[发明专利]日本血吸虫重组多表位抗原及其表达、纯化方法与应用

摘要

本发明公开了日本血吸虫重组多表位抗原BSjGCP-BSj23和BSjGCP-BSj23-BSj28的基因序列和表达、纯化方法及在制备日本血吸虫病免疫预防疫苗和诊断试剂中的应用。重组多表位核酸疫苗pCMV-BSjGCP-BSj23和pCMV-BSjGCP-BSj23-BSj28在昆明系小鼠中分别获得了14.76％、64.95％的减虫率。重组多表位抗原pGEX-BSjGCP-BSj23和pGEX-BSjGCP-BSj23-BSj28免疫BalB/c小鼠获得了15.7％、57.99％的减虫率，作为诊断抗原分别获得了91.0％、89.9％的敏感性和97.8％、93.4％的特异性。

主权项

1、一种日本血吸虫重组多表位抗原，其特征在于所述多表位表原为BSjGCP-BSj23，它由日本血吸虫23KD抗原表位和日本血吸虫抱雌沟蛋白抗原表位重组组成，具有如下核酸序列和相应的蛋白序列：BSjGCP-BSj23核酸序列：atgcgaattg gatatgaggg tctaccacgt gatcaatggc caaaagtgat tcattggaat 60ctacatgcac gtgacggtat tatctgggta ttagatggtc tattgaaatg tccggaaaaa 120ctttgcccat tacttgctga agatgttgat tattattcta gaatgactgg tgctctggaa 180aatccaaacg aggaaataac ggcaaccatg gataagatac aaacgtcatt ccattgttgt 240ggagtcaaag gtccagacga ttataaaggg aatgtgccag catcatgtaa agaagggcaa 300gaagtttatg ttcagggttg tctatctgtc tttagtgcat tcttgaaacg caac 354BSjGCP-BSj23蛋白序列：Gly Ser Met Arg Ile Gly Tyr Glu Gly Leu Pro Arg Asp Gln Trp Pro1 5 10 15Lys Val Ile His Trp Asn Leu His Ala Arg Asp Gly Ile Ile Trp Val20 25 30Leu Asp Gly Leu Leu Lys Cys Pro Glu Lys Leu Cys Pro Leu Leu Ala35 40 45Glu Asp Val Asp Tyr Tyr Ser Arg Met Thr Gly Ala Leu Glu Asn Pro50 55 60Asn Glu Glu Ile Thr Ala Thr Met Asp Lys Ile Gln Thr Ser Phe His65 70 75 80Cys Cys Gly Val Lys Gly Pro Asp Asp Tyr Lys Gly Asn Val Pro Ala85 90 95Ser Cys Lys Glu Gly Gln Glu Val Tyr Val Gln Gly Cys Leu Ser Val100 105 110Phe Ser Ala Phe Leu Lys Arg Asn Lys Leu115 120。