[发明专利]一种红花檵木查尔酮异构酶LcCHI基因及其应用

权利要求书

1.一种来源于红花檵木的查尔酮异构酶基因，其特征在于，所述查尔酮异构酶基因的核苷酸序列如下所示。

2.ATGGCTAAAG TACTGCACGT CACCGGAGTT CAGGTCGAGA ACGTTACATT CCCACCGACG 60

GTGAAGCCTC CTGGCTCGAC CAACACCTTG TTCCTCGGTG GCGCAGGGGA GAGGGGTTTG 120

GAAATTCAAG GGAAGTTCGT GAAGTTCACA GCCATAGGGG TTTACTTAGA AGATAATGCC 180

GTTCCGTCCC TCGCCGTTAA GTGGAAGGGG AAGACTGCGG AGGAGTTGAC GGAATCCGTC 240

GAGTTCTTCA GGGATATCGT TACAGGTCCC TTTGAGAAAT TCACACGGGT GACTATGATC 300

TTGCCATTAA CTGGGCAGCA ATATTCAGAG AAGGTGGCAG AAAATTGCGT CGCCATTTGG 360

AAAGCTCTGG GAATTTACAC TGATGCAGAG GCCAAAGCCA TTGAGAAGTT CACTGAGGTC 420

TTCAAGGATG AAACCTTTCC ACCTGGTGGC TCTATTCTTT TCACACAGTC ACCCCTCGGA 480

TCATTAACGA TTGGCTTCTC CAAAGATGGG TGTGTACCAG AAGTTGGGAA GGCAGTAATA 540

GAGAATAAAC TACTTTCAGA GGCAGTACTG GAGTCAATCA TAGGCAAGCA TGGTGTTTCT 600

CCTGTAGCCA AGCAGTCTTT GGCTACTAGA TTATCTGAAT TATTGAAGGA GGCCTAG 657。

3.如1所示的红花檵木的查尔酮异构酶基因，其氨基酸序列如下所示：

Met Ala Lys Val Leu His Val Thr Gly Val Gln Val Glu Asn Val Thr Phe ProPro Thr Val Lys Pro Pro Gly Ser Thr Asn Thr Leu Phe Leu Gly Gly Ala Gly GluArg Gly Leu Glu Ile Gln Gly Lys Phe Val Lys Phe Thr Ala Ile Gly Val Tyr LeuGlu Asp Asn Ala Val Pro Ser Leu Ala Val Lys Trp Lys Gly Lys Thr Ala Glu GluLeu Thr Glu Ser Val Glu Phe Phe Arg Asp Ile Val Thr Gly Pro Phe Glu Lys PheThr Arg Val Thr Met Ile Leu Pro Leu Thr Gly Gln Gln Tyr Ser Glu Lys Val AlaGlu Asn Cys Val Ala Ile Trp Lys Ala Leu Gly Ile Tyr Thr Asp Ala Glu Ala LysAla Ile Glu Lys Phe Thr Glu Val Phe Lys Asp Glu Thr Phe Pro Pro Gly Gly SerIle Leu Phe Thr Gln Ser Pro Leu Gly Ser Leu Thr Ile Gly Phe Ser Lys Asp GlyCys Val Pro Glu Val Gly Lys Ala Val Ile Glu Asn Lys Leu Leu Ser Glu Ala ValLeu Glu Ser Ile Ile Gly Lys His Gly Val Ser Pro Val Ala Lys Gln Ser Leu AlaThr Arg Leu Ser Glu Leu Leu Lys Glu Ala。

4.含要求1所述的查尔酮异构酶基因的重组质粒。