[发明专利]基于MassArray核酸质谱的脑胶质瘤染色体lp/19q检测方法及应用

权利要求书

1.一种用于检测脑胶质瘤染色体lp/19q杂合性缺失相关SNP位点的引物组，其特征在于，所述SNP位点包括：rs933032、rs4970520、rs1329113、rs586057、rs624971、rs2038366、rs6426368、rs1457093、rs7512426、rs67421541、rs36624、rs79887225、rs797257、rs2031958、rs16866144、rs10407280、rs12611404、rs10875362、rs212961、rs437229、rs2473287、rs1674139、rs859104、rs11666952、rs11247639、rs4908744、rs9426469、rs4803502、rs11586551、rs3766345、rs7507651、rs2385126、rs12039701、rs621996、rs2641114、rs2459143、rs891167、rs12032257、rs11879622、rs2914658、rs7359978、rs33824、rs4927030、rs58568071、rs10776736、rs260502、rs2015、rs3972312、rs4847150、rs1199668；所述引物组分为如下3组：

第一组包括检测rs933032、rs4970520、rs1329113、rs586057、rs624971、rs2038366、rs6426368、rs1457093、rs7512426、rs67421541、rs36624和rs79887225的引物组；

第二组包括检测rs797257、rs2031958、rs16866144、rs10407280、rs12611404、rs10875362、rs212961、rs437229、rs2473287、rs1674139、rs859104、rs11666952、rs11247639、rs4908744、rs9426469和rs4803502的引物组；

第三组包括检测rs11586551、rs3766345、rs7507651、rs2385126、rs12039701、rs621996、rs2641114、rs2459143、rs891167、rs12032257、rs11879622、rs2914658、rs7359978、rs33824、rs4927030、rs58568071、rs10776736、rs260502、rs2015、rs3972312、rs4847150和rs1199668的引物组；

所述引物组序列如SEQ ID NO.1-100所示；

所述引物组进一步包括SEQ ID NO.101-150所示的延伸引物。

2.一种用于检测脑胶质瘤染色体lp/19q杂合性缺失的产品，其特征在于，所述产品包括权利要求1所述的引物组。

3.一种用于检测脑胶质瘤染色体lp/19q杂合性缺失的试剂盒，其特征在于，所述试剂盒包括权利要求1所述的引物组。

4.权利要求1所述的引物组在制备脑胶质瘤检测产品中的应用。