[发明专利]一种检测耳聋基因21位点的试剂盒有效
| 申请号： | 202110767870.1 | 申请日： | 2021-07-07 |
| 公开（公告）号： | CN113481291B | 公开（公告）日： | 2023-03-24 |
| 发明（设计）人： | 余艳;陈鹏;谭兵健;代冰;唐春燕;葛虎 | 申请（专利权）人： | 长沙金域医学检验实验室有限公司 |
| 主分类号： | C12Q1/6883 | 分类号： | C12Q1/6883;C12Q1/6858 |
| 代理公司： | 长沙优企知识产权代理事务所(普通合伙) 43243 | 代理人： | 周栋 |
| 地址： | 410000 湖南省长沙市高新开发区麓天路*** | 国省代码： | 湖南;43 |
| 权利要求书： | 查看更多 | 说明书： | 查看更多 |
| 摘要： | |||
| 搜索关键词： | 一种 检测 耳聋 基因 21 试剂盒 | ||
1.一种检测耳聋基因21个位点的试剂盒，其特征在于，所述试剂盒能同时检测耳聋基因的如下位点：检测GJB2基因rs80338939，rs104894404，rs80338943，rs111033204，rs80338942，rs773528125；GJB3基因rs74315319，rs74315318；MITO-12SrDNA基因rs267606617，rs267606619；SLC26A4基因外显子9 的rs201562855，rs111033305，rs111033220；SLC26A4基因外显子2的rs1057516953；SLC26A4基因外显子14的rs192366176；SLC26A4基因外显子16的rs200455203，rs111033318；SLC26A4基因外显子18的rs121908363，rs121908362；SLC26A4基因外显子4的rs111033380；SLC26A4基因外显子6/7 的rs111033313共21个位点；所述试剂盒中包括如下全部扩增引物：
DFN.26A4.Ex2.Fwd ：TCAAGGAATGGCTGCTTAGTGA；
DFN.26A4.Ex2.Rev ：GACCTATGGTAGCTGGGGAG；
DFN.GJB3.Fwd ：GCCTGGTGCAGTGTGCCAA；
DFN.GJB3.Rev ：TCGAGGCTTGTCCTTGTGC；
DFN.12SrRNA.Fwd ：GGCTACAAAGTAAGCGCAAGTAC；
DFN.12SrRNA.Rev ：GCCAGGTTTCAATTTCTATCG；
DFN.26A4.Ex9.Fwd ：CGTTGTCATCCAGTCTCTTCCTTAG；
DFN.26A4.Ex9.Rev ：CTCGACTTGTTCTCTGAGATGG；
DFN.GJB2.F1：TGGGGAAGTAGTGATCGTAGC；
DFN.GJB2.R1：GTAAGAGTTGGTGTTTGCTCA；
DFN.26A4.Ex14.Fwd ：CCTCAAGGAGTGAAGATTCTTAG；
DFN.26A4.Ex14.Rev ：GACTGTAAATTATTTGAAGGCAAGG；
DFN.26A4.Ex4.Fwd ：CGACGAACACTTTCTCGTATCC；
DFN.26A4.Ex4.Rev ：TTACTATTTTAGCACCTGACCT；
DFN.26A4.Ex16.Fwd ：GAGATTCAAGTGGATTGGAACTCTG；
DFN.26A4.Ex16.Rev：ATTACAAAGCCCATGTATTTGCC；
DFN.26A4.Ex18.Fwd ：GAAGATTATGTGATAGAAAAGCTGG；
DFN.26A4.Ex18.Rev ：GTTTCTAAAATGGAACCTTGACC；
DFN.GJB2.F2：ACAAAGCAGTCCACAGTGTTG；
DFN.GJB2.F2：GGCTGCAAGAACGTGTGCTAC；
DFN.26A4.Ex67.F1：CACAAAATCCCAGTCCCTATTCC；
DFN.26A4.Ex67.R1：AGTACTAAGAGGAACACCACACTC；
所述试剂盒中还包括如下全部延伸引物：
S.DFN-35delG ：GGAGTGTTTGTTCACACCCCC；
S.DFN-1229：TTTTTCCACCACTGCTCTTTCCCGCA；
S.DFN-1494：TTTTTTTTTTTTTTGCGTACACACCGCCCGTCAC；
S.DFN-del16：TTTTTTTTTTTTTTTTTTTTCTGCAACACCCTGCAGCCAG；
S.DFN-547：TTTTTTTTTTTTTTTTTTGTAGGTGAAGATTTTCTTCT；
S.DFN-281：TTTTTTTTTTTTTTTTTTTTACCTTGCAGCGTGGCCACTAGCCCA；
S.DFN-1226：TTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGGCCACCACTGCTCTTTCCC；
S.DFN-1174：TTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGAGAAGAATCCTGAGAAGATGT；
S.DFN-538：TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATCGTGGACTGCTACATTGCC；
S.DFN-1555：TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCTACGCATTTATATAGAGGAG；
S.DFN-167delT ：TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCGACTTTGTCTGCAACACCC；
S.DFN-1707：TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATGTATCAAGTCCACAGTAA；
S.DFN-589：TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAGTGCCCTGACTCTGCTGGTT；
S.DFN-919：TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAATGGCAGTAGCAATTATCGTC；
S.DFN-2027：TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCAGAACCTTACCACCCGC；
S.DFN-2162：TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACATTAGAAAGGACACATTCTTTTTGA；
S.DFN-dupAACG ：TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCATGCAGCGGCTGGTGAAGTGCAACG；
S.DFN-1975：TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATATAGCTCCACAGTCAAGCA；
S.DFN-235delC ：TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGACACGAAGATCAGCTGCAGG；
S.DFN-B.2168：TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTGGTTCTGTAGATAGAGTATAGCATCA；
S.DFN-B.delAT ：TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCATGCACGTGGCCTACCGGAGAC。
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