[发明专利]KIT信号相关基因QPCR芯片

权利要求书

1.一种KIT信号相关基因QPCR芯片，其特征在于：所述芯片以Akt1、Bcl2、Cbl 、Ccnd3、Ccnd3-ps、Csf1、Crk、Ecm1、Fes、Fyn、Grb7、Hras1、Jak1、Kitl、Lyn、Map2k、Matk、Myb、Nr0b2、Ntrk1、Pdgfa、Pik3ca、Ppp1r13b、Prkcc、Raf1、Sh2b2、Shc1、Snai2、Soat1、Stat1、Zhx2基因为检测位点，以Actb为内参，以以下32个基因的上游引物和下游引物为基础检测特定基因的表达水平：

基因名称上游引物下游引物ActbF: AGATGACCCAGATCATGTTTGAGAR: AGATGACCCAGATCATGTTTGAGA     Akt1  F:TGAGGAGCGGGAAGAATGG  R:GGTGAGCCTGATCGGAAGTCBcl2  F: GAGGCTGGGATGCCTTTGT               R:CCAGGTATGCACCCAGAGTGACblF: GAGCGCCGGCGGTGGTTGC             R: GGAATGGAGCCGGGCGAGAAGGA       Ccnd3   F: TGCGTGCAAAAGGAGATCAA         R: CACACACCTCCAGCATCCAGTACcnd3-psF: CCTGTCTCTGCCCAGTGGTT   R: CCAAAAAGTCTGGGCATGCT                 CrkF:CCAACCCAGCGTCAACACT     R:GCTCACCGACCTCCAAAGC     Csf1F: GCAGCAGTTGATCGACAGTCA     R: CGCATGGTCTCATCTATTATGTCTTG  Ecm1F: TGCCGCAGCGACACAA                  R: CATTGCATCCTCCCACACAAGFesF: AGCAAGGATCCTGAAACAGTACAA    R: TGTGCAGACCCCGATGAGA                   Fyn       F:CCATACCCAGGCATGAACAAC      R:GTGGGCAGGGCATCCTATAG    Grb7F: CTGGATGCACAAACAGGCATA       R: TGCAGGAAGCCCTGGATCTHras1F: CAAGCTGGCGTGGAGGATR: CGCAATTTATGCTGCCGAATJak1F: CCGCATGAGGTTCTACTTTACCAR: TGTCGCCATACAGACTGTTCGTKitlF: GCACAGTGGCTGGTAACAGTTCR: GAACAGGTAAGGATGAGGTCTGTCALynF: CTACGTGGCCAAGGTCAACA   R:CTGTCGCTCTGCATCTTTCCTMap2kF: ACAGAGATGTGAAGCCCTCCAAR: CCCCGAAGTCACACAGCTTAAMatkF: GCAGTTTGCTCTTCATGTTGCTR: CAAGTCCTCAGAGACCAGGATGTMybF: CTGAACCCTGAACTCATCAAAGGR: GACCAACGCTTCGGACCATANr0b2F: GAGCTGGGTCCCAAGGAGTATR: GCACATCTGGGTTGAAGAGGATNtrk1F: TCTGGGAGATCTTCACCTATGGAR: CGATCGCCTCAGTGTTGGAPdgfaF: GTGCGACCTCCAACCTGAAR:GCTCATCTCACCTCACATCTGTCTPik3caF: CGAGATATATGATGCAGCCATTGR: AGATAAAGGTTGCCACGCAGTACPpp1r13bF: TGCTGGACTTTGGTGTCAATGR: GCAAGAGGCAGCACAATGCPrkccF: AGCAACTGGGCAAGTTTAAGGAR: GAAGAAGAGGCCTATGGCGATTRaf1F: AGCTTCCCTACGCCCACATR:ACCCACGGCCTACCATGASerpina1cF: CTGGACCAAGACACAGTTTTCGR: GCTTCTTCCATTTGCCTTTAAAGASh2b2F: TCCGCCTGGAGTTCTTCGTR: GTGTTGTCCTTTTCAGGCATTTCShc1F: GCCGACTGCAAACAGATCATTR: CCACCGGACGCGAAAGSnai2F:CACACAGTTATTATTTCCCCATATCTCTR:CGAGGTGAGGATCTCTGGTTTTSoat1F: AGAATATCCCACGAGTACTAAATGCAR: GGTACTGGTTGACTGTGGGTAGTGStat1F: TGAGCCCGACCCTATTACAAAAR: CCACGAAGGAGCTCTGAATGAZhx2F: AACTCACAGCAGACACTGATAGGAAR:TCAGAAGGATTTCAGCACAGACA

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