K+K921R+Y934G
o F20N+A346D+E408D+K512P+A559N+Y579W+I602T+S636N+A651P+T697G+V756Y+A824D+A885N+K921R+Y934G
o F20N+E408D+Q489P+K512P+A559N+Y579W+I602T+A651P+T697G+V756Y+A885N+K921R+Y934G+A937E
o E408D+Y579W+I602T+F638N+A651P+R880K+K921R+Y934G+V756Y
o E408D+Y579W+I602T+F638N+A651P+R880K+N905D+K921R+Y934G+N848D
o F20N+E408D+K512P+A559N+Y579W+T697G+K921R+Y934G+A651P
o S17A+F20P+E408D+Y579W+I602T+A651P+F906A+Y934G+A688G
o E408D+Y579W+I602T+F638N+A651P+R880K+N905D+K921R+Y934G+A885N
o E408D+Y579W+I602T+F638N+A651P+V756Y+R880K+K921R+Y934G
o E408D+Y579W+I602T+F638N+A651P+A688G+K720H+R880K+K921R+Y934G
o K51Q+E408D+Y579W+I602T+F638N+A651P+R880K+K921R+Y934G
o Y55M+A283D+S313D+E408D+Y579W+I602T+F638N+A651P+K720H+V756Y+N848D+A869V+R880K+Q912V+K921R+Y934R
o S313D+A346D+E408D+Y579W+I602T+F638N+A651P+T697G+N848D+R880K+A885N+K921R+Y934G
o E408D+Y579W+I602T+F638N+A651P+R880K+N905D+K921R+Y934G+A688G
o E408D+Y579W+I602T+F638N+A651P+V756Y+R880K+K921R+Y934G+N848D
o S17A+F20P+E408D+Y579W+I602T+A651P+F906A+Y934G+T697G
o Y55M+A283D+S313D+E408D+Y579W+I602T+F638N+A651P+K720H+V756Y+N848D+A869V+R880K+N905D+K921R+Y934R
o S313D+E408D+S560P+Y579W+I602T+F638N+A651P+W719R+V756Y+T825G+N848D+A869V+D870V+R880K+N905D+K921R+Y934G
o S313D+E408D+S560P+Y579W+I602T+F638N+A651P+W719R+V756Y+N848D+A869V+D870V+R880K+K921R+Y934G
o E408D+Y579W+I602T+F638N+A651P+V756Y+R880K+K921R+Y934G+S313D
o S17A+F20P+N216D+I302D+E408D+Q416S+Y579W+I602T+A651P+W719R+T883R+F906A+Y934G+Q956Y
o S313D+E408D+Y579W+I602T+F638N+A651P+T697G+R880K+K921R+Y934G
o S313D+E408D+Y579W+I602T+F638N+A651P+T697G+R880K+K921R+Y934G
o S313D+E408D+S560P+Y579W+I602T+F638N+A651P+W719R+V756Y+N848D+A869V+D870V+R880K+N905D+K921R+Y934G
o S313D+E408D+Y579W+I602T+F638N+A651P+T697G+R880K+K921R+Y934G
o Y55M+A283D+S313D+E408D+Y579W+I602T+F638N+A651P+W719R+V756Y+N848D+A869V+R880K+K921R+Y934R
o F20N+E408D+K512P+A559N+Y579W+I602T+A651P+T697G+V756Y+K921R+Y934G
o Y55M+A283D+S313D+E408D+Y579W+I602T+F638N+A651P+W719R+V756Y+N848D+A869V+R880K+K921R+Y934R
o A283D+S313D+E408D+A559P+Y579W+I602T+F638N+A651P+W719R+V756Y+N848D+A869V+D870V+R880K+N905D+K921R+Y934G
o S313D+E408D+S560P+Y579W+I602T+F638N+A651P+W719R+V756Y+N848D+A869V+D870V+R880K+N905D+K921R+Y934G
o S17A+F20P+N216D+A283D+H311N+E408D+Y579W+I602T+A651P+A688G+T883R+F906A+Y934G+Q956Y
o Y55M+A283D+S313D+E408D+A559P+Y579W+I602T+F638N+A651P+T697G+W719R+N848D+R880K+K921R+Y934G+A937E
o S17A+F20P+N216D+A283D+H311N+E408D+S560P+Y579W+I602T+A651P+A688G+T883R+F906A+Q912V+Y934G+Q956Y
o Y55M+A283D+S313D+E408D+Y579W+I602T+F638N+A651P+W719R+V756Y+N848D+A869V+R880K+N905D+K921R+Y934R
o F20N+Y55M+H311N+E408D+K512P+A559N+Y579W+A651P+T697G+N848D+K921R+Y934G
o Y55M+A283D+S313D+E408D+Y579W+I602T+F638N+A651P+W719R+V756Y+N848D+A869V+R880K+K921R+Y934R
o Y55M+A283D+I302D+S313D+E408D+Y579W+I602T+F638N+A651P+W719R+V756Y+N848D+A869V+R880K+K921R+Y934R
o Y55M+A283D+S313D+E408D+Y579W+I602T+F638N+A651P+W719R+V756Y+N848D+A869V+R880K+A885N+K921R+Y934R
o Y55M+A283D+S313D+E408D+Y579W+I602T+F638N+A651P+W719R+V756Y+N848D+A869V+R880K+N905D+K921R+Y934R
o Y55M+A283D+I302D+S313D+E408D+Y579W+I602T+F638N+A651P+W719R+V756Y+N848D+A869V+R880K+K921R+Y934R
o S313D+E408D+Y579W+I602T+F638N+A651P+T697G+N848D+R880K+K921R+Y934G
o Y55M+A283D+S313D+E408D+Y579W+I602T+F638N+A651P+W719R+V756Y+N848D+A869V+R880K+N905D+K921R+Y934R
o Y55M+A283D+I302D+S313D+E408D+Y579W+I602T+F638N+A651P+W719R+V756Y+N848D+A869V+R880K+K921R+Y934R
o Y55M+A283D+I302D+S313D+E408D+Y579W+I602T+F638N+A651P+W719R+V756Y+N848D+A869V+R880K+N905D+K921R+Y934R
o A283D+S313D+E408D+Y579W+I602T+F638N+A651P+T697G+K720H+V756Y+N848D+A869V+R880K+A885Q+K921R+Y934G
o Y55M+A283D+I302D+S313D+E408D+Y579W+I602T+F638N+A651P+W719R+V756Y+N848D+A869V+R880K+K921R+Y934R
o Y55M+A283D+S313D+E408D+Y579W+I602T+F638N+A651P+K720H+V756Y+N848D+A869V+R880K+K921R+Y934R
o Y55M+A283D+I302D+S313D+E408D+Y579W+I602T+F638N+A651P+W719R+V756Y+N848D+A869V+R880K+A885N+N905D+K921R+Y934R
o Y55M+A283D+S313D+E408D+Y579W+I602T+F638N+A651P+Y690F+T697G+W719R+V756Y+R880K+K921R+Y934G+A937E
o Y55M+A283D+I302D+S313D+A346D+E408D+Q489P+A559P+Y579W+I602T+V603P+F638N+A651P+T697G+W719R+K744H+A824D+N848D+R880K+V881T+F906A+Q912V+K921R+Y934G+A937E+K948R+Q956Y+F1048W
o N216D+S313D+E408D+D476R+Y579W+I602T+V603P+F638N+A651P+A688G+T697G+W719R+V756H+T825G+N833D+A869V+R880K+T887K+K921R+S928D+Y934G+N941S+K948R
o N216Q+S313D+E408D+D476R+Y579W+I602T+F638N+A651P+T697G+W719R+R880K+T887K+K921R+Y934G
o S17A+F20P+S313D+E408D+Y579W+I602T+A651P+T697G+W719R+N848D+T883R+F906A+Y934G+Q956Y+T999R
o N216D+S313D+E408D+D476R+A564E+Y579W+I602T+F638N+A651P+Y690F+T697G+W719R+V756H+N833D+A869V+R880K+V881T+T887K+K921R+S928D+Y934G+T999R
o Y55M+A283D+I302D+S313D+A386P+E408D+Q489P+A559P+Y579W+I602T+V603P+F638N+A651P+T697G+W719R+Q834E+N848D+R880K+V881T+T892P+F906A+Q912V+K921R+Y934G+A937E+F1048W
o Y55M+A283D+I302D+S313D+E408D+A559P+Y579W+I602T+V603P+F638N+A651P+T697G+W719R+N833D+N848D+R880K+V881T+F906A+Q912V+K921R+Y934G+A937E+N941S+T999R
o Y55M+A283D+I302D+S313D+E408D+A559P+Y579W+I602T+V603P+F638N+A651P+T697G+W719R+N848D+A869V+R880K+V881T+F906A+Q912V+K921R+Y934G+A937E
o N216D+S313D+E408D+D476R+Y579W+I602T+V603P+F638N+A651P+A688G+T697G+W719R+V756H+R880K+T887K+K921R+S928D+Y934G+K948R
o F20P+Q289E+I302D+S313D+E408D+D476R+Q489P+A559P+Y579W+I602T+S636N+T697G+W719R+V756Y+A824D+N848D+R880K+V881Q+T887K+N905D+F906A+S928D+Y934G+T999R+A1037E+F1048W
o N216D+S313D+A346D+E408D+D476R+Q489P+A559P+Y579W+I602T+F638N+A651P+A688G+T697G+W719R+V756H+R880K+T887K+K921R+S928D+Y934G
o N216D+S313D+E408D+D476R+Q489P+A559P+Y579W+I602T+F638N+A651P+A688G+T697G+W719R+V756H+Q834E+R880K+T887K+T892P+K921R+S928D+Y934G。
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