[发明专利]检测药物活性成分对相关基因表达调控的生物芯片

摘要

本发明公开了一种检测药物活性成分对相关基因表达调控的生物芯片，在玻片或硅片或高分子材料上固定筛选的基因DNA探针，检测基因的DNA探针有PCNA、CyclinA1、CyclinB1、CyclinC、CyclinD1、CyclinD1、CyclinE1、CyclinG1、Cdk4、Cdk2、Cdc2、p16、p21、p53、E2F1、Chk1、Chk2、ATM、DNA－PK、DNA－Topo I、hTERT、Bcl－2、Trail、Mcl－1、Bax、Bak1、Brca1、Caspase3、Erk－3、Jnk、p38、Cdc25A、Fgd1、Rac和VEGF的探针，本发明可以快速地检测药物活性成分对相关基因表达调控，同一芯片可以同时检测，并具有实时、灵敏、准确和高效等特点。

主权项

1.一种检测药物活性成分对相关基因表达调控的生物芯片，其特征是在玻片或硅片或高分子材料上固定筛选的基因DNA探针，所述探针如下：PCNA tgtcgataaa gaggaggaag ctgttaccat agagatgaat gaaccCyclin A1 ggaagctgaa ataaggcaca gacccaaagc acactacatg aagaaCyclin B1 aaatgaagat tctaagagct ttaaactttg gtctgggtcg gcctcCyclin C gttattgcca ctgctacggt atatttcaag agattctatg ccaggCyclin D1 tgtgccacag atgtgaagtt catttccaat ccgccctcca tgCyclin E1 cctgctccct gatccccaca cctgacaaag aagatgatgaCyclin G1 ctgcacacga taatggcctc agaatgactg caagactaag ggactCdk4 cacagttcgt gaggtggctt tactgaggcg actggaggct ttCdk2 cctgtggtac cgagctcctg aaatcctcct gggctgcaaa taCdc2 gattctatcc ctcctggtca gtacatggat tcttcacttg ttp16 catagatgcc gcggaaggtc cctcagacat ccccgattgp21 atgtggacct gtcactgtct tgtacccttg tgcctcgctc agp53 tgaagaccca ggtccagatg aagctcccag aatgccagagE2F1 caagccagga agggaaggag tctgtgtggt gtgtatgtgc atCHK1 tgaagaagca gtcgcagtga agattgtaga tatgaagcgt gccgtCHK2 ctctaccagc acgatgccaa actccagcca gtcctctcac tcATM gccgtcaact agaacatgat agagctacag aacgaaagaa agaagPCNA cgataaagag gaggaagctg ttaccataga gatgaatgaa ccCyclin A1 ctgaaataag gcacagaccc aaagcacact acatgaagaa gcagcCyclin B1 gttggactat gacatggtgc actttcctcc ttctcaaatt gCyclin C atggaggata gtgaatgata cctacagaac ggatctttgc cCyclin D1 acaacttcct gtcctactac cgcctcacac gcttcctctc caCyclin E1 ccttgaattt ccttatggta tacttgctgc ttcggccttgCyclin G1 tattggagaa ggtgtgttgg aaagtcaaag ctactactgc cCdk4 cccgtggttg ttacactctg gtaccgagct cccgaagttc ttCdk2 ccctggatga agatggacgg agcttgttat cgcaaatgct gCdc2 agtatataca catgaggtag taacactctg gtacagatct ccp16 agaggcagta accatgcccg catagatgcc gcggaaggtp21 aaacggcggc agaccagcat gacagatttc taccactcca aacp53 gtggtaatct actgggacgg aacagctttg aggtgcatgtE2F1 caagccagga agggaaggag tctgtgtggt gtgtatgtgc atCHK1 tgatgtttgg tcctgtggaa tagtacttac tgcaatgctc gctggCHK2 tgccaatctt gaatgtgtga atgacaacta ctggtttggg aATM tgctgttacc aaaggatgct gttctcagac tgacggatta aattcDNA-PK gtgaccaaca gctgcgcaag gaggaagaga atgcatcagt taTopoI gaagaaagaa gaggaacaga agtggaaatg gtgggaagaa ghTERT tcaaccgcgg cttcaaggct gggaggaaca tgcgtcgcaa aBcl-2 acgctgggag aacagggtac gataaccggg agatagtgat gaagtTrail cacaaacaca aagataaaca caaagatcga gaacaccggc atMCL-1 aggaggagga ggacgagttg taccggcagt cgctggagat tBAX gggacgaact ggacagtaac atggagctgc agaggatgat tgBAK1 accgacgcta tgactcagag ttccagacca tgttgcagca ccBRCA1 ccttgcagga aaccagtctc agtgtccaac tctctaacct tgCaspase 3 tctcggtctg gtacagatgt cgatgcagca aacctcaggg aaERK-3 ccaattaaca gacgatgtgg gctctcttac ggaactgaac agJNK ttcatcacca acacggacgt cttcatcgcc atggagctcap38 agcctcctcc ctgcgcaact tctatgactt ctacctggtgCdc25A actttgtctg atgaggatga tggcttcgtg gaccttctcFGD1 ctgattgaga agtttgaaag agagcctgtg attgtcgcctRac taaagacacg atcgagaaac tgaaggagaa gaagctgactVEGF aggcgaggca gcttgagtta aacgaacgta cttgcagatg tgacaβ-actin gacctggctg gccgggacct gactgactac ctcatgaaga tcDNA-PK acttcagatt atagaacggt atccagagga gactttgagc cTopoI ataaacacaa agatcgagaa caccggcaca aagaacacaa ghTERT catctctgac acggcctccc tctgctactc catcctgaaa gBcl-2 tggatgactg agtacctgaa ccggcacctg cacacctggTrail acaaagataa acacaaagat cgagaacacc ggcataagga gcMCL-1 tgaagaccat aaaccaagaa agctgcatcg aaccattagc aBAX cgagtggcag ctgacatgtt ttctgacggc aacttcaact ggBAK1 aatgcctatg agtacttcac caagattgcc accagcctgt ttBRCA1 cttattgcag tgtgggagat caagaattgt tacaaatcac ccCaspase 3 gtgccatgct gaaacagtat gccgacaagc ttgaatttat gcERK-3 gtttacattg ttcaggagta catggagaca gacttggcta atJNK ggcagaaatc aacgacctgg agaacttggg cgagatgggcp38 cggacctgct ggagaagatg ctggagctag acgtggacaaCdc25A tagctaccgg cccatgcacc acgaggactt taaagaagacFGD1 catcttctct aacatctgct ccatctattg cttccaccagRac catcaagtgt gtggtggtgg gagacggagc tgtaggtaaaVEGF gccgccacca caccatcacc atcgacagaa cagtccttaa tcβ-actin gagcgggaaa tcgtgcgtga cattaaggag aagctgtgct ac