[发明专利]转氨酶突变体及其应用

权利要求书

1.一种转氨酶突变体，其特征在于，所述转氨酶突变体是由SEQ ID NO：1所示的氨基酸序列发生突变得到，所述突变为T291A或为如下突变位点组合之一：T291A+S290L、T291A+S290V、T291A+S290T、T291A+G292A、T291A+G292L、T291A+G292I、T291A+G292D、T291A+G292A+Y75T、T291A+G292A+G77E、T291A+G292A+M130I、T291A+G292A+M130L、T291A+G292A+Y144S、T291A+G292A+Y144G、T291A+G292A+Y144I、T291A+G292A+Y144V、T291A+G292A+Y144P、T291A+G292A+Y144L、T291A+G292A+Y144H、T291A+G292A+Q163S、T291A+G292A+Q163T、T291A+G292A+Q163L、T291A+G292A+Q163N、T291A+G292A+Q163V、T291A+G292A+W164F、T291A+G292A+W164R、T291A+G292A+W164L、T291A+G292A+W164T、T291A+G292A+I165A、T291A+G292A+I165V、T291A+G292A+I165L、T291A+G292A+W200G、T291A+G292A+T70C、T291A+G292A+T70C+W164F、T291A+G292A+T70C+F233Y、T291A+G292A+T70C+I165V、T291A+G292A+T70C+Y144M+G145N、T291A+G292A+T70C+Y144M+G145S、T291A+G292A+T70C+G145L、T291A+G292A+T70C+Y69L、T291A+G292A+T70C+T142G、T291A+G292A+T70C+I160G、T291A+G292A+T70C+I160T、T291A+G292A+T70C+I160S、T291A+G292A+T70C+I160V、T291A+G292A+T70C+I160C、T291A+G292A+T70C+S204L、T291A+G292A+T70C+S204M、T291A+G292A+T70C+V244G、T291A+G292A+T70C+V244R、T291A+G292A+T70C+V244Y、T291A+G292A+T70C+V244S、T291A+G292A+T70C+V244K、T291A+G292A+T70C+V244M、T291A+G292A+T70C+I160G+H151A、T291A+G292A+T70C+I160G+H151D、T291A+G292A+T70C+I160G+H151Y、T291A+G292A+T70C+I160G+H151Q、T291A+G292A+T70C+I160G+S204L、T291A+G292A+T70C+I160G+V244S、T291A+G292A+T70C+I160G+S204L+V244S、T291A+G292A+T70C+I160G+T134I、T291A+G292A+T70C+I160G+A242S、T291A+G292A+T70C+I160G+A242Q、T291A+G292A+T70C+I160G+T270G、T291A+G292A+T70C+I160G+T270H、T291A+G292A+T70C+I160G+S278L、T291A+G292A+T70C+I160G+S278R、T291A+G292A+T70C+I160G+S278K、T291A+G292A+T70C+I160G+E282C、T291A+G292A+T70C+I160G+E282R、T291A+G292A+T70C+I160G+E282K、T291A+G292A+T70C+I160G+M326Q、T291A+G292A+T70C+I160G+V17S、T291A+G292A+T70C+I160G+A56S、T291A+G292A+T70C+I160G+F64L、T291A+G292A+T70C+I160G+P167A、T291A+G292A+T70C+I160G+P167K、T291A+G292A+T70C+I160G+R216E、T291A+G292A+T70C+I160G+R216K、T291A+G292A+T70C+I160G+S204L+V244S+M326Q、T291A+G292A+T70C+I160G+S204L+V244S+T134I、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y158I、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y158V、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+R216N、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+R216K、T291A+G292A+L232F、T291A+G292A+F233W、T291A+G292A+F233Y、T291A+G292A+T70G、T291A+G292A+T70M、T291A+G292A+T70A、T291A+G292A+T70S、T291A+G292A+W164Y、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+G77V、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+W200A、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144L、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144L+G77T、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144L+G77L、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+A292G、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+A292G+G77T、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+W200A+A56P、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+W200A+A56P+N132A、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+W200A+A56P+G77S、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+W200A+A56P+G77T、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+W200A+A56P+Y144F、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144L+G77T-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+A292G+G77T-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144L+G77T+L144W-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144L+G77T+L144P-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144L+G77T+N132R-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144L+G77T+I165L-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144L+G77T+I165H-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165H-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+I78V-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A291I-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+I78H-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+T142V-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+G145H-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+G145D-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+A148T-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+A148Q-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+R152K-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+R152T-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+F233Y-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69M+G145H-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69M+G145D-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69M+A148T-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69M+A148Q-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69M+R152K-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69M-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69C-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69G-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69H-12aa、T291A+G292A+T70G+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69H -12aa、T291A+G292A+T70S+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69H -12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69H+G140C-12aa、T291A+G292A+T70C+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69H+S141G-12aa、T291A+G292A+T70A+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69H+G140C -12aa、T291A+G292A+T70G+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69H +Y139S-12aa、T291A+G292A+T70G+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69H +G160T-12aa、T291A+G292A+T70G+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69H +G160T+S292A-12aa、T291A+G292A+T70G+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69H +G160T+A291T-12aa、T291A+G292A+T70G+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69H +G160T+I134V-12aa、T291A+G292A+T70G+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69H+ G160T+T106S-12aa、或T291A+G292A+T70G+I160G+S204L+V244S+T134I+M326Q+Y144W+G77T+I165L+A292S+S278R+Y69H +G160T+Q171T-12aa。