[发明专利]一种稳定、抗干扰能力强的血清尿素检测方法及试剂在审
| 申请号： | 201510211501.9 | 申请日： | 2015-04-29 |
| 公开（公告）号： | CN104749124A | 公开（公告）日： | 2015-07-01 |
| 发明（设计）人： | 甘宜梧;李志明;谭柏清 | 申请（专利权）人： | 山东博科生物产业有限公司 |
| 主分类号： | G01N21/31 | 分类号： | G01N21/31 |
| 代理公司： | 暂无信息 | 代理人： | 暂无信息 |
| 地址： | 250200 山东省济南市*** | 国省代码： | 山东;37 |
| 权利要求书： | 查看更多 | 说明书： | 查看更多 |
| 摘要： | 本发明提供一种抗干扰能力强、稳定性好的血清尿素检测方法及试剂。采用酶偶联速率法，可以有效检测血清尿素的含量，具有抗干扰能力强、稳定性好等优点。该产品包括试剂R1和试剂R2，采用新的缓冲体系和稳定剂，显著改善了试剂的稳定性。同时采用新型表面活性剂烷基糖苷，不仅显著改善测定的性能，而且增强了试剂的稳定性和抗干扰能力，完全可以满足临床需要。 | ||
| 搜索关键词： | 一种 稳定 抗干扰 能力强 血清 尿素 检测 方法 试剂 | ||
【主权项】：
一种稳定、抗干扰能力强的血清尿素检测方法及试剂，其特征在于包括试剂R1和试剂R2，所述试剂R1和试剂R2的组成如下：试剂R1：PIPES（哌嗪‑1,4‑二乙磺酸）缓冲液（pH=7.6）···································100mmo1/Lα‑酮戊二酸·································································································20mmo1/L谷氨酸脱氢酶····························································································800U/LADP···········································································································2mmo1/LBSA············································································································1g/L海藻糖·········································································································2g/L蔗糖············································································································20g/LAPG(烷基糖苷)···························································································2g/L叠氮钠（NaN3）···························································································0.5g/L试剂R2：PIPES（哌嗪‑1,4‑二乙磺酸）缓冲液（pH=9.4）···································100mmo1/L脲酶···········································································································10KU/LNADH········································································································1.2mmo1/LBSA············································································································1g/L海藻糖·········································································································2g/L蔗糖············································································································20g/LAPG(烷基糖苷)··························································································2g/L叠氮钠（NaN3）···························································································0.5g/L 。
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