[发明专利]盖塔病毒全基因组序列、扩增方法及引物

摘要

盖塔病毒全基因组序列、扩增方法及引物，该基因组全长包括11690个核苷酸，不包括5’端帽子结构、3’端的多聚核苷酸尾，整个基因组编码一个多聚蛋白，5’端2/3编码非结构蛋白，3’端1/3编码结构蛋白；扩增用引物覆盖整个病毒基因组，扩增片段长度为900-1000个碱基，相邻片段之间有70-120个碱基的重叠区，所有引物不包含简并碱基，100％与盖塔病毒序列相同，引物的3’端为C，G或T保证稳定地与cDNA序列结合，所有引物的退火温度，GC含量基本相同，从而保证所有引物能够在相同的PCR条件下进行扩增反应。本发明的盖塔病毒全基因组序列为制备盖塔病毒全基因组克隆，研究盖塔病毒结构与功能的关系、盖塔病毒与宿主相互关系及对盖塔病毒的预防与控制打下了基础。

主权项

盖塔病毒全基因组序列，其特征在于：基因组全长包括11690个核苷酸，不包括5’端帽子结构、3’端的多聚核苷酸尾，整个基因组编码一个多聚蛋白，5’端2/3编码非结构蛋白，3’端1/3编码结构蛋白，序列如下：ATGGCGGACGTGTGACATCACCGTTCGCTCTTTCTAGGATCCTTTGCTACTCCACATAGTGAGAGACAAACAACCCAAATGAAGGTAACCGTGGACGTTGAGGCTGATAGCCCATTCCTTAAGGCCCTTCAGAAGGCGTTTCCCGCCTTTGAGGTTGAATCACAGCAGGTCACACCGAATGACCATGCTAACGCTAGAGCATTTTCGCATCTGGCTACTAAACTGATTGAGCAAGAGGTTCCAACAGGCGTCACCATCCTGGACGTGGGTAGTGCACCCGCAAGGAGGTTGATGTCTGACCACACCTACCACTGCATCTGCCCCATGAAAAGTGCGGAAGACCCAGAGAGACTGGCGAATTACGCTCGAAAGCTGGCGAAAGCATCGGGGACTGTGCTAGACAAGAATGTGTCCGGAAAGATAACGGACCTACAAGACGTCATGGCCACTCCAGACTTGGAATCCCCGACTTTTTGCCTGCATACTGACGAGACGTGCCGCACTAGGGCTGAGGTCGCCGTGTACCAGGACGTATACGCTGTGCACGCACCGACGTCACTGTACCACCAGGCCATCAAAGGCGTCAGGACGGCGTATTGGATTGGATTCGACACCACTCCATTCATGTTCGAGGCACTAGCGGGCGCGTACCCTGCGTACTCGACCAACTGGGCAGATGAGCAAGTGCTGCAAGCTCGTAACATCGGCCTGTGCGCGACAGGCCTCTCCGAGGGGCGTCGCGGCAAACTCTCCATTATGAGAAAAAAGTGCTTGCGACCGAGCGACAGAGTAATGTTTTCGGTCGGGTCCACCTTGTACACCGAGAGCCGAAAGCTGCTGCGCAGCTGGCATTTACCTTTCGTGTTTCACCTGAAGGGTAAGAACAGTTTTACCTGCAGGTGCGACACGGTGGTGTCATGCGAAGGTTATGTGGTAAAGAAGATCACCATAAGCCCGGGCATATATGGAAAAACAGTCGATTACGCAGTTACCCATCACGCAGAGGGTTTCCTGATGTGTAAGATCACTGATACAGTCAGAGGAGAAAGAGTCTCTTTCCCGGTCTGTACCTATGTGCCTGCAACCATATGCGACCAGATGACGGGTATACTTGCCACTGATGTGACACCAGAGGATGCCCAGAAGCTCCTGGTTGGATTGAACCAACGCATAGTGGTGAACGGTAGGACGCAAAGAAACACAAACACAATGAAAAACTACCTACTGCCAGTGGTAGCGCAAGCATTCAGTAAATGGGCACGAGAGGCACGCGCAGACATGGAGGACGAAAAACCCCTAGGCACCAGAGAACGCACGTTGACGTGTTGTTGCCTGTGGGCGTTCAAAAGCCACAAAACCCACACCATGTATAAGCGGCCTGAAACACAAACTATCGTCAAAGTGCCTTCCACTTTTGACTCCTTTGTGATACCGAGCCTGTGGTCATTCAGTCTTTCCATGGGTATCAGACAGAGGATCAAATTGCTACTCAGCGCAAGAATGGCCCAAGGCCTACCATACTCAGGAGATCGCACTGAAGCTCGCGCGGCAGAAGAAGAAGAGAAGGAGGTGCAGGAGGCTGAACTTACGAGGGCAGCGCTGCCACCGCTAGTGAGCGGCTCTTGTGCTGACGATATCGCCCAGGTGGATGTAGAGGAACTAACCTTCAGAGCCGGAGCCGGGGTTGTGGAAACACCCAGGAATGCCCTGAAGGTTACACCGCAAGCACACGACCATCTCATAGGCTCCTACTTGATCCTTTCCCCCCAAACGGTGTTGAAAAGCGAGAAGCTGGCACCCATCCACCCTCTTGCTGAGCAAGTCACGGTCATGACCCACTCTGGAAGATCCGGCAGATACCCAGTCGACAAGTACGACGGACGGGTATTGATCCCAACAGGAGCAGCCATCCCAGTGAGTGAGTTCCAGGCACTCAGCGAGAGCGCAACCATGGTGTACAATGAGAGGGAATTTATAAATCGCAAGCTACACCACATAGCGCTATACGGGCCAGCCTTGAATACCGACGAGGAAAGCTACGAAAAAGTGAGAGCCGAAAGGGCAGAGACAGAGTATGTGTTCGACGTGGACAAGAAGGCATGTATCAAGAAGGAGGAGGCATCAGGCCTTGTGTTAACAGGGGACCTAATCAATCCACCTTTCCACGAATTCGCATACGAAGGACTCAAGATCCGCCCAGCAGCCCCGTACCACACGACGATCATTGGTGTGTTTGGCGTTCCGGGTTCGGGCAAGTCGGCTATCATTAAGAATATGGTGACGACTCGCGATCTGGTGGCCAGTGGAAAGAAGGAGAACTGCCAAGAGATCATGAATGATGTAAAGAGGCAA CGCGGGTTGGACGTAACCGCTAGGACCGTCGACTCAATCTTATTGAATGGGTGCAAGAAAGGCGTAGAAAACCTTTACGTCGATGAGGCGTTCGCGTGTCACTCGGGTACTTTGCTAGCGCTCATCGCGCTGGTGAGACCGTCAGGTAAGGTAGTACTGTGCGGCGACCCTAAGCAGTGTGGTTTCTTCAATTTGATGCAACTGAAGGTGCACTATAACCACAACATATGTACAAGGGTGCTCCATAAGAGCATCTCCAGAAGATGCACTCTACCTGTTACGGCGATCGTGTCCACCCTGCACTACCAAGGGAAGATGAGAACGACGAACCGATGCAACACCCCCATTCAGATTGACACCACCGGTTCCTCCAAACCAGCCTCAGGAGATATCGTGTTAACGTGCTTCCGCGGCTGGGTGAAGCAACTGCAAATCGACTATCGTGGACACGAGGTGATGACCGCAGCCGCTTCCCAGGGTCTGACAAGGAAAGGCGTGTACGCCGTGAGACAGAAAGTGAACGAAAACCCACTGTACTCACCTCTGTCGGAGCACGTCAACGTGCTGTTGACCCGAACTGAAAACCGACTGGTGTGGAAGACACTGTCGGGTGACCCGTGGATAAAGGTGTTAACCAATGTTCCACGTGGGGATTTCAGTGCAACTCTGGAGGAATGGCAAGAAGAACATGACGGTATCATGAGAGTGTTGAACGAGCGACCGGCGGAGGTCGATCCATTCCAAAACAAGGCTAAGGTGTGCTGGGCAAAATGTCTGGTGCAAGTTCTTGAGACGGCCGGAATACGTATGACGGCAGATGAATGGAACACCATCTTGGCTTTCAGAGAGGACAGAGCGTACTCACCAGAAGTCGCTCTCAACGAGATTTGCACTCGTTACTACGGCGTTGACCTAGACAGCGGCCTATTCTCAGCGCAGTCGGTTTCCCTCTTTTATGAGAACAACCATTGGGACAACAGGCCTGGAGGACGCATGTACGGGTTCAACCATGAAGTAGCCAGGAAATACGCAGCCAGGTTTCCATTTCTACGTGGCAACATGAACTCGGGGCTACAACTAAATGTCCCTGAGAGGAAGCTCCAGCCCTTTAGCGCTGAATGCAACATAGTCCCATCCAATCGTCGGTTACCGCATGCTCTGGTCACAAGTTATCAGCAGTGCCGTGGGGAGAGGGTAGAGTGGTTGCTGAAAAAGATTCCAGGTCACCAAATGTTACTCGTAAGTGAGTACAACCTGGTGATACCTCACAAAAGAGTCTTCTGGATTGCACCTCCGCGGGTGTCAGGCGCGGACCGCACGTACGACTTGGACCTAGGGTTACCTATGGATGCAGGCCGTTACGACCTGGTATTCGTCAACATCCATACTGAGTACCGGCAACACCACTACCAACAATGCGTCGACCATTCAATGCGCCTGCAGATGCTGGGAGGGGATTCACTACACCTGCTCAGACCAGGAGGCTCGCTGCTGATGAGAGCATATGGTTACGCAGACAGAGTCAGCGAGATGGTGGTGACAGCCCTGGCTAGGAAATTCTCGGCGTTCCGTGTCCTGAGACCGGCGTGTGTGACGAGCAACACAGAAGTGTTCCTGCTGTTTTCTAACTTTGATAACGGCAGAAGAGCGGTAACCTTGCACCAAGCTAACCAAAAACTTAGCTCAATGTATGCCTGCAACGGATTGCACACTGCTGGTTGTGCACCGTCATACAGGGTCCGCCGCGCAGATAATTCAGGACACAGTGAGGAAGCGGTCGTAAATGCTGCCAATGCCAAAGGTACCGTGAGCGACGGAGTGTGCAGGGCGGTTGCTAAGAAGTGGCCATCATCTTTCAAAGGGGCTGCAACTCCAGTCGGCACAGCCAAAATGATCCGCGCAGATGGCATGACCGTAATCCACGCAGTGGGACCAAACTTCTCCACCGTAACAGAAGCCGAAGGGGACAGAGAGCTAGCGGCCGCGTATCGAGCTGTGGCTAGCATAATTAGTACCAATAACATAAAGAGCGTCGCTGTACCGCTGCTGTCCACAGGCACCTTCTCCGGCGGTAAGGACAGAGTGATGCAGTCCTTGAACCACTTATTCACGGCACTGGACGCAACCGACGCAGACGTGGTTATCTACTGCAGAGATAAAAACTGGGAAAAGAAGATTCAGGAAGCCATCGACAGGCGGACGGCAATCGAGCTCGTATCTGAAGACGTGACCTTGGAAACCGATCTGGTTAGGGTACACCCGGACAGTTGCTTAGTCGGCAGAAATGGTTACAGTGCAACTGACGGTAAACTGTACTCTTACCTTGAGGGCACGAGGTTCCACCAGACGGCGGTCGACATGGCTGAAATATCAACTTTATGGCCAAGACTCCAAGATGCTAACGAGCAGATCTGCCTGTACGCCCTAGGGGAGACGATGGACAGCATACGCACTAAATGCCCAGTAGAGGACGCCGATTCGTCTACGCCGCCGAAAACGGTACCGTGTCTATGTCGGTATGCGATGACCGCGGAGCGGGTTGCCAGACTTAGGATGAATAACACCAAAAACATCATCGTGTGCTCCTCCTTTCCATTACCGAAGTACAGGATAGAAGGCGTGCAGAAGGTGAAGTGTGACCGAGTGCTAATTTTTGACCAGACCGTCCCGTCACTAGTAAGTCCCAGAAAGTACATACAGCAGCCGCCGGAACAGCTGGATAATGTGAGCCTGACTTCTACGACGTCGACGGGATCCGCATGGTCAT TTCCATCGGAAACGACCTACGAAACCATGGAAGTCGTAGCCGAGGTACACACCGAACCTCCAATCCCTCCGCCTCGCCGACGTAGAGCAGCCGTCGCCCAACTTAGACAGGATCTGGAAGTCACCGAGGAGATCGAGCCGTACGTGACACAGCAAGCAGAGGTCGTGGTCATGGAGAGGGTCGCGACGACAGACATACGCGCTATCCCAGTCCCGGCACGGCGGGCCATTACAATGCCAGTCCCAGCCCCCAGGGTTCGTAAGGTCGCTACTGAACCTCCATTAGAACCGGAAGCTCCTATCCCGGCACCAAGAAAGAGAAGAACCACTAGCACCGCACCTCCGCATAACCCCGAGGATTTCGTTCCCAGGGTACCTGTTGAGTTACCGTGGGAGCCGGAAGACCTAGACATCCAATTCGGTGACTTGGAGCCACGCCGCCGGAACACCAGGGACCGAGATGTCAGCACAGGAATACAGTTCGGTGACATCGACTTTAACCAGTCCTGACTAGGCAGGGCTGGCGCGTATATCTTTTCGTCTGACACTGGCCCGGGTCACCTACAACAGAAGTCCGTAAGGCAACATGAATTGCCATGCGAGACTCTGTACGCCCATGAAGACGAACGCATATACCCGCCGGCATTTGACGGAGAGAAAGAAAAAGTACTCCAGGCAAAGATGCAGATGGCCCCGACAGAAGCGAATAAGAGCAGGTACCAGTCGAGGAAAGTAGAGAACATGAAGGCATTAATTGTAGAAAGATTACGCGAAGGAGCAAAGTTGTACCTCCATGAGCAAACCGACAAAGTACCCACGTACACCAGCAAGTACCCTAGACCTGTGTACTCACCATCGGTGGATGACAGCCTGAGCGATCCGGAAGTGGCTGTGGCCGCCTGTAACTCTTTCTTAGAGGAGAATTATCCAACCGTGGCGAACTACCAGATAACCGATGAGTATGACGCTTATCTGGACTTGGTCGACGGCTCTGAAAGCTGCCTCGACAGAGCTACGTTCTGCCCGGCCAAACTAAGATGTTACCCTAAGCACCACGCATACCACCAACCACAAATCAGGAGCGCAGTACCTTCCCCTTTTCAAAACACGCTACAAAACGTGCTAGCCGCGGCCACTAAAAGAAATTGCAACGTCACCCAAATGAGAGAATTACCAACCATGGACTCTGCGGTGTTCAACGTAGAAAGCTTCAAAAAATACGCATGTACCGGCGAATATTGGCAAGAATTTAAAGACAATCCTATACGGATCACCACCGAAAACATAACGACGTACGTGGCTAAACTCAAGGGTCCAAAGGCTGCTGCCCTTTTTGCCAAGACGCATAACCTGGTGCCGCTTCAGGAGGTGCCAATGGACCGCTTCGTGATGGATATGAAGAGAGATGTGAAAGTTACACCAGGCACCAAGCATACCGAAGAAAGGCCAAAAGTGCAAGTTATTCAAGCGGCGGAACCATTGGCCACGGCATATTTATGCGGAATCCACAGAGAGTTAGTCAGGCGGCTAAAAGCCGTTCTGACCCCGAACATCCATACTCTGTTTGACATGTCGGCGGAGGACTTTGATGCCATCATAGCGGCACATTTCCAACCGGGAGATGCTGTACTGGAGACAGATATCGCATCCTTCGACAAGAGCCAAGACGACTCCTTAGCGCTAACAGCGCTGATGCTTCTGGAAGACCTCGGGGTCGACCAAGAACTGCTGGACCTTATCGAAGCTGCGTTTGGTGAGATCACGAGTGTGCATCTACCTACCGGTACAAGATTTAAATTCGGTGCTATGATGAAGTCAGGAATGTTTCTTACACTCTTCATCAACACGCTGCTGAACATTGTCATAGCGTGCCGCGTCTTACGCGACAAATTATCGTCCTCGGCGTGCGCCGCCTTCATAGGTGATGACAACATAGTGCACGGCGTGAGGTCAGACCCGCTAATGGCAGAAAGGTGTGCGAGTTGGGTCAACATGGAAGTAAAGATCATCGATGCCACAATGTGTGAGAAACCACCGTACTTTTGTGGAGGATTCATCCTGTACGACAGTGTCGCCGGTACAGCGTGTAGGGTTGCAGACCCGTTAAAGAGGCTGTTCAAACTCGGGAAACCGCTCCCGGCGGACGACAACCAGGATGAAGACAGAAGAAGGGCACTAAAAGATGAAACAGTTAAGTGGTCCCGCATAGGATTGAGAGAAGAATTAGACGTGGCATTGAGCTCAAGATACCAAGTCAGTGGCGTCGGAAACATCACTAGAGCGATGTCCACGCTGTCTAAGAGTTTGAAGTCTTTTAGGAAAATAAGAGGTCCCATCATACATCTGTACGGCGGTCCTAAATAGATGCAGGATTACACTACATCTAAAGACCACGTATTACAGACACCATGAATTACATTCCAACTCAAACCTTTTACGGACGCCGTTGGCGACCACGCCCGGCGTACCGTCCATGGCGGGTGCCGATGCAGCCGGCCCCACCCATGGTGATTCCTGAGCTGCAAACTCCGATCGTCCAGGCCCAACAGATGCAGCAGCTAATCAGTGCAGTTTCTGCCCTGACGACCAAGCAAAATGGCAAAGCACCGAAGAAGCCGAAGAAAAAGCCGCAAAAAGCGAAGGCTAAGAAAAACGAACAGCAAAAGAAGAACGAGAACAAGAAACCACCACCTAAGCAGAAGAATCCGGCTAAGAAGAAGAAACCAGGAAAAAGGGAACGCATGTGCATGAAGATAGAGAATGATTGC ATCTTCGAGGTCAAGCTTGACGGTAAGGTCACGGGATACGCCTGCCTAGTCGGGGATAAAGTGATGAAGCCGGCACACGTCAAAGGTGTGATCGACAACCCCGATCTAGCGAAGCTTACCTACAAGAAATCGAGTAAGTATGACCTGGAGTGCGCCCAGATACCAGTGCACATGAAGTCAGATGCTTCAAAGTACACCCATGAAAAACCGGAAGGGCACTACAATTGGCATCACGGTGCAGTGCAGTACAGCGGTGGCAGGTTCACAATCCCGACAGGCGCAGGTAAACCAGGAGACAGCGGCCGGCCGATCTTCGACAACAAAGGACGCGTGGTGGCCATTGTCCTGGGAGGGGCCAACGAAGGAGCCAGGACTGCCCTATCCGTCGTGACCTGGACCAAAGACATGGTCACACGGTACACCCCAGAAGGAACAGAAGAATGGTCTGCCGCCTTGATGATGTGCGTCCTAGCCAACGTTACATTCCCATGCTCAGAGCCCGCGTGTGCACCCTGTTGCTATGAAAAACAACCAGAACAGACACTGAGGATGTTAGAGGACAACGTGGACCGCCCGGGCTACTACGACCTGCTCGAGGCCACGATGACGTGTAACAACAGTGCACGCCACCGTCGCAGTGTGACGGAACACTTCAATGTCTACAAGGCCACGAAACCGTATCTAGCGTATTGCGCGGACTGCGGAGACGGGCAGTTCTGTTACAGCCCGGTGGCTATAGAAAAAATTAGAGATGAGGCTTCCGATGGCATGATAAAAATCCAGGTCGCAGCGCAAATTGGCATCAACAAAGGAGGAACACACGAACACAACAAAATCAGGTACATCGCCGGGCATGACATGAAAGAGGCAAACCGGGATTCTTTACAAGTGCATACTTCCGGTGTGTGCGCTATTCGAGGCACGATGGGCCACTTCATCGTGGCCTACTGCCCTCCAGGGGACGAACTAAAGGTCCAATTCCAAGATGCAGAATCGCACACCCAGGCCTGCAAAGTGCAGTACAAACACGCACCGGCCCCAGTAGGCAGAGAAAAATTCACCGTCAGGCCCCACTTCGGTATCGAAGTGCCATGCACAACGTACCAGCTGACTACCGCACCGACGGAGGAAGAGATCGACATGCATACCCCGCCGGATATCCCAGACATAACGTTGCTGTCGCAGCAGTCAGGCAATGTAAAGATCACAGCAGGAGGAAAAACCATCAGATACAACTGCACGTGTGGTAGTGGCAACGTGGGCACCACCAGTAGCGACAAGACTATCAATTCGTGCAAAATAGCACAGTGCCACGCTGCGGTGACTAACCACGATAAGTGGCAGTACACCTCCTCGTTTGTCCCTAGAGCCGACCAGTTGTCTCGCAAAGGTAAAGTGCACGTACCTTTCCCTCTGACCAACTCCACTTGCAGGGTGCCTGTCGCACGTGCACCAGGTGTCACATACGGAAAGAGAGAACTGACAGTGAAACTGCACCCAGATCATCCCACGCTGTTGACGTACCGGAGTCTAGGAGCGGATCCGCGTCCGTATGAGGAGTGGATAGACCGATACGTCGAACGGACCATACCGGTGACCGAAGATGGGATCGAGTACAGATGGGGAAACAACCCACCCGTGCGCTTGTGGGCCCAGCTGACAACTGAAGGCAAACCCCATGGGTGGCCGCACGAGATCATACTCTATTACTATGGACTATACCCAGCAGCCACCATCGCCGCCGTCTCAGCCGCGGGTCTCGCAGTCGTACTATCGCTGCTGGCGTCATGTTACATGTTCGCCACTGCACGCCGCAAGTGCCTGACCCCATACGCCCTGACCCCCGGAGCTGTCGTCCCGGTAACACTAGGAGTACTATGCTGCGCACCACGAGCGCATGCCGCGTCATTTGCGGAATCTATGGCGTATCTATGGGATGAGAATCAAACCCTGTTTTGGCTGGAGCTTGCAACGCCGCTCGCTGCCATAATCATACTTGTATGTTGCCTGAAGAACCTGCTTTGCTGCTGCAAACCGCTTTCTTTTTTAGTGCTGGTGAGCCTGGGAACTCCCGTCGTAAAATCTTACGAACACACCGCAACGATCCCGAATGTGGTGGGATTCCCGTATAAGGCTCACATTGAGAGGAACGGCTTCTCCCCGATGACCCTACAGCTTGAAGTACTAGGAACCAGCTTGGAACCCACGCTAAACTTAGAGTACATAACCTGTGAATACAAGACAGTCGTGCCATCACCTTATATCAAGTGCTGCGGGACATCAGAATGCAGATCCATGGAGCGCCCCGACTATCAATGTCAGGTCTACACAGGAGTGTACCCATTTATGTGGGGCGGCGCATACTGCTTCTGCGACACTGAGAACACCCAGCTGAGTGAAGCATACGTTGATAGATCGGACGTATGCAAGCACGACCATGCCGCCGCCTACAAGGCGCACACTGCGGCAATGAAAGCCACCATCCGAATAAGCTACGGGAACCTCAATCAGACAACAACGGCGTTCGTCAACGGGGAGCACACAGTGACCGTCGGAGGCAGCAGGTTTACTTTTGGTCCAATCTCCACTGCCTGGACGCCTTTCGACAACAAGATCGTCGTCTACAAGAACGACGTCTACAACCAGGACTTCCCACCCTACGGGTCAGGACAACCAGGGAGGTTTGGAGACATCCAGAGCAGGACGGTAGAGAGCAAGGACCTGTATGCCAACACCGCCCTCAAGTTGTCAAGACCTTCGTCCGG TACTGTTCACGTGCCTTACACACAGACCCCTTCTGGCTTTAAGTACTGGATAAAAGAGAGAGGCACGTCGCTGAATGACAAGGCTCCCTTTGGATGCGTAATCAAGACCAACCCAGTCAGAGCGGAAAATTGCGCCGTTGGCAACATCCCAGTCTCCATGGACATCCCGGACACCGCGTTTACGCGCGTGATTGATGCACCTGCCGTCACAAACCTGGAGTGCCAAGTGGCGGTCTGCACGCACTCATCGGACTTCGGCGGGATCGCGACTCTGACATTCAAAACTGACAAACCCGGAAAATGTGCTGTCCATTCTCATTCGAATGTAGCCACCATACAGGAGGCAGCTGTGGACATCAAAACAGATGGCAAGATAACCCTGCATTTCTCTACAGCATCAGCATCCCCGGCATTCAAGGTATCTGTGTGCAGTGCCAAAACGACATGCATGGCAGCGTGTGAGCCGCCGAAGGATCACATCGTCCCTTATGGGGCGAGCCATAACAACCAAGTTTTTCCTGACATGTCTGGCACGGCAATGACATGGGTGCAGCGGGTAGCCGGTGGACTCGGCGGGCTAACACTCGCCGCAGTGGCAGTACTTATATTGGTGACGTGTGTGACCATGCGCCGCTAACCGGGAGGCTTGACATAATGTATATATATAAGCATCATATTTTTAATAAAGCATATAAATAATCAAGTAGATCAAAGGGCTACCTAACCCCTGAATAGTAACAAAACGCAAAAATACAAAAACATTAGTTCAAAGGGCCAGTAACCCCTGAATAGTAACAAAATATAAAAACCAAAAACAGTAGTTCAAAGGGCTATACAACCCCTGAATAGTAACAAAATGCAGAAAAACCATAAAAATTATAAAATTAACTAATCAGATCATCTAAATTTGACTAATTGGAAATAGCCGAACTCTACGGAGATGTAGGCGTCCGAACTCCACGGAGACGTAGGACAAAATTCTGCCGAACCCCAGACCATCGGGGACGTAGGCGTCTAATTTGTTTTTTTAATATTTTAC。