[发明专利]生物合成灭活素的方法

摘要

本发明涉及产生一种新的人类蛋白质-灭活素。通过重组DNA技术，确定该基团的序列，从重组宿主大量表达物纯化生物学上具有活性的灭活素。本发明还涉及纯化有生物活性的天然灭活素，以及由灭活素及其氨基酸顺序所衍生的多肽的方法。

主权项

1.一种生物合成灭活素的方法，该方法包括：a)用一种重组DNA分子转化一种宿主细胞，所述重组DNA分子包含编码具有以下氨基酸顺序的蛋白质的cDNA和载体DNA分子，ATGGAGGATCTTTGTGTGGCAAACACACTCTTTGCCCTCAATTTAMetGluAspLeuCysValAlaAsnThrLeuPheAlaLeuAsnLeuTTCAAGCATCTGGCAAAAGCAAGCCCCACCCAGAACCTCTTCCTCPheLysHisLeuAlaLysAlaSerProThrGlnAsnLeuPheLeuTCCCCATGGAGCATCTCGTCCACCATGGCCATGGTCTACATGGGCSerProTrpSerIleSerSerThrMetAlaMetValTyrMetGlyTCCAGGGGCAGCACCGAAGACCAGATGGCCAAGGTGCTTCAGTTTSerArgGlySerThrGluAspGlnMetAlaLysValLeuGlnPheAATGAAGTGGGAGCCAATGCAGTTACCCCCATGACTCCAGAGAACAsnGluValGlyAlaAsnAlaValThrProMetThrProGluAsnTTTACCAGCTGTGGGTTCATGCAGCAGATCCAGAAGGGTAGTTATPheThrSerCysGlyPheMetGlnGlnIleGlnLysGlySerTyrCCTGATGCGATTTTGCAGGCACAAGCTGCAGATAAAATCCATTCAProAspAlaIleLeuGlnAlaGlnAlaAlaAspLysIleHisSerTCCTTCCGCTCTCTCAGCTCTGCAATCAATGCATCCACAGGGAATSerPheArgSerLeuSerSerAlaIleAsnAlaSerThrGlyAsnTATTTACTGGAAAGTGTCAATAAGCTGTTTGGTGAGAAGTCTGCGTyrLeuLeuGluSerValAsnLysLeuPheGlyGluLysSerAlaAGCTTCCGGGAAGAATATATTCGACTCTGTCAGAAATATTACTCCSerPheArgGluGluTyrIleArgLeuCysGlnLysTyrTyrSerTCAGAACCCCAGGCAGTAGACTTCCTAGAATGTGCAGAAGAAGCTSerGluProGlnAlaValAspPheLeuGluCysAlaGluGluAlaAGAAAAAAGATTAATTCCTGGGTCAAGACTCAAACCAAAGGCAAAArgLysLysIleAsnSerTrpValLysThrGlnThrLysGlyLysATCCCAAACTTGTTACCTGAAGGTTCTGTAGATGGGGATACCAGGIleProAsnLeuLeuProGluGlySerValAspGlyAspThrArgATGGTCCTGGTGAATGCTGTCTACTTCAAAGGAAAGTGGAAAACTMetValLeuValAsnAlaValTyrPheLysGlyLysTrpLysThrCCATTTGAGAAGAAACTAAATGGGCTTTATCCTTTCCGTGTAAACProPheGluLysLysLeuAsnGlyLeuTyrProPheArgValAsnTCGGCTCAGCGCACACCTGTACAGATGATGTACTTGCGTGAAAAGSerAlaGlnArgThrProValGlnMetMetTyrLeuArgGluLysCTAAACATTGGATACATAGAAGACCTAAAGGCTCAGATTCTAGAALeuAsnIleGlyTyrIleGluAspLeuLysAlaGlnIleLeuGluCTCCCATATGCTGGAGATGTTAGCATGTTCTTGTTGCTTCCAGATLeuProTyrAlaGlyAspValSerMetPheLeuLeuLeuProAspGAAATTGCCGATGTGTCCACTGGCTTGGAGCTGCTGGAAAGTGAAGluIleAlaAspValSerThrGlyLeuGluLeuLeuGluSerGluATAACCTATGACAAACTCAACAAGTGGACCAGCAAAGACAAAATGIleThrTyrAspLysLeuAsnLysTrpThrSerLysAspLysMetGCTGAAGATGAAGTTGAGGTATACATACCCCAGTTCAAATTAGAAAlaGluAspGluValGluValTyrIleProGlnPheLysLeuGluGAGCATTATGAACTCAGATCCATTCTGAGAAGCATGGGCATGGAGGluHisTyrGluLeuArgSerIleLeuArgSerMetGlyMetGluGACGCCTTCAACAAGGGACGGGCCAATTTCTCAGGGATGTCGGAGAspAlaPheAsnLysGlyArgAlaAsnPheSerGlyMetSerGluAGGAATGACCTGTTTCTTTCTGAAGTGTTCCACCAAGCCATGGTGArgAsnAspLeuPheLeuSerGluValPheHisGlnAlaMetValGATGTGAATGAGGAGGGCACTGAAGCAGCCGCTGGCACAGGAGGTAspValAsnGluGluGlyThrGluAlaAlaAlaGlyThrGlyGlyGTTATGACAGGGAGAACTGGACATGGAGGCCCACAGTTTGTGGCAValMetThrGlyArgThrGlyHisGlyGlyProGlnPheValAlaGATCATCCTTTTCTTTTTCTTATTATGCATAAGATAACCAACTGCAspHisProPheLeuPheLeoIleMetHisLysIleThrAsnCysATTTTATTTTTCGGCAGATTTTCCTCACCCTAAIleLeuPhePheGlyArgPheSerSerPro***b)在适于灭活素表达的条件下培养转化的细胞，和c)回收所表达的灭活素。