[发明专利]一种检测SARS-CoV-2的mNGS引物组及试剂盒

权利要求书

1.检测SARS-CoV-2的mNGS引物组，其特征在于，所述引物组由172条引物序列组成，其碱基序列为：

SARS-CoV-2 RT-1 CATCACCAAAAGT

SARS-CoV-2 RT-2 TCGAGCATCCGAA

SARS-CoV-2 RT-3 CAAACCTTTCACA

SARS-CoV-2 RT-4 CCACAATGATCAC

SARS-CoV-2 RT-5 TCCCTCTAAGAAG

SARS-CoV-2 RT-6 CAGATTGATGTGA

SARS-CoV-2 RT-7 TAATTCAGGTTGC

SARS-CoV-2 RT-8 ATCACGGTCAAAT

SARS-CoV-2 RT-9 CACACACACTTAG

SARS-CoV-2 RT-10 AAATCCCACTTAA

SARS-CoV-2 RT-11 CATTCTCCTAAGA

SARS-CoV-2 RT-12 AGTGCTATTGAAA

SARS-CoV-2 RT-13 TAAGTCTACCTTT

SARS-CoV-2 RT-14 CTTGATTGTTCTT

SARS-CoV-2 RT-15 CACAGCCTCCAAA

SARS-CoV-2 RT-16 AAGGTTCTTTTAA

SARS-CoV-2 RT-17 TATAGTCTGGTAT

SARS-CoV-2 RT-18 ACAGCGTCCTAGA

SARS-CoV-2 RT-19 AGCCTTATTTAAG

SARS-CoV-2 RT-20 ATACTCTGCAAGA

SARS-CoV-2 RT-21 CATTAGTAACAAA

SARS-CoV-2 RT-22 TCCATTTGACTCC

SARS-CoV-2 RT-23 ATTTTGAAGGTCA

SARS-CoV-2 RT-24 TTTAAGTATAATG

SARS-CoV-2 RT-25 ACTATGGCAATCA

SARS-CoV-2 RT-26 CTACATGACTGTA

SARS-CoV-2 RT-27 TTGTTGTTGTTGG

SARS-CoV-2 RT-28 AAATAAGTAGTAT

SARS-CoV-2 RT-29 ATTTAAAACACTT

SARS-CoV-2 RT-30 CCACTCTGCAACT

SARS-CoV-2 RT-31 ATACTGTGAAATT

SARS-CoV-2 RT-32 TAACCATTAACTT

SARS-CoV-2 RT-33 TCTGGTTCTAGTG

SARS-CoV-2 RT-34 TTGAAAATCTTCA

SARS-CoV-2 RT-35 TAGTTAGAAGTAA

SARS-CoV-2 RT-36 CCATTAGCACACA

SARS-CoV-2 RT-37 TACCATCTGTTTT

SARS-CoV-2 RT-38 AAGCTTGGCGTAC

SARS-CoV-2 RT-39 ATCCAATTTATTC

SARS-CoV-2 RT-40 AGACGTAATCAAA

SARS-CoV-2 RT-41 GCTTCTGATCTTT

SARS-CoV-2 RT-42 CCGTGTACCAAGC

SARS-CoV-2 RT-43 AACACCTAGCTCT

SARS-CoV-2 RT-44 CCGTCTTGTTGAC

SARS-CoV-2 RT-45 ATACAACACAGTC

SARS-CoV-2 RT-46 AACATGCCAAACA

SARS-CoV-2 RT-47 CTCTACCATCAAA

SARS-CoV-2 RT-48 TTTGTAATGCAGC

SARS-CoV-2 RT-49 CCCAACAGCCTGT

SARS-CoV-2 RT-50 CTGGTAGAATTTC

SARS-CoV-2 RT-51 GGGTATAAGCCAG

SARS-CoV-2 RT-52 TTTAACATGTTGT

SARS-CoV-2 RT-53 AAATCTACCAATG

SARS-CoV-2 RT-54 TTAATTGGAACGC

SARS-CoV-2 RT-55 TAGGTAATTACTA

SARS-CoV-2 RT-56 TCATCAACTACAT

SARS-CoV-2 RT-57 TGTGACTTCCATG

SARS-CoV-2 RT-58 CCAAGTACCATAC

SARS-CoV-2 RT-59 GTCTACAGACAGC

SARS-CoV-2 RT-60 AAGTGGTATGAGG

SARS-CoV-2 RT-61 AACACTATCAACG

SARS-CoV-2 RT-62 GTACTCACTAGCA

SARS-CoV-2 RT-63 CAAGAATCTAAAT

SARS-CoV-2 RT-64 AGACTACACCATG

SARS-CoV-2 RT-65 TCTAAAACAACAC

SARS-CoV-2 RT-66 TTAGAAGAAGAAG

SARS-CoV-2 RT-67 TAATATCTCTGCT

SARS-CoV-2 RT-68 AGTTACTGAAGTC

SARS-CoV-2 RT-69 TAACATTGGAAAA

SARS-CoV-2 RT-70 TCACCAATCAAAG

SARS-CoV-2 RT-71 TTGTGGGTATTTC

SARS-CoV-2 RT-72 ACACTACCACATG

SARS-CoV-2 RT-73 AGGTTAAAGTCAT

SARS-CoV-2 RT-74 CACATGTCAACTT

SARS-CoV-2 RT-75 TGGTTTTGATGGA

SARS-CoV-2 RT-76 GGTAATAAACACC

SARS-CoV-2 RT-77 AAACAATTCTACA

SARS-CoV-2 RT-78 TTAAACACCGTGT

SARS-CoV-2 RT-79 ATTTCACAAGCAC

SARS-CoV-2 RT-80 ATGTTGTCTACTG

SARS-CoV-2 RT-81 GGGTTACGTGTAA

SARS-CoV-2 RT-82 GTGGTTATATAAT

SARS-CoV-2 RT-83 TGTGGCATCTCCT

SARS-CoV-2 RT-84 AGCTTTAACAACC

SARS-CoV-2 RT-85 GTAAATTGCAACT

SARS-CoV-2 RT-86 CATTAGTAAAGCA

SARS-CoV-2 RT-87 TAAAGAAGTCATG

SARS-CoV-2 RT-88 ACACTAATGCCTG

SARS-CoV-2 RT-89 CAACACGATGCAC

SARS-CoV-2 RT-90 GCACGATTGCAGC

SARS-CoV-2 RT-91 AGTTTGTGTAGTA

SARS-CoV-2 RT-92 AGGCAGAAACTTT

SARS-CoV-2 RT-93 AATGTATAGGGTC

SARS-CoV-2 RT-94 GTATCGTTGCAGT

SARS-CoV-2 RT-95 ATTATGATTAGAC

SARS-CoV-2 RT-96 GTGCAGGTATAAT

SARS-CoV-2 RT-97 CATCACCCAACTA

SARS-CoV-2 RT-98 GCAAAACAAGTAT

SARS-CoV-2 RT-99 TTCCAGAGTTGTT

SARS-CoV-2 RT-100 TGTCAAATCTACA

SARS-CoV-2 RT-101 TATTTTAATACCC

SARS-CoV-2 RT-102 CCCTGTGAATTCA

SARS-CoV-2 RT-103 CTCTGTGAAATAA

SARS-CoV-2 RT-104 TAAAGAACCTAGG

SARS-CoV-2 RT-105 TCCCATGAAAGAT

SARS-CoV-2 RT-106 AATTGTTTGGAGA

SARS-CoV-2 RT-107 TTCGTACTCATCA

SARS-CoV-2 RT-108 ATCATAATAAAGT

SARS-CoV-2 RT-109 CTTCCTTAAAGAA

SARS-CoV-2 RT-110 TGTACTCTATAAG

SARS-CoV-2 RT-111 AAGCAAAGTGTTA

SARS-CoV-2 RT-112 AACCGTCTACAAC

SARS-CoV-2 RT-113 TTTGATAGATTCC

SARS-CoV-2 RT-114 TGGGGATAGCACT

SARS-CoV-2 RT-115 ACCTTCCCAGGTA

SARS-CoV-2 RT-116 TTAAAGTTACAAA

SARS-CoV-2 RT-117 TTTTGTGGCACTG

SARS-CoV-2 RT-118 GGTGATTTACAAC

SARS-CoV-2 RT-119 GATGAGGATGAAG

SARS-CoV-2 RT-120 ATGCGTGAGCTTA

SARS-CoV-2 RT-121 ACGAGAAAACACA

SARS-CoV-2 RT-122 TGTCCGTGATCCA

SARS-CoV-2 RT-123 AGGCATTCAGTAC

SARS-CoV-2 RT-124 ATGCAGACCACAC

SARS-CoV-2 RT-125 GCGCTAACATAGG

SARS-CoV-2 RT-126 GAGTGTTCAGACA

SARS-CoV-2 RT-127 GCAAAGAAATTTG

SARS-CoV-2 RT-128 TAGGCTTGATGTG

SARS-CoV-2 RT-129 CAAACCACGCGAA

SARS-CoV-2RT-130 GGAGAGCCTTGTC

SARS-CoV-2RT-131 AGCTAGAAAATCA

SARS-CoV-2RT-132 TTGTTAACAACTA

SARS-CoV-2RT-133 GAACGCTTTCTTA

SARS-CoV-2RT-134 ACACAGAATGTTC

SARS-CoV-2RT-135 GCCTTTCTGCTAT

SARS-CoV-2RT-136 TAATAGCGTACTT

SARS-CoV-2RT-137 GCAGAAGGGAGCA

SARS-CoV-2RT-138 ACGAATTCGTGGT

SARS-CoV-2RT-139 GATCTCAGCTGGC

SARS-CoV-2RT-140 TTACATCATAAAC

SARS-CoV-2RT-141 ATCCCAAGATTTA

SARS-CoV-2RT-142 CATACATCACCAG

SARS-CoV-2RT-143 AATCAGCGAAATG

SARS-CoV-2RT-144 CTTATTACAGAAG

SARS-CoV-2RT-145 TTACATAGAAGTT

SARS-CoV-2RT-146 AAGAGGTTTGATA

SARS-CoV-2RT-147 GCAAATTGATAGG

SARS-CoV-2RT-148 AATGTCGCAAAAT

SARS-CoV-2RT-149 TGTGACATACCCA

SARS-CoV-2RT-150 ATTGAACGGTATA

SARS-CoV-2RT-151 AACAAATTTGCAC

SARS-CoV-2RT-152 TAAGCGCAACATT

SARS-CoV-2RT-153 TGGTGGCAGTTTG

SARS-CoV-2RT-154 TCAATTGAGTACA

SARS-CoV-2RT-155 GTAGTTGTCTCAA

SARS-CoV-2RT-156 CCTACAAAGCAAC

SARS-CoV-2RT-157 AACTGCTCCTGCC

SARS-CoV-2RT-158 CAAATTGGATGAC

SARS-CoV-2RT-159 GATTTGGCTACTA

SARS-CoV-2RT-160 TACAAATGTTAAG

SARS-CoV-2RT-161 TTACAATCATATG

SARS-CoV-2RT-162 ATGTACGACCCTA

SARS-CoV-2RT-163 CTAGTGCGAATAA

SARS-CoV-2RT-164 TGATTCAACTGAA

SARS-CoV-2RT-165 TTGTTAGATTTCC

SARS-CoV-2RT-166 CTTCTAAGAAGCC

SARS-CoV-2RT-167 CACTTGCAGATGC

SARS-CoV-2RT-168 GTAACCCTAAAGC

SARS-CoV-2RT-169 TTGGCGTTGCACT

SARS-CoV-2RT-170 TATTACCGTTGAA

SARS-CoV-2RT-171 GTGTGAATATCAC

SARS-CoV-2RT-172 AACAATTGTTGAG。