[发明专利]编码构巢曲霉中异青霉素N合成酶的重组DNA表达载体和DNA化合物

权利要求书

1、一种在重组体宿主细胞中表达曲霉属异青霉素N合成酶活性的方法，该方法包括：

(1)用重组DNA表达载体转化宿主细胞，该载体包括：

(a)在宿主细胞中起功能作用的启动子和转译活性序列；和

(b)编码曲霉属异青霉素N合成酶活性的DNA序列，所述序列的排例适用于从启动子和转译活性序列表达；和

(2)在允许曲霉属异青霉素N合成霉活性表达的条件下培养在步骤(1)中经过转化的宿主细胞。

2、权利要求1所述方法，其中编码曲霉属异青霉素N合成酶活性的重组DNA序列包括氨基酸残基序列：

H₂N-MET GLY SER VAL SER LYS ALA ASN VAL PRO LYS ILE ASP VAL SER PRO LEU PHE GLY ASP ASP GLN ALA ALA LYS MET ARG VAL ALA GLN GLN ILE ASP ALA ALA SER ARG ASP THR GLY PHE PHE TYR ALA VAL ASN HIS GLY ILE ASN VAL GLN ARG LEU SER GLN LYS THR LYS GLU PHE HIS MET SER ILE THR PRO GLU GLU LYS TRP ASP LEU ALA ILE ARG ALA TYR ASN LYS GLU HIS GLN ASP GLN VAL ARG ALA GLY TYR TYR LEU SER ILE PRO GLY LYS LYS ALA VAL GLU SER PHE CYS TYR LEU ASN PRO ASN PHE THR PRO ASP HIS PRO ARG ILE GLN ALA LYS THR PRO THR HIS GLU VAL ASN VAL TRP PRO ASP GLU THR LYS HIS PRO GLY PHE GLN ASP PHE ALA GLU GLN TYR TYR TRP ASP VAL PHE GLY LEU SER SER ALA LEU LEU LYS GLY TYR ALA LEU ALA LEU GLY LYS GLU GLU ASN PHE PHE ALA ARG HIS PHE LYS PRO ASP ASP THR LEU ALA SER VAL VAL LEU ILE ARG TYR PRO TYR LEU ASP PRO TYR PRO GLU ALA ALA ILE LYS THR ALA ALA ASP GLY THR LYS LEU SER PHE GLU TRP HIS GLU ASP VAL SER LEU ILE THR VAL LEU TYR GLN SER ASN VAL GLN ASN LEU GLN VAL GLU THR ALA ALA GLY TYR GLN ASP ILE GLU ALA ASP ASP THR GLY TYR LEU ILE ASN CYS GLY SER TYR MET ALA HIS LEU THR ASN ASN TYR TYR LYS ALA PRO ILE HIS ARG VAL LYS TRP VAL ASN ALA GLU ARG GLN SER LEU PRO PHE PHE VAL ASN LEU GLY TYR ASP SER VAL ILE ASP PRO PHE ASP PRO ARG GLU PRO ASN GLY LYS SER ASP ARG GLU PRO LEU SER TYR GLY ASP TYR LEU GLN ASN GLY LEU VAL SER LEU ILE ASN LYS ASN GLY GLN THR-COOH

其中ALA是丙氨酸残基，ARG是精氨酸残基，ASN是天门冬酰胺残基，ASP是天冬氨酸残基，-COOH是羧基末端，CYS是半胱氨酸残基，GLN是谷氨酰胺残基，GLU是谷氨酸残基，GLY是甘氨酸残基，HIS是组氨酸残基，H₂N-是氨基末端，ILE是异亮氨酸残基，LEU是亮氨酸残基，LYS是赖氨酸残基，MET是甲硫氨酸残基，PHE是苯丙氨酸残基，PRO是脯氨酸残基，PHE是苯丙氨酸残基，PRO是脯氨酸残基，SER是丝氨酸残基，THR是苏氨酸残基，TRP是色氨酸残基，TYR是酪氨酸残基，VAL是缬氨酸残基。