[发明专利]用于细菌asd基因平衡致死系统的表达质粒及其应用

权利要求书

1.一种用于细菌asd基因平衡致死系统的表达质粒，其特征在于，所述用于细菌asd基因平衡致死系统的表达质粒为pCW1910质粒，包含Trc启动子，多克隆位点，rrnB T1终止子，rrnB T2终止子，bom区域，ori复制起始位点，β内酰胺酶和asd基因；pCW1910质粒的核苷酸序列为

AATGAGCTGTTGACAATTAATCATCCGGCTCGTATAATGTGTGGGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGACCATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAATTCGCAATTCCCGGGGATCCGTCGACCTGCAGCCAAGCTCCCAAGCTTTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTCACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGACTAGTTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGACTAGTCTACGCCAACTGGCGCAGCATTCGACGCAGCGGCTCGGCGGCGCCCCATAACAACTGGTCGCCTACGGTAAACGCCGACAAGAACTCTGGCCCCATGTTCAGCTTACGCAGACGACCAACCGGCGTAGTCAACGTGCCGGTCACCGCCGCCGGGGTTAATTCGCGCATAGTGATATCACGATCGTTCGGCACCACTTTCGCCCACGGATTATGTGCCGCCAGCAGTTCTTCCACCGTCGGAATGGATACCTCTTTTTTCAGCTTGATGGTGAACGCCTGGCTGTGACAGCGCAGCGCGCCGACGCGCACGCACAAACCATCAACCGGAATCACAGAGGCAGTATTGAGAATCTTGTTGGTTTCCGCCTGGCCTTTCCACTCTTCGCGGCTCTGGCCGTTATCGAGCTGTTTGTCGATCCAGGGGATCAGGCTTCCCGCCAGCGGTACGCCAAAGTTATCAACCGGCAGCTCGCCGCTGCGGGTCAATGCCGTAACTTTGCGTTCAATATCAAGAATTGCGGAAGACGGCGTCGCCAGTTCATCGGCGACATGGCCATACAACTGACCCATCTGGGTTAACAGCTCGCGCATATGGCGCGCGCCGCCGCCGGAGGCGGCCTGATAGGTCGCGACGGATACCCAGTCAACGAGATTATGGGCAAAGAGACCGCCCAGCGACATCAACATCAGGCTAACGGTACAGTTACCGCCCACAAAGGTCTTCACGCCATTGTTCAGGCCGTCGGTAATCACGTCCTGGTTAACCGGGTCGAGAATAATAATGGCATCATCTTTCATGCGCAGCGTAGAAGCCGCGTCAATCCAGTAACCCTGCCATCCGCTTTCGCGCAGCTTTGGATAAATTTCGTTGGTATAATCGCCGCCCTGGCAGGTCACGATGATATCGAGCGCTTTTAGCGCATCCAGATCAAAAGCGTCCTGTAGCGTGCCGGTGGAGGTGTCGCCGAAGGTGGGCGCCGCCTGTCCAAACTGGGAGGTAGAAAAGAAAACAGGGCGAATAGCGTCGAAATCGCGCTCCTCTACCATGCGTTGCATGAGAACAGAGCCGACCATTCCGCGCCAGCCGATAAAACCAACATTTTTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG。