[发明专利]一种包含NT-proBNP抗原结合结构域的结合蛋白

权利要求书

1.一种包含NT-proBNP抗原结合结构域的分离的结合蛋白，其特征在于，所述抗原结合结构域包括互补决定区CDR-VH1、互补决定区CDR-VH2、互补决定区CDR-VH3、互补决定区CDR-VL1、互补决定区CDR-VL2和互补决定区CDR-VL3；

互补决定区CDR-VH1为G-Y-X1-F-T-X2-Y-Y-I-X3，其中，X2是D；

互补决定区CDR-VH2为W-I-X1-P-E-X2-F-N-X3-E-Y-N-E-X4-F-K-G，其中，X4是K；

互补决定区CDR-VH3为A-X1-D-X2-D-W-X3-G-D，其中，X1是R；

互补决定区CDR-VL1为R-X1-S-Q-S-X2-X3-H-X4-N-G-N-T-Y-L-H，其中，X1是S；

互补决定区CDR-VL2为K-X1-S-X2-R-F-S，其中，X2是D；

互补决定区CDR-VL3为X1-Q-X2-T-H-X3-P-P-T，其中，X1是S；

各互补决定区的突变位点选自下述突变组合中的任一种：

位点	CDR-VH1 X1/X3	CDR-VH2 X1/X2/X3	CDR-VH3 X2/X3	CDR-VL1 X2/X3/X4	CDR-VL2 X1	CDR-VL3 X2/X3
突变 1	F/Q	Q/D/V	A/E	I/L/R	V	T/V
突变 1-1	F/H	Q/E/V	P/E	I/L/K	V	T/I
突变 1-2	F/N	Q/E/L	A/D	I/V/R	L	S/L
突变 1-3	V/Q	Q/D/L	A/E	I/V/K	V	T/L
突变 1-4	V/H	Q/D/I	G/D	I/I/R	L	S/V
突变 1-5	V/N	Q/D/V	G/E	I/I/K	I	T/V
突变 1-6	T/Q	H/E/I	G/D	L/L/R	L	S/I
突变 1-7	T/H	H/E/V	G/E	L/L/K	I	T/I
突变 1-8	T/N	H/E/L	A/D	L/V/R	V	S/L
突变 1-9	V/Q	H/D/L	A/E	L/V/K	I	T/L
突变 1-10	V/H	H/D/I	P/D	L/I/R	V	S/V
突变 1-11	V/N	H/D/V	P/E	L/I/K	L	T/V
突变 1-12	T/Q	N/E/I	P/D	I/L/R	V	S/I
突变 1-13	T/H	N/E/V	P/E	I/L/K	L	T/I
突变 1-14	T/N	N/E/L	G/D	I/V/R	I	S/L
突变 1-15	F/Q	N/D/L	G/E	I/V/K	L	T/L
突变 1-16	F/H	N/D/I	A/D	I/I/R	I	S/V
突变 1-17	F/N	N/D/V	A/E	I/I/K	V	T/V
突变 1-18	T/Q	Q/E/I	A/D	L/L/R	I	S/I
突变 1-19	T/H	Q/E/V	A/E	L/L/K	V	T/I
突变 1-20	T/N	Q/E/L	G/D	L/V/R	L	S/L
突变 1-21	F/Q	Q/D/L	G/E	L/V/K	V	T/L
突变 1-22	F/H	Q/D/I	P/D	L/I/R	L	S/V
突变 1-23	F/N	Q/D/V	P/E	L/I/K	I	T/V
突变 1-24	V/Q	H/E/I	P/D	I/L/R	L	S/I
突变 1-25	V/H	H/E/V	P/E	I/L/K	I	T/I
突变 1-26	V/N	H/E/L	A/D	I/V/R	V	S/L
突变 1-27	F/Q	H/D/L	A/E	I/V/K	I	T/L
突变 1-28	F/H	H/D/I	G/D	I/I/R	V	S/V
突变 1-29	F/N	H/D/V	G/E	I/I/K	L	T/V
突变 1-30	V/Q	N/E/I	A/D	L/L/R	V	S/I
突变 1-31	V/H	N/E/V	A/E	L/L/K	L	T/I
突变 1-32	V/N	N/E/L	G/D	L/V/R	I	S/L
突变 1-33	T/Q	N/D/L	G/E	L/V/K	L	T/L
突变 1-34	T/H	N/D/I	P/D	L/I/R	I	S/V
突变 1-35	T/N	N/D/V	P/E	L/I/K	V	T/V
突变 1-36	F/Q	Q/E/I	A/D	I/L/R	I	S/I
突变 1-37	F/H	Q/E/V	A/E	I/L/K	V	T/I
突变 1-38	F/N	Q/E/L	G/D	I/V/R	L	S/L
突变 1-39	V/Q	Q/D/L	G/E	I/V/K	V	T/L
突变 1-40	V/H	Q/D/I	P/D	I/I/R	L	S/V
突变 1-41	V/N	Q/D/V	P/E	I/I/K	I	T/V
突变 1-42	T/Q	H/E/I	P/D	L/L/R	L	S/I
突变 1-43	T/H	H/E/V	P/E	L/L/K	I	T/I
突变 1-44	T/N	H/E/L	G/D	L/V/R	V	S/L
突变 1-45	F/Q	H/D/L	G/E	L/V/K	I	T/L
突变 1-46	F/H	H/D/I	A/D	L/I/R	V	S/V
突变 1-47	F/N	H/D/V	A/E	L/I/K	L	T/V
突变 1-48	V/Q	N/E/I	P/D	I/L/R	V	S/I
突变 1-49	V/H	N/E/V	P/E	I/L/K	L	T/I
突变 1-50	V/N	N/E/L	G/D	I/V/R	I	S/L
突变 1-51	T/Q	N/D/L	G/E	I/V/K	L	T/L
突变 1-52	T/H	N/D/I	A/D	I/I/R	I	S/V
突变 1-53	T/N	N/D/V	A/E	I/I/K	V	T/V
突变 1-54	F/Q	Q/E/I	G/D	L/L/R	I	S/I
突变 1-55	F/H	Q/E/V	G/E	L/L/K	V	T/I
突变 1-56	F/N	Q/E/L	A/D	L/V/R	L	S/L
突变 1-57	V/Q	Q/D/L	A/E	L/V/K	V	T/L
突变 1-58	V/H	Q/D/I	P/D	L/I/R	L	S/V
突变 1-59	V/N	Q/D/V	P/E	L/I/K	I	T/V
突变 1-60	T/Q	H/E/I	G/D	I/L/R	L	S/I
突变 1-61	T/H	H/E/V	G/E	I/L/K	I	T/I
突变 1-62	T/N	H/E/L	A/D	I/V/R	V	S/L
突变 1-63	F/Q	H/D/L	A/E	I/V/K	L	T/L
突变 1-64	F/H	H/D/I	P/D	I/I/R	V	S/V
突变 1-65	F/N	H/D/V	P/E	I/I/K	L	T/V
突变 1-66	V/Q	N/E/I	G/D	L/L/R	V	S/I
突变 1-67	V/H	N/E/V	G/E	L/L/K	L	T/I
突变 1-68	V/N	N/E/L	A/D	L/V/R	I	S/L
突变 1-69	T/Q	N/D/L	A/E	L/V/K	L	T/L

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