[发明专利]呼吸道病原多重RPA检测用引物群、检测试剂及试剂盒

权利要求书

1.呼吸道病原多重RPA检测用RPA引物群，其特征在于：该引物群由15 对引物组成，所述的15对引物的核苷酸序列分别为：

人巨细胞病毒HCMV

HCMV-F：5'biotin–CGGTACGGGCTGCAGGTAAAGTGCGATCAAGAACG 3'、

HCMV-R：5'NH₂-C6-TTTTTTTTTCGTGCTTTTTAGCCTCTTTTTTTTTTTGCAGCTGCG

TCACGGGTCTAGCCGGCCATAATCAC 3'；

腺病毒ADV

ADV-F：5'biotin –CTCGGAGTACCTGAGCCCCGGGCTGGTGCAGTTCG 3'、

ADV-R：5'NH₂-C6-TTTTTTTTTTCGTCGTGCGTAGGCGCTTTTTTTTTTACCGTGGGGTT

TCTAAACTTGTTATTCAGGCTGA 3'；

副流感病毒1 型PIV1

PIV1-F：5'biotin –AAATGAGACTACAGATTACTCGAGTGAAGGTATA 3'、

PIV1-R：5'NH₂-C6-TTTTTTTTTTTAGAAAAAGGATGGTCATTTTTTTTTAAGTTATATCT

TCATTTTTGTATCGATGAGATT 3'；

副流感病毒2 型PIV2

PIV2-F：5'biotin –CAACTGATCTAGCTGAACTGAGACTTGCTTTCTA 3'、

PIV2-R：5'NH₂-C6-TTTTTTTTTTCAACTGCAGGATTGATTTTTTTTTTTTTGGCCCAT

TGCCCTGTTGTATTTGGAAGAGATAT 3'；

副流感病毒3 型PIV3

PIV3-F1：5'biotin –ACAGATAGGGATAATAACTGTAAACTCAGACT 3'、

PIV3-R：5'NH₂-C6-TTTTTTTTTTAGTGCTAGAGAACATGTTTTTTTTTTCTTCCTATTGT

CATTTATGTTAAAAGTATGAGA 3'；

呼吸道合胞病毒RSV

RSV-F：5'biotin –TCCTCACTTCTCCAGTGTAGTATTAGGCAATGCT 3'、

RSV-R：5'NH₂-C6-TTTTTTTTTTAATCACACCATTTTCTTTTTTTTTTTTTGAGTTGTTCA

GCATATGCCTTTGCTGCATCATATA 3'；

金黄色葡萄球菌SA

SA-F：5'biotin –GAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTT 3'、

SA-R：5'NH₂-C6-TTTTTTTTTCAACAGTACCACGACCAGTTTTTTTTTTTGAATACGTC

CTCAACTGGCATCATGAATGGTTTGT 3'；

肺炎链球菌SP

SP-F：biotin – CGTGCTCCGATGACTTATAGTATTGATTTGCCTGGTT 3'、

SP-R：5'NH₂-C6-TTTTTTTTTTCCAACAAATCGTTTACCTTTTTTTTTTTCCGCGAACA

CTTGAATTGCTGGGGTCTTCCA 3'；

肺炎支原体MP

MP-F：5'biotin– GCGCTAAGGGCATCACTGCCGGCAGTGGCAGTCAAC 3'、

MP-R：5'NH₂-C6-TTTTTTTTTCGAGGTCGTATAAGGCTTTTTTTTTTTCGCCCGGCGAG

GTCATACCGGCGTAACGCAAAGGTGG 3'；

肺炎衣原体CP

CP-F：5'biotin–GCCATTTTATCTCCAACTTGAAGTTTTCTCTTAGA 3'、

CP-R：5'NH₂-C6-TTTTTTTTTTTATTTCCGTGTCGTCCTTTTTTTTTTTGCCATTTTATCT

CCAACTTGAAGTTTTCTCTTAGA 3'；

肺炎克雷伯菌KP

KP-F：5'biotin–CTGGATCTGACCCTGCAGTACCAGGGTAAAAAC 3'、

KP-R：5'NH₂-C6-TTTTTTTTTGCTCGCTGATTATCATGTTTTTTTTTTTGCGGTTGGCC

GATCATGTTTGGCCTGATCCTCTCTGCG 3'；

鲍曼不动杆菌AB

AB-F：5'biotin–AGCTCAAGTCCAATACGACGAGCTAAATCTTGAT 3'、

AB-R：5'NH₂-C6-TTTTTTTTTATTTAAGTGGGATGGTTTTTTTTTTTTTATTCCCAGAATG

GGAAAAGGACATGACCCTAGGC 3'；

铜绿假单胞菌PA

PA-F：5'biotin–CTTCAACGAGAACCTGCTCTGCTTCACCAACAAC、

PA-R：5'NH₂-C6-TTTTTTTTTTCGGCCTCGATGTAGTTGTTTTTTTTTTTCAGGTTACG

CGTCAGCGCCGAACGGAAACCGGCC 3'；

嗜麦芽窄食单胞菌SM

SM-F：5'biotin–AGCGACGAGCGAACGCGGACTAGCCCTTAAGCTGAT 3'、

SM-R：5'NH₂-C6-TTTTTTTTTTCTCGTCTTCACTGAAATGTTTTTTTTTTTCCCTTTCA

GATACAGGGCTATCACCTTCTATGGCCAC 3'；

嗜肺军团菌LP

LP-F：5'biotin–GCAATGGCTGCAACCGATGCCACATCATTAGCT 3'

LP-R：5'NH₂-C6-TTTTTTTTTTACATCTATGCCTTGATTTTTTTTTTTCCCCAAATCGG

CACCAATGCTATAAGACAACTTA 3'。