[发明专利]TRIM63基因片段作为母猪肢蹄骨密度SNP分子标记

权利要求书

1.一种SNP分子标记在母猪肢蹄骨密度辅助选择中的应用，所述母猪的品种为纯种大白猪和纯种长白猪，所述SNP分子标记的核苷酸序列如下所述：

GATGTTGATTTTCTCGTCTTCGTGCTCCTTGCACATGGGGTGGCTGCCCTTCTGCAGGGGCCGACTGCAGAGGAGAAGAGTCATGAGTCACGAGGTGAGGATCTCAAGTTCCCGTCTGCCCCCCGCGCCAGCCCCAGGAGGCCACCACAGACCTGTGACTCCAGTTCTCAGGGCTTGCCTGTCCCCATCCTTTTCTTTTCATCCTCTGAGGACCCAAGACCATGAGAGCCAATGACACCAAGGCTAGGATTCAAACTCCAGTGATGGAGTTGAACTTGGGCCCTGATAGCACAGGATGTGGGAAGGGCTGTAGGATCCCAAAGTCAATTTGAGACATTTTCAAGAAGAGGAAACTGGGTTCCAGAAAGGTGAATTGACTTGCCCAAGGTCACCCAGCAAGTCAGGGCAAGGGAGGCTAGAGTTCATTTGCTCTAATTATAGTCTCCATAGCTCTTGGTTACAGCAGGCCGGGCGCTGAGCAAAGTAATTTATACTCATGGCCTCACTGAATTCTATATTTAATCCACTAGACTGTAATCTCCATCAAAGCAGGGAAACTTGCCTGTCCCTGAATCTGCAGTGTACAGTGCCTGGCGCAGAGAAGGTGGTCATTAAATACAGGCAGAAAAATAAATGGGGAAGCATGCCATGAGGGTGCCCTGAGGCTCCGAGAAGGAGGTGGTATGTGTGCCCCAAGTCACACAGCTAGAACATGGGCCATCAGGGCGTCCAACAGCCCAGCACAAGCCTTGACTTCTAGTCCAGAAATTTTTTCCAGGGTCCAGAATCACTTCTTTGCCTTTTTTCCCAAATACACACGTTTTTTGGTGTGTGTCTGCTGCATATGGAAGTTCCCAGGCCAGGGATCAAACTTGAGCCATAGCAGTAACCTGAGCCACAGCAGTGACAATGCCGAGTCCTTAAATGCTAGGGCAGGGAACTCCCCAAATACACACTCTTAGAATGACTTTCTCCCTCTGTCTCTCTCTTTGCTTCAGGCTTCTGGCATGTTCTTCTGTGCTTCTCTCAGGGTCACTTCCTACCTGAGATTCAGTTTTTGGAGTCTAGGTCTTACCGCTCCAGCTATACATTATACTCTCATTTAGAGCCAATAGCATGTCTACACCATCTCTGAGTTCCCAATCTCATAAAGGGTGAATCGTCCCCATCTGTGTCAGTGCCTCACCTCCAAACAGCCTTGCATGACTGATCTCACCCTGATCACAGACCTCAAGGTCTTAAATAAGCCCCTTTCCAGACTTGTCTTTTTGCATCTTTCAGCCCACCCCACCCCCACCCCATGCCGGCAGGGCTGTGCCTTTGCCACCTGTCTCTACCCCCGGGAAGGGATCTCGAGGAGGGCAGTAGGTACAGAGGTGAGGCAAGATCCTGGGGGTCTGGTGCCTCTGTCCTCCACCATCCCAGATGCCATCCTCCCAATCCCAGGTGCCAGCATCTGGGATCAAGTCCAGATTGATCTGCCACTTATTTACTGGGAACTTGGACAAGTGATTTTACCTCTCTGAGCCTTGCTTTTATCCCAGAGCTAGTGGGATCCTGGCAGGAGTTGTCTGTAATGATTGCATAGTGCCTGGCACAGAGCAGATCCTCTGCAACTATCATCATCCTTCAACTGTGTAGAAAGACCTCGAAGGGTTCAAACACTAGGTTCTTCCTAGGTCAACACTGGGTGGGCTAATATTTTGGTACAATATTGGTTTTATTATGATTATTATTATCACGATCGACAATATAATTGAATTTTTGCAGTGTTGAACTCTAGCAACCTGACAGGCAGTAGAAGTGACAAATGTCCACTATGGAGTCAATCAGACAGGCTTCTTCTCGGCTTCGCCACTGGCTAGCTGAGTGGCCTCGGCCAAACGTCTGCCTGCAGAAAGGCACTTGGTGATGGTTTGGGACCACAAGGGGCATGAGGTTAGAGTCTGGAGGTCCTGATTGTAAAGCTATCTCCCTGGTAGCTCTGTCTCTCCCCCTGGATCTGAGGCCCAACACTGCACAGCCTCCACCACTCACAAATTCTGCCTGAGAATGAGCTGCTGTTTCACAAGCAAGGAGACTTGGAACCGGATGGATGGGGCCGAGCAATTCTGCTTCTCGGCTGGGAAAGCTTGGGTAATTGACTTCACCTCTTTGAGCCTCAGCTTGCTCATCCGTAAAGTAGGGATGATGGTCCACAGGACTGCTCTGAAGCAGCAGCGAGAAGCAGCCCCAGTGTCTGGCGCTGTAGTTATCACCTCGGGGAAACTGGCTCTGTTCTGTGTGCTTTCTTGCTTATCRGGGCCACTGAGCTGGCCCCAGTGCCTCTGTCTCCCTCTCGCATCTCATCCTTCCCCTGGTTTCTGTCTCAGGAGGGCAGCTTAATGTCTGGGTCCCCTCCCATTCACCGAGTGACACCGAGAGCCAGGGTTCAGGGATAAGATGAGCTGCTGTTCCTGCCTGAGCAAGAGGGCTGGCTCCGGGCTGGATGAAGGGGAACAGAGCCTCGGGGAGCCAGGCTCAGAGGACAGGCTGGGGAGGAAGGACGGCCTCCCAGCCGCTGGAAAACCGACCTGGAGCACTCCTGCTTGTAGATGTCAATGATGTTCTCCACCAGCAGATTCCTCTGCAGGCCGTACACTCCGTGACGGTCCATGATCACCTCATGGCGGCAGGAGGGACAGCGGAAACGGCCTCCGGACATGGACACCGAGCCACTCCGGCTGGTCCAGTAGGGATTTGCAGCCTGCTGGTGACAAGGGCAGGGGTGAGGCCTGGGCTCCCTCCTCAGTCCCTTCCCCTTTCTGATCTCCCACCAGCCTTCTCCTGCTTACCGCCAAGAATTCTCATCAAGTCACAGGACTTAAAGCTCCCCGTCCTGGGAGGATATGTGAAAAAGAATGTATGTATATGTATATATGTAGTATGACTGGGTCACTTTGCTGTACAGCAGAAACTGGCACAACATTATAAATTAACTATACTTTAATTTAAAAAAAGTGAGGTGGTTCCGGTTGTGGTGCAGCTGTAATGAACCCGACTAGTATTGATTAGTATCCATGAGGACAGGGTTCGATCCCTGGCCTCACTCAGTGGATTAAGGAGCTTGCATTGCCATGAGCTGTGGTGTAGGTCGCAGATGAGGCTTGGATCCCGTGTTGCTGTGGCTGTAGTGTAGGCCAGAAGCTGCAGCTCCAATTCAGCCCCTAGCCTGGGAACTTCCATATGCCATGGGAGTGGCCCTAAAAAGCAAAGAAAAAAAAAAGGTAAATCTCCCCATCCTGAGATGAGAGAACAGTCACTTACACAGGGAGCATCTGAGGAGTGACTAAGCAATCAACAGGTTTGGAGGTCAACAGACCTAGGTTCAAGTCCCAGTAATTCTCTCAATGACTTTGAGCGAGCAACCTTCTCTCTCTAAGCCTCAGTTTGCCCATTCATTAAATGGGCCTATAATTAGAGCCAGCTCATAGGGCTGCTACTTTATTATTTATCTATTTCTTTATTTTAGCCATGGCCACTGCCTGTGGAAGTTCCTGGGCCAGAGATCAAACCCACACCATAGCAGTGACAACGCTGAATCCTTAACCACCTGGCCACCAGGGAACTCCCTAGGGCTGCAGCTTTAAAAACAAAGTAGTGAGAGGACATGTTGTGAAGTGCTTCTGGGGGAATCAGGAGCACTTTCTTAGGGAGAGGAAGCCTCTCCTGCCCCATAGTATAGGAAACAGCTTCAAAAAGGAAGATGCCTCTACTGCCCAGGGCCTCTCGGCCACCGGGCCACCCCTCTCCTAAAGCTCTGGGCCCCCTGCCACCTCCCAGCTGGCTCCAGCCCCTGGGGTCTGGTTTCTCTCTCCTCTCTGCTTGCCTCTCCCACTTATCGCTTGAGGCATTTGACTCCTGATTGGAGAGTCAACGGCTGCTCAGGGCAGGGGTGGGGTAGCTGCAGGCAGGGTCGGGAGGGGACATGTGCGTCATGGAGAAGGAATGGGGCAGCCAGAGCTGCCTCCCTAGCCCCCTCAGCCCCTTCCAGAAGCCTCCGGAGCAGCCATTCACTTCAGCAGGGAAACAACCCCAGGCTGGAAGGGGCCCGGGCTAAGCAAGAAGTGCTCTTTGCATTTCCATCACGGGTAAACTGAGTAGCCACTTGGGACCCCCGGACTAGGCCATGCCCAGCACTGACCTGGAAGATGTCGTTGGCACACTTTCGGCAGAGGTTGTGCTGACACGGCAAGATGACCACCGGCTTGGTAAACATCTCCAGGCAGATGGGGCAGATCAGCTGCTTCTCCAGGTTCTCCATGGGGTTCCCATCCTGGATCAGGCTTGACTTATAATCCATATTGTTGGGAGACGTGGGGACCCGCCTGGCTGCCTCCTTCACCTGCTCTAAGCAAACCTGGGGTCTTGCCTTTGTCACAAAATACCCACCCGGAGCCTCTTTGCCCTCTTCTCCTGGCGCCTGAATTCTGTCTTGGTCTGAGGCCCCTCTGATATTTATAGCTGAACCCCGGTCACATGAGCCCCAGGAGGGGACCAGCTGAGCATTCCGGTGCCAAGTGGCTGGCGAGGCTTGAGTTTCCTCCAAGCCTGAAAGACTG，

上述序列的第2301位碱基处的R是A或G，导致产生多态性，基因型为AG的个体肢蹄骨密度显著高于AA个体的肢蹄骨密度。