[发明专利]芸薹属核心SSR引物组合

摘要

本发明公开了芸薹属核心SSR引物组合，属于分子遗传技术领域。该组合包括芸薹属A和C基因组19条染色体的核心SSR引物190对，每条染色体上均匀分布有10对多态性较高、退火温度基本一致、扩增带型清楚的SSR引物。运用该核心SSR引物组合在不增加工作成本的前提下，可将分子标记的效率提高到3-5倍，特别适合芸薹属种质资源的鉴定、遗传多样性分析、缺体等异常染色体单株的鉴定和种子纯度鉴定等方面。具有操作简单，稳定性和重复性好等优点。

主权项

芸薹属核心SSR引物组合，包含19条染色体即A1‑A10和C1‑C9核心SSR引物，其特征在于，包括以下引物：A1  CB10369      ENA15  CB10097     NIAB096     Ra2E04      BRAS026  Na12C06      CNU397    Ol10D03       CB10597A2  Ol10F04     Ol09A03     NIAB105SR6293     CB10416      Na12A07     CB10093    Ni2C12B  Na14H11C  BRAS083   A3  SR6688 BRMS071    NIAB120 CNU482   CNU409      BRMS176    Ol10B08      SN1919  Ol13D02A       Na12C07      A4  FITO066      SN13034      sNRD71       ENA3    Ol13D02A   CNU254      BRAS003    CB10196      CB10335       CB10493      A5 sNRD03      BRMS034   FITO130     BRAS063   NIAB082   SR9222ENA10  CB10080      SR9477       CB10487      A6 CB10330  CB10143   BRAS014  BRMS227    BRMS221    CB10065      BRMS226    BRMS261    CNU400 EJU5A7 Ra2G08  SR0282R     FITO066      NIAB082     BRMS298    NIAB030     Ol12E03       Ra2A05 CB10211       CB10278      A8  CNU090      BRMS342    BRMS246    BRMS176    BRMS185    EJU4     KBRH143D22    Ra2E12 EJU3       CB10193      A9  EJU2     CB10347      BRMS324    FITO100      Ol12F02       CB10255      Ol10D08      CB10103      BRAS055       MR013A      A10 Ol10A02    NIAB015    BRMS186   SN8502  SN8474  NIAB103     ENA18  BRMS019    CB10109       CB10124C1  CB10587      CB10369      CB10277      FITO094      NIAB091     CB10206      Na10H06     Ol10F11       Ni4B10 Na10H03C2 Ol11H09     CB10316     SR6293  CB10026      CB10530      FITO081      Ol09A06      Ol09A03      Ol12B03       Na12C03C3 Ra2E11  BN12A  CB10036      BRAS120    MR049  Na10C01      BRAS065    MR061B      MR049B       CB10003      C4 SN0464  SS2277  BRMS166    Na10F06      Ol12D02      SN11516      CB10288      CB10493      BRAS072       Ol11E03AC5  CB10124      sORH13       BRMS030    BRMS049    CB10623      Ra2F11  MR129  MR097  CB10229       CB10487      C6  CB10502      CB10234      CB10010      BRMS015    SR12387      CB10526      FITO146      CB10544       SS2486  CB10211      C7 SN0706  sNRH63      Ol10B01     Ra3C04  BRMS050    FITO088      BRMS040    CB10299      CB10534       CB10431C8 CB10139     FITO040     SN11670     SR5795Ol12G04      CB10106      FITO424      SN12352      CB10602       BRAS031    C9  BRAS002    FITO088      SR12384I     CB10288      BRAS050    Ol11H05      FITO016      sNRG42       Ra2F11  Ol11H06。