[发明专利]一种籼稻花药培养分化改良培养基

权利要求书

1.一种籼稻花药培养分化改良培养基，其特征在于，培养基的配方如下：KNO₃ 1850～1950mg/L、NH₄NO₃ 1600～1700mg/L、KH₂PO₄ 165～175mg/L、CaCl₂·2H₂O 440～470mg/L、MgSO₄·7H₂O 365～370mg/L、EDTA二钠37～38mg/L、FeSO₄·7H₂O 27～28mg/L、MnSO₄·4H₂O22～23mg/L、ZnSO₄·7H₂O 8.5～9.5mg/L、H₃BO₃ 5.2～6.8mg/L、KI 0.6～0.9mg/L、CuSO₄·5H₂O 0.02～0.03mg/L、Na₂MoO₄·2H₂O 0.15～0.33mg/L、CoCl₂·6H₂O 0.01～0.03mg/L、肌醇95～105mg/L、甘氨酸1.9～2.5mg/L、半胱氨酸130～250mg/L、叶酸0.4～0.55mg/L、精氨酸1.0～2.0mg/L、谷氨酸0.6～1.2mg/L、维生素B₁ 0.2～0.4mg/L、维生素B₆ 0.5～0.8mg/L、维生素E 0.1～1mg/L、维生素C 1.2～2.4mg/L、赤霉酸0.05～0.5mg/L、对氨基苯甲酸0.1～1mg/L、蔗糖30～50g/L、泛酸钙1～9mg/L、核黄素0.2～1.4mg/L、植物凝胶6～14g/L、NAA1.58～2.58mg/L、KT 0.55～3.5mg/L、6-BA 1.8～3.8mg/L、2,4-D 2.5～5mg/L、活性炭1～5mg/L酶水解酪蛋白0.9～1.1g/L，PH 5.6～6.1。

2.根据权利要求1所述的一种籼稻花药培养分化改良培养基，其特征在于:KNO₃1950mg/L、NH₄NO₃ 1650mg/L、KH₂PO₄ 171mg/L、CaCl₂·2H₂O 455mg/L、MgSO₄·7H₂O 370mg/L、EDTA二钠37.5mg/L、FeSO₄·7H₂O 27.5mg/L、MnSO₄·4H₂O 22.5mg/L、ZnSO₄·7H₂O 9mg/L、H₃BO₃ 6.3mg/L、KI 0.83mg/L、CuSO₄·5H₂O 0.025mg/L、Na₂MoO₄·2H₂O 0.25mg/L、CoCl₂·6H₂O 0.025mg/L、肌醇100mg/L、甘氨酸2.0mg/L、半胱氨酸190mg/L、叶酸0.45mg/L、精氨酸1.5mg/L、谷氨酸0.9mg/L、维生素B₁ 0.3mg/L、维生素B₆ 0.6mg/L、维生素E 0.5mg/L、维生素C 1.8mg/L、赤霉酸0.25mg/L、对氨基苯甲酸0.5mg/L、蔗糖45g/L、泛酸钙5mg/L、核黄素0.8mg/L、植物凝胶10g/L、NAA 2.06mg/L、KT 2.06mg/L、6-BA 2.81mg/L、2,4-D 3.75mg/L、活性炭2mg/L酶水解酪蛋白1.0g/L，PH 5.8。

3.根据权利要求1所述的一种籼稻花药培养分化改良培养基，其特征在于，所述植物激素比例为2,4-D：NAA：KT：6-BA＝2:1.1:1.1:1.5。

4.根据权利要求1或2中所述的籼稻花药培养分化改良培养基，其特征在于，蔗糖的工作浓度为40～45g/L。